The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Operation of a homeostatic sleep switch
Open accessAbstract
Sleep disconnects animals from the external world, at considerable risks and costs that must be offset by a vital benefit. Insight into this mysterious benefit will come from understanding sleep homeostasis: to monitor sleep need, an internal bookkeeper must track physiological changes that are linked to the core function of sleep. In Drosophila, a crucial component of the machinery for sleep homeostasis is a cluster of neurons innervating the dorsal fan-shaped body (dFB) of the central complex. Artificial activation of these cells induces sleep, whereas reductions in excitability cause insomnia. dFB neurons in sleep-deprived flies tend to be electrically active, with high input resistances and long membrane time constants, while neurons in rested flies tend to be electrically silent. Correlative evidence thus supports the simple view that homeostatic sleep control works by switching sleep-promoting neurons between active and quiescent states. Here we demonstrate state switching by dFB neurons, identify dopamine as a neuromodulator that operates the switch, and delineate the switching mechanism. Arousing dopamine caused transient hyperpolarization of dFB neurons within tens of milliseconds and lasting excitability suppression within minutes. Both effects were transduced by Dop1R2 receptors and mediated by potassium conductances. The switch to electrical silence involved the downregulation of voltage-gated A-type currents carried by Shaker and Shab, and the upregulation of voltage-independent leak currents through a two-pore-domain potassium channel that we term Sandman. Sandman is encoded by the CG8713 gene and translocates to the plasma membrane in response to dopamine. dFB-restricted interference with the expression of Shaker or Sandman decreased or increased sleep, respectively, by slowing the repetitive discharge of dFB neurons in the ON state or blocking their entry into the OFF state. Biophysical changes in a small population of neurons are thus linked to the control of sleep–wake state.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Independent optical excitation of distinct neural populations
Klapoetke, N. C., Murata, Y., Kim, S. S., Pulver, S. R., Birdsey-Benson, A., Cho, Y. K., Morimoto, T. K., Chuong, A. S., Carpenter, E. J., Tian, Z., et al. (2014) · Nature Methods · doi:10.1038/nmeth.2836 · Cited by 2,344
A GAL4-Driver Line Resource for Drosophila Neurobiology
Jenett, A., Rubin, G. M., Ngo, T.-T. B., Shepherd, D., Murphy, C., Dionne, H., Pfeiffer, B. D., Cavallaro, A., Hall, D., Jeter, J., et al. (2012) · Cell Reports · doi:10.1016/j.celrep.2012.09.011 · Cited by 1,449
Dopaminergic modulation of neuronal excitability in the striatum and nucleus accumbens
Nicola, S. M., Surmeier, D. J., & Malenka, R. C. (2000) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.23.1.185 · Cited by 931
Cited by
A connectome and analysis of the adult Drosophila central brain
Scheffer, L. K., Xu, C. S., Januszewski, M., Lu, Z., Takemura, S.-Y., Hayworth, K. J., Huang, G. B., Shinomiya, K., Maitlin-Shepard, J., Berg, S., et al. (2020) · eLife · doi:10.7554/elife.57443 · Cited by 1,128
“Some neurons even switch between graded and spiking operation (Pimentel et al., 2016).”
BackgroundNeural Circuitry of Wakefulness and Sleep
Scammell, T. E., Arrigoni, E., & Lipton, J. O. (2017) · Neuron · doi:10.1016/j.neuron.2017.01.014 · Cited by 966
VTA dopaminergic neurons regulate ethologically relevant sleep-wake behaviors
Eban-Rothschild, A., Rothschild, G., Giardino, W. J., Jones, J. R., & de Lecea, L. (2016) · Nature Neuroscience · doi:10.1038/nn.4377 · Cited by 583
Hulse, B. K., Haberkern, H., Franconville, R., Turner-Evans, D., Takemura, S.-Y., Wolff, T., Noorman, M., Dreher, M., Dan, C., Parekh, R., et al. (2021) · eLife · doi:10.7554/elife.66039 · Cited by 392
Chemoconnectomics: Mapping Chemical Transmission in Drosophila
Deng, B., Li, Q., Liu, X., Cao, Y., Li, B., Qian, Y., Xu, R., Mao, R., Zhou, E., Zhang, W., et al. (2019) · Neuron · doi:10.1016/j.neuron.2019.01.045 · Cited by 340
Dorsal Raphe Dopamine Neurons Modulate Arousal and Promote Wakefulness by Salient Stimuli
Cho, J. R., Treweek, J. B., Robinson, J. E., Xiao, C., Bremner, L. R., Greenbaum, A., & Gradinaru, V. (2017) · Neuron · doi:10.1016/j.neuron.2017.05.020 · Cited by 276
Neuronal Mechanisms for Sleep/Wake Regulation and Modulatory Drive
Eban-Rothschild, A., Appelbaum, L., & de Lecea, L. (2018) · Neuropsychopharmacology · doi:10.1038/npp.2017.294 · Cited by 282
Tsao, C.-H., Chen, C.-C., Lin, C.-H., Yang, H.-Y., & Lin, S. (2018) · eLife · doi:10.7554/elife.35264 · Cited by 214
Differential regulation of the Drosophila sleep homeostat by circadian and arousal inputs
Ni, J. D., Gurav, A. S., Liu, W., Ogunmowo, T. H., Hackbart, H., Elsheikh, A., Verdegaal, A. A., & Montell, C. (2019) · eLife · doi:10.7554/elife.40487 · Cited by 93
Ring Attractor Dynamics Emerge from a Spiking Model of the Entire Protocerebral Bridge
Kakaria, K. S., & de Bivort, B. L. (2017) · Frontiers in Behavioral Neuroscience · doi:10.3389/fnbeh.2017.00008 · Cited by 89
Neuronal reactivation during post-learning sleep consolidates long-term memory in Drosophila
Dag, U., Lei, Z., Le, J. Q., Wong, A., Bushey, D., & Keleman, K. (2019) · eLife · doi:10.7554/elife.42786 · Cited by 83
Mitochondrial origins of the pressure to sleep
Sarnataro, R., Velasco, C. D., Monaco, N., Kempf, A., & Miesenböck, G. (2025) · Nature · doi:10.1038/s41586-025-09261-y · Cited by 62
Self-citationNeuronal substrates of sleep homeostasis; lessons from flies, rats and mice
Donlea, J. M., Alam, M. N., & Szymusiak, R. (2017) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2017.05.003 · Cited by 56
Self-citationAsjad, H. M. M., Kasture, A., El-Kasaby, A., Sackel, M., Hummel, T., Freissmuth, M., & Sucic, S. (2017) · Journal of Biological Chemistry · doi:10.1074/jbc.m117.797092 · Cited by 53
Hulse, B. K., Haberkern, H., Franconville, R., Turner-Evans, D. B., Takemura, S., Wolff, T., Noorman, M., Dreher, M., Dan, C., Parekh, R., et al. (2020) · doi:10.1101/2020.12.08.413955 · Cited by 392
“A) Neuron-to-neuron connectivity matrix between R23E10 neurons (marked by blue text) and dopaminergic neurons (marked by magenta text).”
BackgroundInfluentialA rise-to-threshold process for a relative-value decision
Vijayan, V., Wang, F., Wang, K., Chakravorty, A., Adachi, A., Akhlaghpour, H., Dickson, B. J., & Maimon, G. (2023) · Nature · doi:10.1038/s41586-023-06271-6 · Cited by 49
Holst, S. C., Müller, T., Valomon, A., Seebauer, B., Berger, W., & Landolt, H.-P. (2017) · Scientific Reports · doi:10.1038/srep45982 · Cited by 45
Troup, M., Yap, M. H., Rohrscheib, C., Grabowska, M. J., Ertekin, D., Randeniya, R., Kottler, B., Larkin, A., Munro, K., Shaw, P. J., et al. (2018) · eLife · doi:10.7554/elife.37105 · Cited by 43
Re-examining the role of the dorsal fan-shaped body in promoting sleep in Drosophila
De, J., Wu, M., Lambatan, V., Hua, Y., & Joiner, W. J. (2023) · Current Biology · doi:10.1016/j.cub.2023.07.043 · Cited by 42
A connectome is not enough – what is still needed to understand the brain of Drosophila?
Scheffer, L. K., & Meinertzhagen, I. A. (2021) · Journal of Experimental Biology · doi:10.1242/jeb.242740 · Cited by 27
On the role of the head ganglia in posture and walking in insects
Emanuel, S., Kaiser, M., Pflueger, H.-J., & Libersat, F. (2020) · Frontiers in Physiology · doi:10.3389/fphys.2020.00135 · Cited by 31
Metadata
- Authors
- Pimentel, D., Donlea, J. M., Talbot, C. B., Song, S. M., Thurston, A. J. F., & Miesenböck, G.
- Venue
- Nature
- Published
- 2016-08-03
- Volume
- 536
- Issue
- 7616
- Pages
- 333-337
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2481995903
- Cited by
- 265
- References
- 3
- FWCI
- 16.29
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
