Cited by 201Extracellular vesicles in diseaseNanoparticle-Based Drug DeliveryRNA Interference and Gene Delivery

Targeted therapy using engineered extracellular vesicles: principles and strategies for membrane modification

Open access
Liu, Qisong, Li, Defeng, Pan, Xiaohua, Liang, Yujie·2023·Journal of Nanobiotechnology

Abstract

Extracellular vesicles (EVs) are 30–150 nm membrane-bound vesicles naturally secreted by cells and play important roles in intercellular communication by delivering regulatory molecules such as proteins, lipids, nucleic acids and metabolites to recipient cells. As natural nano-carriers, EVs possess desirable properties such as high biocompatibility, biological barrier permeability, low toxicity, and low immunogenicity, making them potential therapeutic delivery vehicles. EVs derived from specific cells have inherent targeting capacity towards specific cell types, which is yet not satisfactory enough for targeted therapy development and needs to be improved. Surface modifications endow EVs with targeting abilities, significantly improving their therapeutic efficiency. Herein, we first briefly introduce the biogenesis, composition, uptake and function of EVs, and review the cargo loading approaches for EVs. Then, we summarize the recent advances in surface engineering strategies of EVs, focusing on the applications of engineered EVs for targeted therapy. Altogether, EVs hold great promise for targeted delivery of various cargos, and targeted modifications show promising effects on multiple diseases. Graphical Abstract

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  • Exosomes can be genetically engineered, chemically surface modified or enzymatically engineered to introduce peptides and proteins to enhance their targeting ability.

    “In addition, exosomes can be genetically engineered, chemically surface modified or enzymatically engineered to introduce peptides and proteins to enhance their targeting ability [42, 83–86].”

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Authors
Liu, Q., Li, D., Pan, X., & Liang, Y.
Affiliations
  • Shenzhen University (CN)
  • Jinan University (CN)
Corresponding author
  • Pan, Xiaohua
  • Liang, Yujie
Venue
Journal of Nanobiotechnology
Published
2023-09-16
Volume
21
Issue
1
Pages
334-334
ISSN
1477-3155 (ISSN-L)
OpenAlex
W4386799292
Cited by
201
References
172
Citations by year
2026: 96 · 2025: 65 · 2024: 36 · 2023: 4
FWCI
5.30
Type
journal article
Language
en
Open access
Locations
  • Journal of Nanobiotechnology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Sanming Project of Medicine in Shenzhen (SZGSP013, JSGG20191129094218565, SZSM202106019, SZXK042, GJHZ20190820115203714)
  • Basic and Applied Basic Research Foundation of Guangdong Province (2023A1515011936)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Keywords
extracellular vesicles, engineered extracellular vesicles, membrane modification, surface engineering, targeted delivery, cargo loading, EV biogenesis, EV uptake, intercellular communication, therapeutic delivery vehicles, nanocarriers
MeSH terms
Extracellular Vesicles, Biological Transport, Cell Communication, Nucleic Acids, Permeability

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