The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A bacteriophage encodes its own CRISPR/Cas adaptive response to evade host innate immunity
Open accessAbstract
Bacteriophages (or phages) are the most abundant biological entities on earth, and are estimated to outnumber their bacterial prey by tenfold. The constant threat of phage predation has led to the evolution of a broad range of bacterial immunity mechanisms that in turn result in the evolution of diverse phage immune evasion strategies, leading to a dynamic co-evolutionary arms race. Although bacterial innate immune mechanisms against phage abound, the only documented bacterial adaptive immune system is the CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) system, which provides sequence-specific protection from invading nucleic acids, including phage. Here we show a remarkable turn of events, in which a phage-encoded CRISPR/Cas system is used to counteract a phage inhibitory chromosomal island of the bacterial host. A successful lytic infection by the phage is dependent on sequence identity between CRISPR spacers and the target chromosomal island. In the absence of such targeting, the phage-encoded CRISPR/Cas system can acquire new spacers to evolve rapidly and ensure effective targeting of the chromosomal island to restore phage replication.
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Citations
Cites
The human gut virome: inter-individual variation and dynamic response to diet
Minot, S., Sinha, R., Chen, J., Li, H., Keilbaugh, S. A., Wu, G. D., Lewis, J. D., & Bushman, F. D. (2011) · Genome Research · doi:10.1101/gr.122705.111 · Cited by 935
Cited by
Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials
Bikard, D., Euler, C. W., Jiang, W., Nussenzweig, P. M., Goldberg, G. W., Duportet, X., Fischetti, V. A., & Marraffini, L. A. (2014) · Nature Biotechnology · doi:10.1038/nbt.3043 · Cited by 986
Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases
Citorik, R. J., Mimee, M., & Lu, T. K. (2014) · Nature Biotechnology · doi:10.1038/nbt.3011 · Cited by 808
CRISPR-Cas Systems: Prokaryotes Upgrade to Adaptive Immunity
Barrangou, R., & Marraffini, L. A. (2014) · Molecular Cell · doi:10.1016/j.molcel.2014.03.011 · Cited by 832
The CRISPR-Cas immune system: Biology, mechanisms and applications
Rath, D., Amlinger, L., Rath, A., & Lundgren, M. (2015) · Biochimie · doi:10.1016/j.biochi.2015.03.025 · Cited by 471
CRISPR-Cas: biology, mechanisms and relevance
Hille, F., & Charpentier, E. (2016) · Philosophical Transactions of the Royal Society B: Biological Sciences · doi:10.1098/rstb.2015.0496 · Cited by 442
Phage-mediated horizontal gene transfer and its implications for the human gut microbiome
Borodovich, T., Shkoporov, A. N., Ross, R. P., & Hill, C. (2022) · Gastroenterology Report · doi:10.1093/gastro/goac012 · Cited by 200
Mobile Genetic Elements and Evolution of CRISPR-Cas Systems: All the Way There and Back
Koonin, E. V., & Makarova, K. S. (2017) · Genome Biology and Evolution · doi:10.1093/gbe/evx192 · Cited by 176
Viruses and mobile elements as drivers of evolutionary transitions
Koonin, E. V. (2016) · Philosophical Transactions of the Royal Society B: Biological Sciences · doi:10.1098/rstb.2015.0442 · Cited by 161
Intestinal phages interact with bacteria and are involved in human diseases
Shuwen, H., & Kefeng, D. (2022) · Gut Microbes · doi:10.1080/19490976.2022.2113717 · Cited by 124
Transposon-encoded nucleases use guide RNAs to promote their selfish spread
Meers, C., Le, H. C., Pesari, S. R., Hoffmann, F. T., Walker, M. W. G., Gezelle, J., Tang, S., & Sternberg, S. H. (2023) · Nature · doi:10.1038/s41586-023-06597-1 · Cited by 60
“In the more recent evolutionary past, mature CRISPR–Cas systems have been repurposed by diverse types of mobile genetic elements, including plasmids 18 , phages 19 and transposons 20–22 .”
BackgroundInteraction of phages, bacteria, and the human immune system: Evolutionary changes in phage therapy
Jariah, R. O. A., & Hakim, M. S. (2019) · Reviews in Medical Virology · doi:10.1002/rmv.2055 · Cited by 40
CRISPR/Cas9-Mediated Phage Resistance Is Not Impeded by the DNA Modifications of Phage T4
Yaung, S. J., Esvelt, K. M., & Church, G. M. (2014) · PLoS ONE · doi:10.1371/journal.pone.0098811 · Cited by 39
Cas1 mediates the interference stage in a phage-encoded CRISPR–Cas system
Zhang, L., Wang, H., Zeng, J., Cao, X., Gao, Z., Liu, Z., Li, F., Wang, J., Zhang, Y., Yang, M., et al. (2024) · Nature Chemical Biology · doi:10.1038/s41589-024-01659-5 · Cited by 12
Boyd, C. M., Subramanian, S., Dunham, D. T., Parent, K. N., & Seed, K. D. (2023) · doi:10.7554/elife.87611.2 · Cited by 1
Self-citation
Metadata
- Authors
- Seed, K. D., Lazinski, D. W., Calderwood, S. B., & Camilli, A.
- Venue
- Nature
- Published
- 2013-02
- Volume
- 494
- Issue
- 7438
- Pages
- 489-491
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1976907618
- Cited by
- 386
- References
- 1
- FWCI
- 26.19
- Type
- journal article
- Language
- en
- Open access
- green
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
