Cited by 2,880Extracellular vesicles in diseaseCancer Genomics and DiagnosticsPancreatic and Hepatic Oncology Research

The DOI matches its Crossref record; 63 of the 63 references it cites were found in a registry or an index.

Glypican-1 identifies cancer exosomes and detects early pancreatic cancer

Open access

Melo, Sonia A., Luecke, Linda B., Kahlert, Christoph, Fernandez, Agustin F., Gammon, Seth T., Kaye, Judith, LeBleu, Valerie S., Mittendorf, Elizabeth A., Weitz, Juergen, Rahbari, Nuh, Reissfelder, Christoph, Pilarsky, Christian, Fraga, Mario F., Piwnica-Worms, David, Kalluri, Raghu·2015·Nature

DOI 10.1038/nature14581PMC PMC4825698

Open this paper in CoChat — free account

Abstract

Exosomes are lipid-bilayer-enclosed extracellular vesicles that contain proteins and nucleic acids. They are secreted by all cells and circulate in the blood. Specific detection and isolation of cancer-cell-derived exosomes in the circulation is currently lacking. Using mass spectrometry analyses, we identify a cell surface proteoglycan, glypican-1 (GPC1), specifically enriched on cancer-cell-derived exosomes. GPC1(+) circulating exosomes (crExos) were monitored and isolated using flow cytometry from the serum of patients and mice with cancer. GPC1(+) crExos were detected in the serum of patients with pancreatic cancer with absolute specificity and sensitivity, distinguishing healthy subjects and patients with a benign pancreatic disease from patients with early- and late-stage pancreatic cancer. Levels of GPC1(+) crExos correlate with tumour burden and the survival of pre- and post-surgical patients. GPC1(+) crExos from patients and from mice with spontaneous pancreatic tumours carry specific KRAS mutations, and reliably detect pancreatic intraepithelial lesions in mice despite negative signals by magnetic resonance imaging. GPC1(+) crExos may serve as a potential non-invasive diagnostic and screening tool to detect early stages of pancreatic cancer to facilitate possible curative surgical therapy.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1038/nature14581

Cites

63 of 63 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

1 retracted

RETRACTED: MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer
Taylor, D. D., & Gerçel-Taylor, Ç. (2008) · Gynecologic Oncology · doi:10.1016/j.ygyno.2008.04.033 · Cited by 2,333
Retracted
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860
Comparing the Areas under Two or More Correlated Receiver Operating Characteristic Curves: A Nonparametric Approach
DeLong, E. R., DeLong, D. M., & Clarke-Pearson, D. L. (1988) · Biometrics · doi:10.2307/2531595 · Cited by 22,649
Pancreatic Cancer
Spencer, P. M., Cameron, J. L., & Lin, L. (2000) · Cited by 7,202
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Théry, C., Amigorena, S., Raposo, G., & Clayton, A. (2006) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb0322s30 · Cited by 5,766
Exosomes: composition, biogenesis and function
Théry, C., Zitvogel, L., & Amigorena, S. (2002) · Nature reviews. Immunology · doi:10.1038/nri855 · Cited by 5,355
Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers
Skog, J., Würdinger, T., van Rijn, S., Meijer, D. H., Gainche, L., Curry, W. T., Carter, B. S., Krichevsky, A. M., & Breakefield, X. O. (2008) · Nature Cell Biology · doi:10.1038/ncb1800 · Cited by 5,001
Membrane vesicles as conveyors of immune responses
Théry, C., Ostrowski, M., & Segura, É. (2009) · Nature reviews. Immunology · doi:10.1038/nri2567 · Cited by 3,996
Mutation of the mouse klotho gene leads to a syndrome resembling ageing
Kuro-o, M., Matsumura, Y., Aizawa, H., Kawaguchi, H., Suga, T., Utsugi, T., Ohyama, Y., Kurabayashi, M., Kaname, T., Kume, E., et al. (1997) · Nature · doi:10.1038/36285 · Cited by 3,837
Ceramide Triggers Budding of Exosome Vesicles into Multivesicular Endosomes
Trajković, K., Hsu, C., Chiantia, S., Rajendran, L., Wenzel, D., Wieland, F., Schwille, P., Brügger, B., & Simons, M. (2008) · Science · doi:10.1126/science.1153124 · Cited by 3,760

Flagged first, then by citations

Show all 63 references

Sorted by citations

Items 201–300 of 1,005

Molecular Recognition-Based DNA Nanoassemblies on the Surfaces of Nanosized Exosomes
Wan, S., Zhang, L., Wang, S., Liu, Y., Wu, C. S., Cui, C., Sun, H. R., Shi, M., Jiang, Y. Y., Li, L., et al. (2017) · Journal of the American Chemical Society · doi:10.1021/jacs.7b00319 · Cited by 210
Microenvironmental pH and Exosome Levels Interplay in Human Cancer Cell Lines of Different Histotypes
Logozzi, M., Mizzoni, D., Angelini, D. F., Raimo, R. D., Falchi, M., Battistini, L., & Fais, S. (2018) · Cancers · doi:10.3390/cancers10100370 · Cited by 212
A combined “eat me/don't eat me” strategy based on extracellular vesicles for anticancer nanomedicine
Belhadj, Z., He, B., Deng, H., Song, S., Zhang, H., Wang, X., Dai, W., & Zhang, Q. (2020) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2020.1806444 · Cited by 212
Increased Circulating Exosomal miRNA-223 Is Associated with Acute Ischemic Stroke
Chen, Y., Song, Y., Huang, J., Qu, M., Zhang, Y., Geng, J., Zhang, Z., Liu, J., & Yang, G.-Y. (2017) · Frontiers in Neurology · doi:10.3389/fneur.2017.00057 · Cited by 207
Exosomes in developmental signalling
McGough, I. J., & Vincent, J. (2016) · Development · doi:10.1242/dev.126516 · Cited by 217
Electrochemical Sandwich Immunosensor for Determination of Exosomes Based on Surface Marker-Mediated Signal Amplification
Doldán, X., Fagúndez, P., Cayota, A., Laíz, J., & Tosar, J. P. (2016) · Analytical Chemistry · doi:10.1021/acs.analchem.6b02421 · Cited by 207
Proteoglycans remodeling in cancer: Underlying molecular mechanisms
Theocharis, A. D., & Karamanos, N. K. (2017) · Matrix Biology · doi:10.1016/j.matbio.2017.10.008 · Cited by 205
Sensitive Detection of Exosomal Proteins via a Compact Surface Plasmon Resonance Biosensor for Cancer Diagnosis
Liu, C., Zeng, X. R., an, Z., Yang, Y., Eisenbaum, M., Gu, X., Jornet, J. M., Dy, G. K., Reid, M. E., Gan, Q., et al. (2018) · ACS Sensors · doi:10.1021/acssensors.8b00230 · Cited by 204
Tumor-Associated Macrophages in Pancreatic Ductal Adenocarcinoma: Origin, Polarization, Function, and Reprogramming
Yang, S., Liu, Q., & Liao, Q. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.607209 · Cited by 212
Targeted therapy using engineered extracellular vesicles: principles and strategies for membrane modification
Liu, Q., Li, D., Pan, X., & Liang, Y. (2023) · Journal of Nanobiotechnology · doi:10.1186/s12951-023-02081-0 · Cited by 201
Exosomal microRNA miR-1246 induces cell motility and invasion through the regulation of DENND2D in oral squamous cell carcinoma
Sakha, S., Muramatsu, T., Ueda, K., & Inazawa, J. (2016) · Scientific Reports · doi:10.1038/srep38750 · Cited by 201
Detection and isolation of circulating exosomes and microvesicles for cancer monitoring and diagnostics using micro-/nano-based devices
Ko, J., Carpenter, E. L., & Issadore, D. (2015) · The Analyst · doi:10.1039/c5an01610j · Cited by 214
Salivary extracellular vesicle-associated miRNAs as potential biomarkers in oral squamous cell carcinoma
Gai, C., Camussi, F., Broccoletti, R., Gambino, A., Cabras, M., Molinaro, L., Carossa, S., Camussi, G., & Arduino, P. G. (2018) · BMC Cancer · doi:10.1186/s12885-018-4364-z · Cited by 203
Mitochondrial protein enriched extracellular vesicles discovered in human melanoma tissues can be detected in patient plasma
Jang, S. C., Crescitelli, R., Cvjetkovic, A., Belgrano, V., Bagge, R. O., Höög, J. L., Sundfeldt, K., Ochiya, T., Kalluri, R., & Lötvall, J. O. (2019) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2019.1635420 · Cited by 201
Self-citation
Exosomes in the hypoxic TME: from release, uptake and biofunctions to clinical applications
He, G., Peng, X., Wei, S., Yang, S., Li, X., Huang, M., Tang, S., Jin, H., Liu, J., Zhang, S., et al. (2022) · Molecular Cancer · doi:10.1186/s12943-021-01440-5 · Cited by 202
Development of exosome surface display technology in living human cells
Stickney, Z., Losacco, J., McDevitt, S., Zhang, Z., & Lü, B. (2016) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2016.02.058 · Cited by 209
Extracellular vesicles are integral and functional components of the extracellular matrix
Rilla, K. J., Mustonen, A., Arasu, U. T., Härkönen, K., Matilainen, J., & Nieminen, P. (2017) · Matrix Biology · doi:10.1016/j.matbio.2017.10.003 · Cited by 207
Plasma exosome miR-196a and miR-1246 are potential indicators of localized pancreatic cancer
Xu, Y.-F., Hannafon, B. N., Zhao, Y. D., Postier, R. G., & Ding, W. (2017) · Oncotarget · doi:10.18632/oncotarget.20332 · Cited by 203
ExoAPP: Exosome-Oriented, Aptamer Nanoprobe-Enabled Surface Proteins Profiling and Detection
Jin, D., Yang, F., Zhang, Y., Liu, L., Zhou, Y., Wang, F., & Zhang, G. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.8b03959 · Cited by 198
Advanced liquid biopsy technologies for circulating biomarker detection
Soda, N., Rehm, B. H. A., Sonar, P. M., Nguyen, N., & Shiddiky, M. J. A. (2019) · Journal of Materials Chemistry B · doi:10.1039/c9tb01490j · Cited by 205
Exosomal PD-L1 Retains Immunosuppressive Activity and is Associated with Gastric Cancer Prognosis
Fan, Y., Che, X., Qu, J., Hou, K., Wen, T., Li, Z., Li, C., Wang, S., Xu, L., Liu, Y., et al. (2019) · Annals of Surgical Oncology · doi:10.1245/s10434-019-07431-7 · Cited by 197
Inclusion Biogenesis, Methods of Isolation and Clinical Application of Human Cellular Exosomes
Tschuschke, M., Kocherova, I., Bryja, A., Mozdziak, P. E., Volponi, A. A., Janowicz, K., Sibiak, R., Piotrowska‐Kempisty, H., Iżycki, D., Bukowska, D., et al. (2020) · Journal of Clinical Medicine · doi:10.3390/jcm9020436 · Cited by 205
Promoter methylation of ADAMTS1 and BNC1 as potential biomarkers for early detection of pancreatic cancer in blood
Aleissa, M. A., Lerner, L., Abdelfatah, E., Shankar, N., Canner, J. K., Hasan, N. M., Yaghoobi, V., Huang, B., Kerner, Z. J., Takaesu, F., et al. (2019) · Clinical Epigenetics · doi:10.1186/s13148-019-0650-0 · Cited by 196
GPC 1 exosome and its regulatory mi RNA s are specific markers for the detection and target therapy of colorectal cancer
Li, J., Chen, Y., Guo, X., Zhou, L., Jia, Z., Peng, Z., Tang, Y., Liu, W., Zhu, B., Wang, L., et al. (2017) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.12941 · Cited by 194
Microfluidics‐based on‐a‐chip systems for isolating and analysing extracellular vesicles
Guo, S., Tao, S., & Dawn, H. (2018) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2018.1508271 · Cited by 206
Exosomes as mediators of immune regulation and immunotherapy in cancer
Kugeratski, F. G., & Kalluri, R. (2020) · FEBS Journal · doi:10.1111/febs.15558 · Cited by 196
Self-citation
Extracellular matrix component signaling in cancer
Multhaupt, H. A. B., Leitinger, B., Gullberg, D., & Couchman, J. (2015) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2015.10.013 · Cited by 197
The emerging roles of tumor-derived exosomes in hematological malignancies
Boyiadzis, M. M., & Whiteside, T. L. (2017) · Leukemia · doi:10.1038/leu.2017.91 · Cited by 199
Tumor-derived exosomes antagonize innate antiviral immunity
Gao, L., Wang, L., Dai, T., Jin, K., Zhang, Z., Wang, S., Xie, F., Fang, P., Yang, B., Huang, H., et al. (2018) · Nature Immunology · doi:10.1038/s41590-017-0043-5 · Cited by 195
The Yin and Yang of exosome isolation methods: conventional practice, microfluidics, and commercial kits
Shirejini, S. Z., & İnci, F. (2021) · Biotechnology Advances · doi:10.1016/j.biotechadv.2021.107814 · Cited by 194
Fibronectin on circulating extracellular vesicles as a liquid biopsy to detect breast cancer
Moon, P., Lee, J., Cho, Y., Lee, S. J., Chae, Y. S., Jung, J. H., Kim, I.-S., Park, H. Y., & Baek, M. (2016) · Oncotarget · doi:10.18632/oncotarget.9561 · Cited by 197
Exosomal proteins as potential markers of tumor diagnosis
Li, A., Zhang, T., Zheng, M., Liu, Y., & Chen, Z. (2017) · Journal of Hematology & Oncology · doi:10.1186/s13045-017-0542-8 · Cited by 193
Delayed Remote Ischemic Preconditioning ConfersRenoprotection against Septic Acute Kidney Injury via Exosomal miR-21
Pan, T., Jia, P., Chen, N., Fang, Y., Liang, Y., Guo, M., & Ding, X. (2019) · Theranostics · doi:10.7150/thno.29832 · Cited by 191
The updated role of exosomal proteins in the diagnosis, prognosis, and treatment of cancer
Wang, X., Huang, J., Chen, W., Li, G., Li, Z., & Lei, J. (2022) · Experimental & Molecular Medicine · doi:10.1038/s12276-022-00855-4 · Cited by 194
Exosome: a significant nano-scale drug delivery carrier
Peng, H., Ji, W., Zhao, R., Yang, J., Lü, Z., Li, Y., & Zhang, X. (2020) · Journal of Materials Chemistry B · doi:10.1039/d0tb01499k · Cited by 192
Identification of Individual Exosome-Like Vesicles by Surface Enhanced Raman Spectroscopy
Stremersch, S., Marro, M., Pinchasik, B., Baatsen, P., Hendrix, A., Smedt, S. C. D., Loza‐Álvarez, P., Skirtach, A. G., Raemdonck, K., & Braeckmans, K. (2016) · Small · doi:10.1002/smll.201600393 · Cited by 189
Exosomal lncRNA ZFAS1 regulates esophageal squamous cell carcinoma cell proliferation, invasion, migration and apoptosis via microRNA-124/STAT3 axis
Li, Z., Qin, X., Bian, W., Li, Y., Shan, B., Yao, Z., & Li, S. (2019) · Journal of Experimental & Clinical Cancer Research · doi:10.1186/s13046-019-1473-8 · Cited by 187
Characterization of brain‐derived extracellular vesicle lipids in Alzheimer's disease
Su, H., Rustam, Y. H., Masters, C. L., Makalic, E., McLean, C., Hill, A. F., Barnham, K. J., Reid, G. E., & Vella, L. J. (2021) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12089 · Cited by 194
Blocking Tumor-Educated MSC Paracrine Activity Halts Osteosarcoma Progression
Baglio, S. R., Lagerweij, T., Pérez-Lanzón, M., Ho, X. D., Léveillé, N., Melo, S. A., Cleton-Jansen, A.-M., Jordanova, E. S., Roncuzzi, L., Greco, M., et al. (2017) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-16-2726 · Cited by 187
Self-citation
The Multiple Roles of Exosomes in Metastasis
Weidle, U. H., Birzele, F., Kollmorgen, G., & Rüger, R. (2017) · Cancer Genomics & Proteomics · doi:10.21873/cgp.20015 · Cited by 187
Exosomes as potential sources of biomarkers in colorectal cancer
Xiao, Y., Zhong, J., Zhong, B., Huang, J., Jiang, L., Jiang, Y., Yuan, J., Sun, J. G., Dai, L., Yang, C., et al. (2020) · Cancer Letters · doi:10.1016/j.canlet.2020.01.033 · Cited by 186
Exosome‐encapsulated microRNAs as circulating biomarkers for breast cancer
Joyce, D. P., Kerin, M. J., & Dwyer, R. M. (2016) · International Journal of Cancer · doi:10.1002/ijc.30179 · Cited by 187
Correlation of plasma exosomal microRNAs with the efficacy of immunotherapy in EGFR/ALK wild-type advanced non-small cell lung cancer
Peng, X., Yu, R., Wu, X., Wu, S., Pi, C., Chen, Z., Zhang, X., Gao, C.-Y., Shao, Y. W., Liu, L., et al. (2020) · Journal for ImmunoTherapy of Cancer · doi:10.1136/jitc-2019-000376 · Cited by 186
Detailed Analysis of Protein Topology of Extracellular Vesicles–Evidence of Unconventional Membrane Protein Orientation
Cvjetkovic, A., Jang, S. C., Konečná, B., Höög, J. L., Sihlbom, C., Lässer, C., & Lötvall, J. O. (2016) · Scientific Reports · doi:10.1038/srep36338 · Cited by 185
RETRACTED: Exosome-Derived miR-130a Activates Angiogenesis in Gastric Cancer by Targeting C-MYB in Vascular Endothelial Cells
Yang, H., Zhang, H., Ge, S., Ning, T., Bai, M., Li, J., Li, S., Sun, W., Deng, T., Zhang, L., et al. (2018) · Molecular Therapy · doi:10.1016/j.ymthe.2018.07.023 · Cited by 182
Retracted
The role of exosomes in liquid biopsy for cancer diagnosis and prognosis prediction
Li, S., Yi, M., Dong, B., Tan, X., Luo, S., & Wu, K. (2020) · International Journal of Cancer · doi:10.1002/ijc.33386 · Cited by 181
Direct Exosome Quantification via Bivalent-Cholesterol-Labeled DNA Anchor for Signal Amplification
He, F., Liu, H., Guo, X., Yin, B., & Ye, B. (2017) · Analytical Chemistry · doi:10.1021/acs.analchem.7b03919 · Cited by 181
Recent Progress on Liquid Biopsy Analysis using Surface-Enhanced Raman Spectroscopy
Zhang, Y., Mi, X., Tan, X., & Xiang, R. (2019) · Theranostics · doi:10.7150/thno.29875 · Cited by 179
Extracellular Vesicles: Satellites of Information Transfer in Cancer and Stem Cell Biology
Desrochers, L. M., Antonyak, M. A., & Cerione, R. A. (2016) · Developmental Cell · doi:10.1016/j.devcel.2016.04.019 · Cited by 180
The ins and outs of microvesicles
Clancy, J. W., Schmidtmann, M., & D’Souza‐Schorey, C. (2021) · FASEB BioAdvances · doi:10.1096/fba.2020-00127 · Cited by 177
A Ratiometric Fluorescent Bioprobe Based on Carbon Dots and Acridone Derivate for Signal Amplification Detection Exosomal microRNA
Xia, Y., Wang, L., Li, J., Chen, X., Lan, J., Yan, A., Lei, Y., Yang, S., Yang, H., & Chen, J. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.8b01143 · Cited by 175
Development of a rapid lateral flow immunoassay test for detection of exosomes previously enriched from cell culture medium and body fluids
Oliveira-Rodríguez, M., López‐Cobo, S., Reyburn, H. T., Costa‐García, A., López‐Martín, S., Yáñez‐Mó, M., Cernuda‐Morollón, E., Paschen, A., Valés‐Gómez, M., & Blanco‐López, M. C. (2016) · Journal of Extracellular Vesicles · doi:10.3402/jev.v5.31803 · Cited by 179
New Approaches to SCLC Therapy: From the Laboratory to the Clinic
Poirier, J. T., George, J., Owonikoko, T. K., Berns, A. J. M., Brambilla, E. M., Byers, L. A., Carbone, D. P., Chen, H. J., Christensen, C. L., Dive, C., et al. (2020) · Journal of Thoracic Oncology · doi:10.1016/j.jtho.2020.01.016 · Cited by 176
Systematic characterization of extracellular vesicle sorting domains and quantification at the single molecule – single vesicle level by fluorescence correlation spectroscopy and single particle imaging
Corso, G., Heusermann, W., Trojer, D., Görgens, A., Steib, E., Voshol, J., Graff, A., Genoud, C., Lee, Y., Hean, J., et al. (2019) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2019.1663043 · Cited by 180
Quantitative detection of exosomal microRNA extracted from human blood based on surface-enhanced Raman scattering
Ma, D., Huang, C., Zheng, J., Tang, J., Li, J., Yang, J., & Yang, R. (2017) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2017.08.062 · Cited by 171
Highly sensitive detection of exosomes by SERS using gold nanostar@Raman reporter@nanoshell structures modified with a bivalent cholesterol-labeled DNA anchor
Tian, Y., Ning, C.-F., He, F., Yin, B., & Ye, B. (2018) · The Analyst · doi:10.1039/c8an01041b · Cited by 172
Isolation and Visible Detection of Tumor-Derived Exosomes from Plasma
Chen, J., Xu, Y., Lü, Y., & Xing, W. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.8b03031 · Cited by 175
Magnetic and Folate Functionalization Enables Rapid Isolation and Enhanced Tumor-Targeting of Cell-Derived Microvesicles
Zhang, W., Yu, Z., Wu, M. Y., Ren, J., Xia, H.-F., Sa, G., Zhu, J., Pang, D., Zhao, Y., & Chen, G. (2016) · ACS Nano · doi:10.1021/acsnano.6b05630 · Cited by 171
A rapid, automated surface protein profiling of single circulating exosomes in human blood
Kibria, G., Ramos, E. K., Lee, K. E., Bedoyan, S., Huang, S., Samaeekia, R., Athman, J. J., Harding, C. V., Lötvall, J. O., Harris, L. N., et al. (2016) · Scientific Reports · doi:10.1038/srep36502 · Cited by 170
Metalloproteinases in extracellular vesicles
Shimoda, M., & Khokha, R. (2017) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2017.05.027 · Cited by 171
Quantification of Exosome Based on a Copper-Mediated Signal Amplification Strategy
He, F., Wang, J., Yin, B., & Ye, B. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.8b01187 · Cited by 169
Role of Exosomes in the Regulation of T-Cell Mediated Immune Responses and in Autoimmune Disease
(2019) · doi:10.3390/cells8020154 · Cited by 169
Roles of Proteoglycans and Glycosaminoglycans in Cancer Development and Progression
Wei, J., Hu, M., Huang, K., Lin, S., & Du, H. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21175983 · Cited by 169
Exosomes and extracellular vesicles: Rethinking the essential values in cancer biology
Yokoi, A., & Ochiya, T. (2021) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2021.03.032 · Cited by 174
Exosomes: Potential Disease Biomarkers and New Therapeutic Targets
Mosquera-Heredia, M. I., Morales, L. C., Vidal, O. M., Barceló, E., Silvera-Redondo, C. A., Velez, J. I., & Garavito, P. (2021) · Biomedicines · doi:10.3390/biomedicines9081061 · Cited by 173
Liquid Biopsies Using Plasma Exosomal Nucleic Acids and Plasma Cell-Free DNA Compared with Clinical Outcomes of Patients with Advanced Cancers
Möhrmann, L., Huang, H. J., Hong, D. S., Tsimberidou, A. M., Fu, S., Piha-Paul, S. A., Subbiah, V., Karp, D. D., Naing, A., Krug, A., et al. (2017) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-17-2007 · Cited by 168
Extracellular vesicles as modulators of wound healing
Cabral, J., Ryan, A. E., Griffin, M. D., & Ritter, T. (2018) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2018.01.018 · Cited by 171
Extracellular Vesicles in Blood: Sources, Effects, and Applications
Alberro, A., Iparraguirre, L., Fernandes, A., & Otaegui, D. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22158163 · Cited by 170
Tumor-Derived Extracellular Vesicles: Multifunctional Entities in the Tumor Microenvironment
Clancy, J. W., & D’Souza‐Schorey, C. (2022) · Annual Review of Pathology Mechanisms of Disease · doi:10.1146/annurev-pathmechdis-031521-022116 · Cited by 172
Endosomal signalling via exosome surface TGFβ‐1
Shelke, G. V., Yin, Y., Jang, S. C., Lässer, C., Wennmalm, S., Hoffmann, H. J., Li, L., Gho, Y. S., Nilsson, J. A., & Lötvall, J. O. (2019) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2019.1650458 · Cited by 175
Biogenesis, Membrane Trafficking, Functions, and Next Generation Nanotherapeutics Medicine of Extracellular Vesicles
Gurunathan, S., Kang, M.-H., Qasim, M., Khan, K., & Kim, J. (2021) · International Journal of Nanomedicine · doi:10.2147/ijn.s310357 · Cited by 175
Exosome levels in human body fluids: A tumor marker by themselves?
Cappello, F., Logozzi, M., Campanella, C., Bavisotto, C. C., Marcilla, A., Properzi, F., & Fais, S. (2016) · European Journal of Pharmaceutical Sciences · doi:10.1016/j.ejps.2016.09.010 · Cited by 171
The potential of liquid biopsies for the early detection of cancer
Heitzer, E., Perakis, S. O., Geigl, J. B., & Speicher, M. R. (2017) · npj Precision Oncology · doi:10.1038/s41698-017-0039-5 · Cited by 170
A signal-amplifiable biochip quantifies extracellular vesicle-associated RNAs for early cancer detection
Hu, J., Sheng, Y., Kwak, K. J., Shi, J., Yu, B., & Lee, L. J. (2017) · Nature Communications · doi:10.1038/s41467-017-01942-1 · Cited by 167
Toward characterizing extracellular vesicles at a single-particle level
Chiang, C.-Y., & Chen, C. (2019) · Journal of Biomedical Science · doi:10.1186/s12929-019-0502-4 · Cited by 172
Integrated SERS-Vertical Flow Biosensor Enabling Multiplexed Quantitative Profiling of Serological Exosomal Proteins in Patients for Accurate Breast Cancer Subtyping
Su, X., Liu, X., Xie, Y., Chen, M., Zheng, C., Zhong, H. P., & Li, M. (2023) · ACS Nano · doi:10.1021/acsnano.3c00449 · Cited by 166
Cancer-associated circulating large extracellular vesicles in cholangiocarcinoma and hepatocellular carcinoma
Julich‐Haertel, H., Urban, S. K., Krawczyk, M. J., Willms, A. G., Jankowski, K., Patkowski, W., Kruk, B., Krasnodębski, M., Ligocka, J., Schwab, R., et al. (2017) · Journal of Hepatology · doi:10.1016/j.jhep.2017.02.024 · Cited by 168
Glypican-1 is enriched in circulating-exosomes in pancreatic cancer and correlates with tumor burden
Frampton, A. E., Prado, M. M., López-Jiménez, E. A., Fajardo-Puerta, A. B., Jawad, Z., Lawton, P., Giovannetti, E., Habib, N., Castellano, L., Stebbing, J., et al. (2018) · Oncotarget · doi:10.18632/oncotarget.24873 · Cited by 171
Label-free microfluidic cell sorting and detection for rapid blood analysis
Lü, N., Tay, H. M., Petchakup, C., He, L., Gong, L., Maw, K. K., Leong, S. Y., Lok, W. W., Ong, H. B., Guo, R., et al. (2023) · Lab on a Chip · doi:10.1039/d2lc00904h · Cited by 173
Current advances in the use of exosomes, liposomes, and bioengineered hybrid nanovesicles in cancer detection and therapy
Mukherjee, A., Bisht, B., Dutta, S., & Paul, M. K. (2022) · Acta Pharmacologica Sinica · doi:10.1038/s41401-022-00902-w · Cited by 167
Tumor-Educated Platelets as Liquid Biopsy in Cancer Patients
Joosse, S. A., & Pantel, K. (2015) · Cancer Cell · doi:10.1016/j.ccell.2015.10.007 · Cited by 163
From structures to functions: insights into exosomes as promising drug delivery vehicles
Ren, J., He, W., Zheng, L., & Duan, H. (2016) · Biomaterials Science · doi:10.1039/c5bm00583c · Cited by 164
A microRNA signature from serum exosomes of patients with glioma as complementary diagnostic biomarker
Santangelo, A., Imbrucè, P., Gardenghi, B., Belli, L., Agushi, R., Tamanini, A., Munari, S., Bossi, A., Scambi, I., Benati, D., et al. (2017) · Journal of Neuro-Oncology · doi:10.1007/s11060-017-2639-x · Cited by 160
Understanding extracellular vesicle diversity – current status
Greening, D. W., & Simpson, R. J. (2018) · Expert Review of Proteomics · doi:10.1080/14789450.2018.1537788 · Cited by 163
Proteomic insights into extracellular vesicle biology – defining exosomes and shed microvesicles
Greening, D. W., Xu, R., Gopal, S. K., Rai, A., & Simpson, R. J. (2016) · Expert Review of Proteomics · doi:10.1080/14789450.2017.1260450 · Cited by 164
Recent advances and challenges in the recovery and purification of cellular exosomes
Ayala‐Mar, S., Donoso‐Quezada, J., Gallo‐Villanueva, R. C., Pérez‐González, V. H., & González‐Valdez, J. (2019) · Electrophoresis · doi:10.1002/elps.201800526 · Cited by 162
Deubiquitinase Activity Profiling Identifies UCHL1 as a Candidate Oncoprotein That Promotes TGFβ-Induced Breast Cancer Metastasis
Liu, S., González‐Prieto, R., Zhang, M., Geurink, P. P., Kooij, R., Iyengar, P. V., van Dinther, M., Bos, E., Zhang, X., Dévédec, S. E. L., et al. (2019) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-19-1373 · Cited by 162
DBAN: An improved dual branch attention network combined with serum Raman spectroscopy for diagnosis of diabetic kidney disease
Chen, X., Chen, C., Tian, X., He, L., Zuo, E., Liu, P., Xue, Y., Yang, J., Chen, C., & Lv, X. (2023) · Talanta · doi:10.1016/j.talanta.2023.125052 · Cited by 159
Artificial Intelligence and Early Detection of Pancreatic Cancer: 2020 Summative Review
Kenner, B., Chari, S. T., Kelsen, D., Klimstra, D. S., Pandol, S. J., Rosenthal, M., Rustgi, A. K., Taylor, J. A., Yala, A., Abul-Husn, N., et al. (2021) · Pancreas · doi:10.1097/mpa.0000000000001762 · Cited by 158
TransformEHR: transformer-based encoder-decoder generative model to enhance prediction of disease outcomes using electronic health records
Yang, Z., Mitra, A., Liu, W., Berlowitz, D., & Yu, H. (2023) · Nature Communications · doi:10.1038/s41467-023-43715-z · Cited by 158
Exosomes: Diagnostic Biomarkers and Therapeutic Delivery Vehicles for Cancer
Jiang, L., Gu, Y., Du, Y., & Liu, J. (2019) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.9b00409 · Cited by 159
Therapeutic potential of CAR-T cell-derived exosomes: a cell-free modality for targeted cancer therapy
Tang, X., Sun, X., Huang, K.-M., Zhang, L., Yang, Z., Zou, D., Wang, B., Warnock, G. L., Dai, L., & Luo, J. D. (2015) · Oncotarget · doi:10.18632/oncotarget.6175 · Cited by 159
Circulating melanoma exosomes as diagnostic and prognosis biomarkers
Alegre, E., Zubiri, L., Perez‐Gracia, J. L., González‐Cao, M., Soria, L., Martín‐Algarra, S., & González, Á. (2015) · Clinica Chimica Acta · doi:10.1016/j.cca.2015.12.031 · Cited by 157
Extracellular vesicles as emerging targets in cancer: Recent development from bench to bedside
Wu, K., Xing, F., Wu, S.-Y., & Watabe, K. (2017) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/j.bbcan.2017.10.001 · Cited by 158
Exosomes play roles in sequential processes of tumor metastasis
Li, K., Chen, Y., Li, A., Tan, C., & Liu, X. (2018) · International Journal of Cancer · doi:10.1002/ijc.31774 · Cited by 161
Multiplexed immunophenotyping of circulating exosomes on nano-engineered ExoProfile chip towards early diagnosis of cancer
Zhang, P., Zhou, X. M., & Zeng, Y. (2019) · Chemical Science · doi:10.1039/c9sc00961b · Cited by 158
Extracellular Vesicle Transportation and Uptake by Recipient Cells: A Critical Process to Regulate Human Diseases
Kwok, Z. H., Wang, C., & Jin, Y. (2021) · Processes · doi:10.3390/pr9020273 · Cited by 172
Multiplexed analysis of EV reveals specific biomarker composition with diagnostic impact
Spitzberg, J. D., Ferguson, S. W., Yang, K. S., Peterson, H. M., Carlson, J., & Weissleder, R. (2023) · Nature Communications · doi:10.1038/s41467-023-36932-z · Cited by 158
Diagnostic and Therapeutic Potential of Exosomes in Cancer: The Beginning of a New Tale?
Mirzaei, H., Sahebkar, A., Jaafari, M. R., Goodarzi, M., & Mirzaei, H. R. (2016) · Journal of Cellular Physiology · doi:10.1002/jcp.25739 · Cited by 155
Liquid biopsy techniques and pancreatic cancer: diagnosis, monitoring, and evaluation
Wang, K., Wang, X., Pan, Q., & Zhao, B. (2023) · Molecular Cancer · doi:10.1186/s12943-023-01870-3 · Cited by 163

Authors: Melo, S. A., Luecke, L. B., Kahlert, C., Fernandez, A. F., Gammon, S. T., Kaye, J., LeBleu, V. S., Mittendorf, E. A., Weitz, J., Rahbari, N., Reissfelder, C., Pilarsky, C., Fraga, M. F., Piwnica-Worms, D., & Kalluri, R.
Affiliations: The University of Texas MD Anderson Cancer Center (US)
Technische Universität Dresden (DE)
Consejo Superior de Investigaciones Científicas (ES)
Corresponding author: Kalluri, Raghu
ORCID: Kahlert, Christoph
Gammon, Seth T.
LeBleu, Valerie S.
Mittendorf, Elizabeth A.
Rahbari, Nuh
Reissfelder, Christoph
Pilarsky, Christian
Fraga, Mario F.
Piwnica-Worms, David
Kalluri, Raghu
Venue: Nature
Publisher: Springer Science and Business Media LLC
Published: 2015-06-24
Volume: 523
Issue: 7559
Pages: 177-182
ISSN: 0028-0836 (ISSN-L)
1476-4687
DOI: 10.1038/nature14581
PMC: PMC4825698
OpenAlex: W1500925570
Cited by: 2,880
References: 63
Citations by year: 2026: 129 · 2025: 200 · 2024: 253 · 2023: 305 · 2022: 277 · 2021: 371 · 2020: 320 · 2019: 271 · 2018: 269 · 2017: 234 · 2016: 195 · 2015: 52
FWCI: 108.75
Type: journal article
Language: en
Update notices: correction (2022-10-05)
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
www.springer.com/tdm (vor)
Locations: Nature (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
RePEc: Research Papers in Economics (submittedVersion)
Consultation of the Doctoral Thesis Database (TESEO) (Ministerio de Educación, Cultura y Deporte) (submittedVersion, cc-by-nc-nd)
Funders: National Institutes of Health (5u24ca126577-05)
National Cancer Institute (P30CA16672, U01CA151925, CA-16672, U24-CA-126577, P50CA094056, 5U24CA126577, R01CA155370, CA 155370, CA 151925, P30CA016672)
National Institute of Diabetes and Digestive and Kidney Diseases (DK 081576, R01DK081576)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer Genomics and Diagnostics
Health Sciences › Medicine › Oncology › Pancreatic and Hepatic Oncology Research
Keywords: glypican-1, tumor-derived exosomes, extracellular vesicles, pancreatic cancer, early cancer detection, circulating exosomes, cell surface proteoglycans, KRAS mutation, non-invasive biomarkers, tumor burden
MeSH terms: Animals, Female, Humans, Male, Pancreatic Neoplasms, Proto-Oncogene Proteins, Biomarkers, Proto-Oncogene Proteins p21(ras), ras Proteins, NIH 3T3 Cells, HCT116 Cells, Cell Line, Tumor, Mice, Glypicans, Exosomes, MCF-7 Cells

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Glypican-1 identifies cancer exosomes and detects early pancreatic cancer

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks