Cited by 2,880Extracellular vesicles in diseaseCancer Genomics and DiagnosticsPancreatic and Hepatic Oncology Research

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Glypican-1 identifies cancer exosomes and detects early pancreatic cancer

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Melo, Sonia A., Luecke, Linda B., Kahlert, Christoph, Fernandez, Agustin F., Gammon, Seth T., Kaye, Judith, LeBleu, Valerie S., Mittendorf, Elizabeth A., Weitz, Juergen, Rahbari, Nuh, Reissfelder, Christoph, Pilarsky, Christian, Fraga, Mario F., Piwnica-Worms, David, Kalluri, Raghu·2015·Nature

DOI 10.1038/nature14581PMC PMC4825698

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Abstract

Exosomes are lipid-bilayer-enclosed extracellular vesicles that contain proteins and nucleic acids. They are secreted by all cells and circulate in the blood. Specific detection and isolation of cancer-cell-derived exosomes in the circulation is currently lacking. Using mass spectrometry analyses, we identify a cell surface proteoglycan, glypican-1 (GPC1), specifically enriched on cancer-cell-derived exosomes. GPC1(+) circulating exosomes (crExos) were monitored and isolated using flow cytometry from the serum of patients and mice with cancer. GPC1(+) crExos were detected in the serum of patients with pancreatic cancer with absolute specificity and sensitivity, distinguishing healthy subjects and patients with a benign pancreatic disease from patients with early- and late-stage pancreatic cancer. Levels of GPC1(+) crExos correlate with tumour burden and the survival of pre- and post-surgical patients. GPC1(+) crExos from patients and from mice with spontaneous pancreatic tumours carry specific KRAS mutations, and reliably detect pancreatic intraepithelial lesions in mice despite negative signals by magnetic resonance imaging. GPC1(+) crExos may serve as a potential non-invasive diagnostic and screening tool to detect early stages of pancreatic cancer to facilitate possible curative surgical therapy.

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RETRACTED: MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer
Taylor, D. D., & Gerçel-Taylor, Ç. (2008) · Gynecologic Oncology · doi:10.1016/j.ygyno.2008.04.033 · Cited by 2,333
Retracted
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860
Comparing the Areas under Two or More Correlated Receiver Operating Characteristic Curves: A Nonparametric Approach
DeLong, E. R., DeLong, D. M., & Clarke-Pearson, D. L. (1988) · Biometrics · doi:10.2307/2531595 · Cited by 22,649
Pancreatic Cancer
Spencer, P. M., Cameron, J. L., & Lin, L. (2000) · Cited by 7,202
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Théry, C., Amigorena, S., Raposo, G., & Clayton, A. (2006) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb0322s30 · Cited by 5,766
Exosomes: composition, biogenesis and function
Théry, C., Zitvogel, L., & Amigorena, S. (2002) · Nature reviews. Immunology · doi:10.1038/nri855 · Cited by 5,355
Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers
Skog, J., Würdinger, T., van Rijn, S., Meijer, D. H., Gainche, L., Curry, W. T., Carter, B. S., Krichevsky, A. M., & Breakefield, X. O. (2008) · Nature Cell Biology · doi:10.1038/ncb1800 · Cited by 5,001
Membrane vesicles as conveyors of immune responses
Théry, C., Ostrowski, M., & Segura, É. (2009) · Nature reviews. Immunology · doi:10.1038/nri2567 · Cited by 3,996
Mutation of the mouse klotho gene leads to a syndrome resembling ageing
Kuro-o, M., Matsumura, Y., Aizawa, H., Kawaguchi, H., Suga, T., Utsugi, T., Ohyama, Y., Kurabayashi, M., Kaname, T., Kume, E., et al. (1997) · Nature · doi:10.1038/36285 · Cited by 3,837
Ceramide Triggers Budding of Exosome Vesicles into Multivesicular Endosomes
Trajković, K., Hsu, C., Chiantia, S., Rajendran, L., Wenzel, D., Wieland, F., Schwille, P., Brügger, B., & Simons, M. (2008) · Science · doi:10.1126/science.1153124 · Cited by 3,760

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Exosomal zinc transporter ZIP4 promotes cancer growth and is a novel diagnostic biomarker for pancreatic cancer
Jin, H., Liu, P., Wu, Y., Meng, X., Wu, M., Han, J., & Tan, X. (2018) · Cancer Science · doi:10.1111/cas.13737 · Cited by 158
Diagnosis by extracellular vesicles
Théry, C. (2015) · Nature · doi:10.1038/nature14626 · Cited by 153
Systematic comparison of exosomal proteomes from human saliva and serum for the detection of lung cancer
Sun, Y., Liu, S., Qiao, Z., Shang, Z., Xia, Z., Niu, X., Qian, L., Zhang, Y., Fan, L.-Y., Cao, C., et al. (2017) · Analytica Chimica Acta · doi:10.1016/j.aca.2017.06.005 · Cited by 155
Label-free characterization of exosome via surface enhanced Raman spectroscopy for the early detection of pancreatic cancer
Carmicheal, J., Hayashi, C., Huang, X., Liu, L., Lu, Y., Krasnoslobodtsev, A. V., Lushnikov, A. J., Ps, K., Patel, A., Jain, M., et al. (2018) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2018.11.008 · Cited by 153
Current approaches for avoiding the limitations of circulating tumor cells detection methods—implications for diagnosis and treatment of patients with solid tumors
Kowalik, A., Kowalewska, M., & Góźdź, S. S. (2017) · Translational research · doi:10.1016/j.trsl.2017.04.002 · Cited by 156
Exosomes: New players in cancer
Guo, W., Gao, Y., Li, N., Shao, F., Wang, C., Wang, P., Yang, Z., Li, R., & He, J. (2017) · Oncology Reports · doi:10.3892/or.2017.5714 · Cited by 155
The Glycocalyx and Its Role in Vascular Physiology and Vascular Related Diseases
Weinbaum, S., Cancel, L. M., Fu, B. M., & Tarbell, J. M. (2020) · Cardiovascular Engineering and Technology · doi:10.1007/s13239-020-00485-9 · Cited by 153
MicroRNAs in extracellular vesicles: potential cancer biomarkers
Kinoshita, T., Yip, K. W., Spence, T., & Liu, F. (2016) · Journal of Human Genetics · doi:10.1038/jhg.2016.87 · Cited by 152
Emerging Nanotechnologies for Liquid Biopsy: The Detection of Circulating Tumor Cells and Extracellular Vesicles
Li, W., Wang, H., Zhao, Z., Gao, H., Liu, C., Zhu, L., Wang, C., & Yang, Y. (2018) · Advanced Materials · doi:10.1002/adma.201805344 · Cited by 152
Exosome detection via surface-enhanced Raman spectroscopy for cancer diagnosis
Li, J., Li, Y., Li, P., Zhang, Y., Du, L., Wang, Y., Zhang, C., & Wang, C. (2022) · Acta Biomaterialia · doi:10.1016/j.actbio.2022.03.036 · Cited by 149
Mesenchymal stem/stromal cell extracellular vesicles: From active principle to next generation drug delivery system
Crivelli, B., Chlapanidas, T., Perteghella, S., Lucarelli, E., Pascucci, L., Brini, A. T., Ferrero, I., Marazzi, M., Pessina, A., & Torre, M. L. (2017) · Journal of Controlled Release · doi:10.1016/j.jconrel.2017.07.023 · Cited by 153
Serum exosomal micro RNA s combined with alpha‐fetoprotein as diagnostic markers of hepatocellular carcinoma
Wang, Y., Zhang, C., Zhang, P., Guo, G., Jiang, T., Zhao, X., Jiang, J., Huang, X., Tong, H., & Tian, Y. (2018) · Cancer Medicine · doi:10.1002/cam4.1390 · Cited by 148
Exosomal miR‐423‐5p targets SUFU to promote cancer growth and metastasis and serves as a novel marker for gastric cancer
Yang, H., Fu, H., Wang, B., Zhang, X., Mao, J., Li, X., Wang, M., Sun, Z., Qian, H., & Xu, W. (2018) · Molecular Carcinogenesis · doi:10.1002/mc.22838 · Cited by 148
Recent Progress in Isolation and Detection of Extracellular Vesicles for Cancer Diagnostics
Wang, W., Luo, J., & Wang, S. (2018) · Advanced Healthcare Materials · doi:10.1002/adhm.201800484 · Cited by 151
Imaging and Intracellular Tracking of Cancer-Derived Exosomes Using Single-Molecule Localization-Based Super-Resolution Microscope
Chen, C., Zong, S., Wang, Z., Ju, L., Zhu, D., Zhang, Y., & Cui, Y. (2016) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.6b09442 · Cited by 152
Exosomes, new biomarkers in early cancer detection
Jalalian, S. H., Ramezani, M., Jalalian, S. A., Abnous, K., & Taghdisi, S. M. (2019) · Analytical Biochemistry · doi:10.1016/j.ab.2019.02.013 · Cited by 149
Role of Exosomes and Exosomal microRNAs in Cancer
Dilsiz, N. (2020) · Future Science OA · doi:10.2144/fsoa-2019-0116 · Cited by 149
Exosomes and exosomal RNAs in breast cancer: A status update
Lakshmi, S., Hughes, T. A., & Priya, S. (2020) · European Journal of Cancer · doi:10.1016/j.ejca.2020.11.033 · Cited by 147
Exosomes carrying immunoinhibitory proteins and their role in cancer
Whiteside, T. L. (2017) · Clinical & Experimental Immunology · doi:10.1111/cei.12974 · Cited by 148
Sequential Validation of Blood-Based Protein Biomarker Candidates for Early-Stage Pancreatic Cancer
Capello, M., Bantis, L. E., Scélo, G., Zhao, Y., Peng, L., Dhillon, D. S., Patel, N. J., Kundnani, D. L., Wang, H., Abbruzzese, J. L., et al. (2016) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/djw266 · Cited by 148
Emerging Role of MicroRNAs as Liquid Biopsy Biomarkers in Gastrointestinal Cancers
Shigeyasu, K., Toden, S., Zumwalt, T. J., Okugawa, Y., & Goel, A. (2017) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-16-1676 · Cited by 151
A circulating extracellular vesicles‐based novel screening tool for colorectal cancer revealed by shotgun and data‐independent acquisition mass spectrometry
Zheng, X. E., Xu, K., Zhou, B., Chen, T., Huang, Y., Li, Q., Wen, F., Ge, W., Wang, J., Yu, S., et al. (2020) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2020.1750202 · Cited by 145
Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Lima, L. G., Ham, S., Shin, H., Chai, E. Z. P., Lek, E. S. H., Lobb, R. J., Mueller, A., Mathivanan, S., Yeo, B. J., Choi, Y., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-23946-8 · Cited by 149
Extracellular vesicles as a novel source of biomarkers in liquid biopsies for monitoring cancer progression and drug resistance
Vasconcelos, M. H., Caires, H. R., Ābols, A., Xavier, C. P. R., & Linē, A. (2019) · Drug Resistance Updates · doi:10.1016/j.drup.2019.100647 · Cited by 150
Recent Progress in Detection and Profiling of Cancer Cell‐Derived Exosomes
Xiong, H., Huang, Z., Yang, Z., Lin, Q., Yang, B., Fang, X., Liu, B., Chen, H., & Kong, J. (2021) · Small · doi:10.1002/smll.202007971 · Cited by 144
Biomarkers in the diagnosis of pancreatic cancer: Are we closer to finding the golden ticket?
O'Neill, R. S., & Stoita, A. (2021) · World Journal of Gastroenterology · doi:10.3748/wjg.v27.i26.4045 · Cited by 144
Circulating exosomal CPNE3 as a diagnostic and prognostic biomarker for colorectal cancer
Sun, B., Li, Y., Zhou, Y., Ng, T. K., Zhao, C., Gan, Q., Gu, X., & Xiang, J. (2018) · Journal of Cellular Physiology · doi:10.1002/jcp.26936 · Cited by 144
3D Cellular Architecture Affects MicroRNA and Protein Cargo of Extracellular Vesicles
Rocha, S., Carvalho, J., Oliveira, P., Voglstätter, M., Schvartz, D., Thomsen, A. R., Walter, N., Khanduri, R., Sanchez, J., Keller, A., et al. (2018) · Advanced Science · doi:10.1002/advs.201800948 · Cited by 148
Targets, pitfalls and reference materials for liquid biopsy tests in cancer diagnostics
Geeurickx, E., & Hendrix, A. (2019) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2019.10.005 · Cited by 150
Proteomic dissection of large extracellular vesicle surfaceome unravels interactive surface platform
Rai, A., Fang, H., Claridge, B., Simpson, R. J., & Greening, D. W. (2021) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12164 · Cited by 144
Saliva Diagnostics
NONAKA, T., & Wong, D. T. (2022) · Annual Review of Analytical Chemistry · doi:10.1146/annurev-anchem-061020-123959 · Cited by 145
Exosomes derived from mesenchymal non-small cell lung cancer cells promote chemoresistance
Lobb, R. J., van Amerongen, R. A., Wiegmans, A. P., Ham, S., Larsen, J. E., & Möller, A. (2017) · International Journal of Cancer · doi:10.1002/ijc.30752 · Cited by 144
Extracellular vesicles swarm the cancer microenvironment: from tumor–stroma communication to drug intervention
Wendler, F., Favicchio, R., Simon, T. R., Alifrangis, C., Stebbing, J., & Giamas, G. (2016) · Oncogene · doi:10.1038/onc.2016.253 · Cited by 148
Serum exosomal miR-125b is a novel prognostic marker for hepatocellular carcinoma
Liu, W., Hu, J., Zhou, K., Chen, F., Wang, Z., Liao, B., Dai, Z., Cao, Y., Fan, J., & Zhou, J. (2017) · OncoTargets and Therapy · doi:10.2147/ott.s140062 · Cited by 141
Electrical and Label-Free Quantification of Exosomes with a Reduced Graphene Oxide Field Effect Transistor Biosensor
Yu, Y., Li, Y., Jin, D., Yang, F., Wu, D. C., Xiao, M., Zhang, H., Zhang, Z., & Zhang, G. (2019) · Analytical Chemistry · doi:10.1021/acs.analchem.9b01950 · Cited by 141
Acoustic Enrichment of Extracellular Vesicles from Biological Fluids
Ku, A. T., Lim, H. C., Evander, M., Lilja, H. G., Laurell, T., Scheding, S., & Ceder, Y. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.8b00914 · Cited by 143
Exploiting Exosomes in Cancer Liquid Biopsies and Drug Delivery
Fitts, C. A., Ji, N., Li, Y., & Tan, C. (2019) · Advanced Healthcare Materials · doi:10.1002/adhm.201801268 · Cited by 147
Exosomal biomarkers for cancer diagnosis and patient monitoring
Makler, A., & Asghar, W. (2020) · Expert Review of Molecular Diagnostics · doi:10.1080/14737159.2020.1731308 · Cited by 143
Liquid Biopsies, Novel Approaches and Future Directions
Armakolas, A., Kotsari, M., & Koskinas, J. S. (2023) · Cancers · doi:10.3390/cancers15051579 · Cited by 141
Extracellular vesicles arising from apoptosis: forms, functions, and applications
Gregory, C. D., & Rimmer, M. P. (2023) · The Journal of Pathology · doi:10.1002/path.6138 · Cited by 149
Absolute sizing and label-free identification of extracellular vesicles by flow cytometry
van der Pol, E., de Rond, L., Coumans, F. A. W., Gool, E. L., Böing, A. N., Sturk, A., Nieuwland, R., & van Leeuwen, T. G. J. M. (2018) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2017.12.012 · Cited by 142
DNase I enzyme-aided fluorescence signal amplification based on graphene oxide-DNA aptamer interactions for colorectal cancer exosome detection
Wang, H., Chen, H., Huang, Z., Li, T., Deng, A., & Kong, J. (2018) · Talanta · doi:10.1016/j.talanta.2018.02.083 · Cited by 139
Recent Advances in Glycoproteomic Analysis by Mass Spectrometry
Suttapitugsakul, S., Sun, F., & Wu, R. (2019) · Analytical Chemistry · doi:10.1021/acs.analchem.9b04651 · Cited by 139
Oral Administration of Bovine Milk‐Derived Extracellular Vesicles Alters the Gut Microbiota and Enhances Intestinal Immunity in Mice
Tong, L., Hao, H., Zhang, X., Zhang, Z., Lv, Y., Zhang, L., & Yi, H. (2020) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.201901251 · Cited by 139
Exosome-Mediated Immunosuppression in Tumor Microenvironments
Xie, Q., Zheng, J.-Q., Ding, J., Wu, Y.-F., Liu, L., Yu, Z., & Chen, G. (2022) · Cells · doi:10.3390/cells11121946 · Cited by 144
Application of liquid biopsy in precision medicine: opportunities and challenges
Wang, J., Chang, S., Li, G., & Sun, Y. (2017) · Frontiers of Medicine · doi:10.1007/s11684-017-0526-7 · Cited by 140
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Wang, X., Shen, H., Zhangyuan, G., Huang, R., Zhang, W., He, Q., Jin, K., Zhuo, H., Zhang, Z., Wang, J., et al. (2018) · Cell Death and Disease · doi:10.1038/s41419-017-0180-7 · Cited by 141
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Xiao, Y., Zheng, L., Zou, X., Wang, J., Zhong, J., & Zhong, T. (2019) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2019.1625677 · Cited by 142
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Li, H. R., Liao, Y., Gao, L., Zhuang, T., Huang, Z., Zhu, H., & Ge, J. (2018) · Theranostics · doi:10.7150/thno.21895 · Cited by 137
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Kimura, H., Yamamoto, H., Harada, T., Fumoto, K., Osugi, Y., Sada, R., Maehara, N., Hikita, H., Mori, S., Eguchi, H., et al. (2019) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-18-2124 · Cited by 138
Designer exosomes enabling tumor targeted efficient chemo/gene/photothermal therapy
Wang, J., Chen, P., Dong, Y., Xie, H., Wang, Y., Soto, F., Ma, P., Feng, X., Du, W., & Liu, B. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2021.121056 · Cited by 136
Extracellular vesicles and particles impact the systemic landscape of cancer
Lucotti, S., Kenific, C. M., Zhang, H., & Lyden, D. C. (2022) · The EMBO Journal · doi:10.15252/embj.2021109288 · Cited by 145
Elevated CEA and CA19-9 serum levels independently predict advanced pancreatic cancer at diagnosis
van Manen, L., Groen, J. V., Putter, H., Vahrmeijer, A. L., Swijnenburg, R., Bonsing, B. A., & Mieog, J. S. D. (2020) · Biomarkers · doi:10.1080/1354750x.2020.1725786 · Cited by 133
PDX-derived organoids model in vivo drug response and secrete biomarkers
Huang, L., Bockorny, B., Paul, I., Akshinthala, D., Frappart, P., Gandarilla, O., Bose, A., Sanchez‐Gonzalez, V., Rouse, E. E., Lehoux, S. D., et al. (2020) · JCI Insight · doi:10.1172/jci.insight.135544 · Cited by 138
Tumor-derived small extracellular vesicles in cancer invasion and metastasis: molecular mechanisms, and clinical significance
Zhang, C., Qin, C., Dewanjee, S., Bhattacharya, H., Chakraborty, P., Jha, N. K., Gangopadhyay, M., Jha, S. K., & Liu, Q. (2024) · Molecular Cancer · doi:10.1186/s12943-024-01932-0 · Cited by 134
Screening for Pancreatic Adenocarcinoma Using Signals From Web Search Logs: Feasibility Study and Results
Paparrizos, J., White, R. W., & Horvitz, E. (2016) · Journal of Oncology Practice · doi:10.1200/jop.2015.010504 · Cited by 132
The mini player with diverse functions: extracellular vesicles in cell biology, disease, and therapeutics
Thakur, A., Ke, X., Chen, Y., Motallebnejad, P., Zhang, K., Lian, Q., & Chen, H. J. (2021) · Protein & Cell · doi:10.1007/s13238-021-00863-6 · Cited by 138
Detection of phosphatidylserine-positive exosomes for the diagnosis of early-stage malignancies
Sharma, R., Huang, X., Brekken, R. A., & Schroit, A. J. (2017) · British Journal of Cancer · doi:10.1038/bjc.2017.183 · Cited by 133
Detection of exosomes by ZnO nanowires coated three-dimensional scaffold chip device
Chen, Z. B., Cheng, S., Cao, P., Qiu, Q., Chen, Y., Xie, M., Yu, X., & Huang, W. (2018) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2018.09.033 · Cited by 133
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Jaiswal, R., & Sedger, L. M. (2019) · Frontiers in Oncology · doi:10.3389/fonc.2019.00125 · Cited by 140
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Buscail, É., Alix‐Panabières, C., Quincy, P., Cauvin, T., Chauvet, A., Degrandi, O., Caumont, C., Verdon, S., Lamrissi‐Garcia, I., Moranvillier, I., et al. (2019) · Cancers · doi:10.3390/cancers11111656 · Cited by 138
Recent advances of aptasensors for exosomes detection
Zhu, C., Li, L., Wang, Z., Irfan, M., & Qu, F. (2020) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2020.112213 · Cited by 133
Small extracellular vesicles in cancer
Abhange, K. K., Makler, A., Wen, Y., Ramnauth, N., Mao, W., Asghar, W., & Wan, Y. (2021) · Bioactive Materials · doi:10.1016/j.bioactmat.2021.03.015 · Cited by 139
Elevated levels of extracellular vesicles are associated with therapy failure and disease progression in breast cancer patients undergoing neoadjuvant chemotherapy
König, L., Kasimir‐Bauer, S., Bittner, A., Hoffmann, O., Wagner, B., Manvailer, L. F. S., Kimmig, R., Horn, P. A., & Rebmann, V. (2017) · OncoImmunology · doi:10.1080/2162402x.2017.1376153 · Cited by 136
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Zhang, P., Zhou, H., Lu, K., Lu, Y., Wang, Y., & Feng, T. (2018) · OncoTargets and Therapy · doi:10.2147/ott.s155134 · Cited by 132
Exosomal TRIM3 is a novel marker and therapy target for gastric cancer
Fu, H., Yang, H., Zhang, X., Wang, B., Mao, J., Li, X., Wang, M., Zhang, B., Sun, Z., Qian, H., et al. (2018) · Journal of Experimental & Clinical Cancer Research · doi:10.1186/s13046-018-0825-0 · Cited by 131
Exosomes: Isolation, Analysis, and Applications in Cancer Detection and Therapy
Bu, H., He, D., He, X., & Wang, K. (2018) · ChemBioChem · doi:10.1002/cbic.201800470 · Cited by 129
Synergies in exosomes and autophagy pathways for cellular homeostasis and metastasis of tumor cells
Salimi, L., Akbari, A., Jabbari, N., Mojarad, B., Vahhabi, A., Szafert, S., Kalashani, S. A., Soraya, H., Nawaz, M., & Rezaie, J. (2020) · Cell & Bioscience · doi:10.1186/s13578-020-00426-y · Cited by 130
Roles of Exosomes in Ocular Diseases
Liu, J., Jiang, F., Jiang, Y., Wang, Y., Li, Z., Shi, X., Zhu, Y., Wang, H., & Zhang, Z. (2020) · International Journal of Nanomedicine · doi:10.2147/ijn.s277190 · Cited by 131
Clinical applications for exosomes: Are we there yet?
Perocheau, D., Touramanidou, L., Gurung, S., Gissen, P., & Baruteau, J. (2021) · British Journal of Pharmacology · doi:10.1111/bph.15432 · Cited by 123
Emerging role of exosomes as biomarkers in cancer treatment and diagnosis
Hanjani, N. A., Esmaelizad, N., Zanganeh, S., Gharavi, A. T., Heidarizadeh, P., Radfar, M., Omidi, F., MacLoughlin, R. J., & Doroudian, M. (2021) · Critical Reviews in Oncology/Hematology · doi:10.1016/j.critrevonc.2021.103565 · Cited by 131
Exosomes—the enigmatic regulators of bone homeostasis
Gao, M., Gao, W., Papadimitriou, J. M., Zhang, C., Gao, J., & Zheng, M. (2018) · Bone Research · doi:10.1038/s41413-018-0039-2 · Cited by 131
RETRACTED: Exosomes Derived from miR-143-Overexpressing MSCs Inhibit Cell Migration and Invasion in Human Prostate Cancer by Downregulating TFF3
Che, Y., Shi, X., Shi, Y., Jiang, X., Ai, Q., Shi, Y., Gong, F., & Jiang, W. (2019) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2019.08.010 · Cited by 128
Retracted
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Sun, Y., Jin, H. Z., Jiang, X., & Gui, R. (2020) · Analytical Chemistry · doi:10.1021/acs.analchem.9b05583 · Cited by 128
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Yang, Z., Lariviere, M. J., Ko, J., Till, J. E., Christensen, T. E., Yee, S. S., Black, T. A., Tien, K., Lin, A. A., Shen, H., et al. (2020) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-19-3313 · Cited by 130
Glycosylation of Cancer Extracellular Vesicles: Capture Strategies, Functional Roles and Potential Clinical Applications
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Exosomes and their role in the micro-/macro-environment: a comprehensive review
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Authors: Melo, S. A., Luecke, L. B., Kahlert, C., Fernandez, A. F., Gammon, S. T., Kaye, J., LeBleu, V. S., Mittendorf, E. A., Weitz, J., Rahbari, N., Reissfelder, C., Pilarsky, C., Fraga, M. F., Piwnica-Worms, D., & Kalluri, R.
Affiliations: The University of Texas MD Anderson Cancer Center (US)
Technische Universität Dresden (DE)
Consejo Superior de Investigaciones Científicas (ES)
Corresponding author: Kalluri, Raghu
ORCID: Kahlert, Christoph
Gammon, Seth T.
LeBleu, Valerie S.
Mittendorf, Elizabeth A.
Rahbari, Nuh
Reissfelder, Christoph
Pilarsky, Christian
Fraga, Mario F.
Piwnica-Worms, David
Kalluri, Raghu
Venue: Nature
Publisher: Springer Science and Business Media LLC
Published: 2015-06-24
Volume: 523
Issue: 7559
Pages: 177-182
ISSN: 0028-0836 (ISSN-L)
1476-4687
DOI: 10.1038/nature14581
PMC: PMC4825698
OpenAlex: W1500925570
Cited by: 2,880
References: 63
Citations by year: 2026: 129 · 2025: 200 · 2024: 253 · 2023: 305 · 2022: 277 · 2021: 371 · 2020: 320 · 2019: 271 · 2018: 269 · 2017: 234 · 2016: 195 · 2015: 52
FWCI: 108.75
Type: journal article
Language: en
Update notices: correction (2022-10-05)
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
www.springer.com/tdm (vor)
Locations: Nature (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
RePEc: Research Papers in Economics (submittedVersion)
Consultation of the Doctoral Thesis Database (TESEO) (Ministerio de Educación, Cultura y Deporte) (submittedVersion, cc-by-nc-nd)
Funders: National Institutes of Health (5u24ca126577-05)
National Cancer Institute (P30CA16672, U01CA151925, CA-16672, U24-CA-126577, P50CA094056, 5U24CA126577, R01CA155370, CA 155370, CA 151925, P30CA016672)
National Institute of Diabetes and Digestive and Kidney Diseases (DK 081576, R01DK081576)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer Genomics and Diagnostics
Health Sciences › Medicine › Oncology › Pancreatic and Hepatic Oncology Research
Keywords: glypican-1, tumor-derived exosomes, extracellular vesicles, pancreatic cancer, early cancer detection, circulating exosomes, cell surface proteoglycans, KRAS mutation, non-invasive biomarkers, tumor burden
MeSH terms: Animals, Female, Humans, Male, Pancreatic Neoplasms, Proto-Oncogene Proteins, Biomarkers, Proto-Oncogene Proteins p21(ras), ras Proteins, NIH 3T3 Cells, HCT116 Cells, Cell Line, Tumor, Mice, Glypicans, Exosomes, MCF-7 Cells

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Glypican-1 identifies cancer exosomes and detects early pancreatic cancer

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