The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Low fidelity DNA synthesis by human DNA polymerase-η
Abstract
A superfamily of DNA polymerases that bypass lesions in DNA has been described. Some family members are described as error-prone because mutations that inactivate the polymerase reduce damage-induced mutagenesis. In contrast, mutations in the skin cancer susceptibility gene XPV, which encodes DNA polymerase (pol)-eta, lead to increased ultraviolet-induced mutagenesis. This, and the fact that pol-eta primarily inserts adenines during efficient bypass of thymine-thymine dimers in vitro, has led to the description of pol-eta as error-free. However, here we show that human pol-eta copies undamaged DNA with much lower fidelity than any other template-dependent DNA polymerase studied. Pol-eta lacks an intrinsic proofreading exonuclease activity and, depending on the mismatch, makes one base substitution error for every 18 to 380 nucleotides synthesized. This very low fidelity indicates a relaxed requirement for correct base pairing geometry and indicates that the function of pol-eta may be tightly controlled to prevent potentially mutagenic DNA synthesis.
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“DNA pol h copies undamaged DNA with a lower fidelity than most DNA-directed polymerases with an average base-substitution error rate of 3.5 £ 10 ¡2 [22][23][24] .”
From DNA polymerase η mutational signatures are found in a variety of different types of cancer (2018) · cites this paper as [22]
In the citing paper
“The mutable motif of pol h, the short motif WA/TW (W = A or T) was delineated in the context of somatic hypermutations and in vitro systems 22,23 .”
From DNA polymerase η mutational signatures are found in a variety of different types of cancer (2018) · cites this paper as [22]
Citations
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Metadata
- Authors
- Matsuda, T., Bebenek, K., Masutani, C., Hanaoka, F., & Kunkel, T. A.
- Affiliations
- National Institutes of Health (US)
- The University of Osaka (JP)
- RIKEN (JP)
- Corresponding author
- Kunkel, Thomas A.
- Venue
- Nature
- Published
- 2000-04
- Volume
- 404
- Issue
- 6781
- Pages
- 1011-1013
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1581466567
- Cited by
- 372
- References
- 27
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 5 · 2023: 6 · 2022: 3 · 2021: 4 · 2020: 6 · 2019: 6 · 2018: 8 · 2017: 12 · 2016: 15 · 2015: 16 · 2014: 10 · 2013: 16 · 2012: 9
- FWCI
- 15.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Health Sciences › Medicine › Pathology and Forensic Medicine › Genetic factors in colorectal cancer
- Keywords
- DNA polymerase eta, DNA synthesis fidelity, error-prone DNA replication, thymine-thymine dimers, DNA damage bypass, proofreading exonuclease, XPA, mutagenesis
- MeSH terms
- DNA Polymerase iota, Y-Family DNA Polymerases, DNA, DNA Damage, DNA-Directed DNA Polymerase, DNA Repair, Humans, Mutagenesis
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