Ethanol Synthesis by Genetic Engineering in Cyanobacteria
Open accessAbstract
Cyanobacteria are autotrophic prokaryotes which carry out oxygenic photosynthesis and accumulate glycogen as the major form of stored carbon. In this research, we introduced new genes into a cyanobacterium in order to create a novel pathway for fixed carbon utilization which results in the synthesis of ethanol. The coding sequences of pyruvate decarboxylase ( pdc ) and alcohol dehydrogenase II ( adh ) from the bacterium Zymomonas mobilis were cloned into the shuttle vector pCB4 and then used to transform the cyanobacterium Synechococcus sp. strain PCC 7942. Under control of the promoter from the rbcLS operon encoding the cyanobacterial ribulose-1,5-bisphosphate carboxylase/oxygenase, the pdc and adh genes were expressed at high levels, as demonstrated by Western blotting and enzyme activity analyses. The transformed cyanobacterium synthesized ethanol, which diffused from the cells into the culture medium. As cyanobacteria have simple growth requirements and use light, CO 2 , and inorganic elements efficiently, production of ethanol by cyanobacteria is a potential system for bioconversion of solar energy and CO 2 into a valuable resource.
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The first cyanobacterial species to be genetically modified to produce ethanol was Synechococcus sp. PCC 7942.
“The first cyanobacterial species to be genetically modified in order to produce ethanol was Synechococcus sp. PCC 7942.”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Deng and Coleman 1999
The transformed Synechococcus sp. PCC 7942 strain with pyruvate decarboxylase and alcohol dehydrogenase II from Zymomonas mobilis produced ethanol yields reaching 54 nmol OD730 unit−1 liter−1 day−1.
“The reported yields of ethanol produced by the transformed strain reached 54 nmol OD730 unit−1. liter−1. day−1 (Deng and Coleman 1999).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Deng and Coleman 1999
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- Authors
- Deng, M.-D., & Coleman, J. R.
- Affiliations
- University of Toronto (CA)
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1999-02
- Volume
- 65
- Issue
- 2
- Pages
- 523-528
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC91056
- OpenAlex
- W2156663310
- Cited by
- 457
- References
- 40
- Citations by year
- 2026: 9 · 2025: 13 · 2024: 10 · 2023: 21 · 2022: 21 · 2021: 24 · 2020: 29 · 2019: 37 · 2018: 25 · 2017: 24 · 2016: 44 · 2015: 37 · 2014: 39 · 2013: 31 · 2012: 30
- FWCI
- 0.92
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Keywords
- ethanol production, cyanobacteria, Synechococcus elongatus PCC 7942, pyruvate decarboxylase, Zymomonas mobilis, heterologous expression, CO2 fixation, glycogen utilization
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