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Detecting and characterizing circular RNAs

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Jeck, William R, Sharpless, Norman E·2014·Nature Biotechnology
DOI 10.1038/nbt.2890
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Abstract

Circular RNA transcripts were first identified in the early 1990s but knowledge of these species has remained limited, as their study through traditional methods of RNA analysis has been difficult. Now, novel bioinformatic approaches coupled with biochemical enrichment strategies and deep sequencing have allowed comprehensive studies of circular RNA species. Recent studies have revealed thousands of endogenous circular RNAs in mammalian cells, some of which are highly abundant and evolutionarily conserved. Evidence is emerging that some circRNAs might regulate microRNA (miRNA) function, and roles in transcriptional control have also been suggested. Therefore, study of this class of noncoding RNAs has potential implications for therapeutic and research applications. We believe the key future challenge for the field will be to understand the regulation and function of these unusual molecules.

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Metadata

Authors
Jeck, W. R., & Sharpless, N. E.
Venue
Nature Biotechnology
Publisher
Springer Science and Business Media LLC
Published
2014-05
Volume
32
Issue
5
Pages
453-461
ISSN
  • 0733-222X (ISSN-L)
  • 1087-0156
  • 1546-1696
DOI
10.1038/nbt.2890
OpenAlex
W2017919052
Cited by
2,702
References
8
FWCI
35.53
Type
journal article
Language
en
Open access
green
Licence
www.springer.com/tdm (tdm)

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Jeck, W. R., & Sharpless, N. E. (2014). Detecting and characterizing circular RNAs. Nature Biotechnology. https://doi.org/10.1038/nbt.2890

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