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Real-time reliable determination of binding kinetics of DNA hybridization using a multi-channel graphene biosensor

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Xu, Shicai, Zhan, Jian, Man, Baoyuan, Jiang, Shouzhen, Yue, Weiwei, Gao, Shoubao, Guo, Chengang, Liu, Hanping, Li, Zhenhua, Wang, Jihua, Zhou, Yaoqi·2017·Nature Communications

DOI 10.1038/ncomms14902

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Abstract

Reliable determination of binding kinetics and affinity of DNA hybridization and single-base mismatches plays an essential role in systems biology, personalized and precision medicine. The standard tools are optical-based sensors that are difficult to operate in low cost and to miniaturize for high-throughput measurement. Biosensors based on nanowire field-effect transistors have been developed, but reliable and cost-effective fabrication remains a challenge. Here, we demonstrate that a graphene single-crystal domain patterned into multiple channels can measure time- and concentration-dependent DNA hybridization kinetics and affinity reliably and sensitively, with a detection limit of 10 pM for DNA. It can distinguish single-base mutations quantitatively in real time. An analytical model is developed to estimate probe density, efficiency of hybridization and the maximum sensor response. The results suggest a promising future for cost-effective, high-throughput screening of drug candidates, genetic variations and disease biomarkers by using an integrated, miniaturized, all-electrical multiplexed, graphene-based DNA array.

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Authors: Xu, S., Zhan, J., Man, B., Jiang, S., Yue, W., Gao, S., Guo, C., Liu, H., Li, Z., Wang, J., & Zhou, Y.
Venue: Nature Communications
Publisher: Springer Science and Business Media LLC
Published: 2017-03-21
Volume: 8
Issue: 1
Pages: 14902-14902
ISSN: 2041-1723 (ISSN-L)
DOI: 10.1038/ncomms14902
OpenAlex: W2603262868
Cited by: 437
References: 54
FWCI: 16.69
Type: journal article
Language: en
Open access: gold

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Real-time reliable determination of binding kinetics of DNA hybridization using a multi-channel graphene biosensor

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