Circular intronic long noncoding RNAs
Open accessAbstract
We describe the identification and characterization of circular intronic long noncoding RNAs in human cells, which accumulate owing to a failure in debranching. The formation of such circular intronic RNAs (ciRNAs) can be recapitulated using expression vectors, and their processing depends on a consensus motif containing a 7 nt GU-rich element near the 5' splice site and an 11 nt C-rich element close to the branchpoint site. In addition, we show that ciRNAs are abundant in the nucleus and have little enrichment for microRNA target sites. Importantly, knockdown of ciRNAs led to the reduced expression of their parent genes. One abundant such RNA, ci-ankrd52, largely accumulates to its sites of transcription, associates with elongation Pol II machinery, and acts as a positive regulator of Pol II transcription. This study thus suggests a cis-regulatory role of noncoding intronic transcripts on their parent coding genes.
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Three types of circRNAs have been reported: circular intronic RNAs (ciRNAs), retained-intron or exon-intron circRNAs (EIciRNAs), and intergenic circRNAs.
“Three types of circRNAs have been reported, which are produced via different molecular processes, including circular intronic RNAs (ciR-NAs), retained-intron or exon-intron circRNAs (EIciRNAs), and intergenic circRNAs [40,41] (Fig. 1).”
From Circular RNAs in depression: Biogenesis, function, expression, and therapeutic potential (2021) · cites this paper as [40]
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- Authors
- Zhang, Y., Zhang, X.-O., Chen, T., Xiang, J.-F., Yin, Q.-F., Xing, Y.-H., Zhu, S., Yang, L., & Chen, L.-L.
- Affiliations
- Chinese Academy of Sciences (CN)
- Corresponding author
- Yang, Li
- Chen, Ling-Ling
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2013-09
- Volume
- 51
- Issue
- 6
- Pages
- 792-806
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W2055493067
- Cited by
- 2,221
- References
- 54
- Citations by year
- 2026: 81 · 2025: 129 · 2024: 185 · 2023: 191 · 2022: 253 · 2021: 345 · 2020: 295 · 2019: 233 · 2018: 212 · 2017: 127 · 2016: 78 · 2015: 58 · 2014: 27 · 2013: 3
- FWCI
- 24.80
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Molecular Cell (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Circular RNAs in diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Keywords
- RNA splicing, branch points, cis-regulation, RNA polymerase II transcription, microRNA binding sites, gene expression regulation
- MeSH terms
- DNA Polymerase II, Humans, Introns, RNA Polymerase II, Transcription, Genetic, RNA Splice Sites, MicroRNAs, Gene Knockdown Techniques, RNA, Long Noncoding
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