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Rapid detection of single bacteria in unprocessed blood using Integrated Comprehensive Droplet Digital Detection
Open accessAbstract
Blood stream infection or sepsis is a major health problem worldwide, with extremely high mortality, which is partly due to the inability to rapidly detect and identify bacteria in the early stages of infection. Here we present a new technology termed ‘Integrated Comprehensive Droplet Digital Detection’ (IC 3D) that can selectively detect bacteria directly from milliliters of diluted blood at single-cell sensitivity in a one-step, culture- and amplification-free process within 1.5–4 h. The IC 3D integrates real-time, DNAzyme-based sensors, droplet microencapsulation and a high-throughput 3D particle counter system. Using Escherichia coli as a target, we demonstrate that the IC 3D can provide absolute quantification of both stock and clinical isolates of E. coli in spiked blood within a broad range of extremely low concentration from 1 to 10,000 bacteria per ml with exceptional robustness and limit of detection in the single digit regime.
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Metadata
- Authors
- Kang, D.-K., Ali, M. M., Zhang, K., Huang, S. S., Peterson, E., Digman, M. A., Gratton, E., & Zhao, W.
- Venue
- Nature Communications
- Published
- 2014-11-13
- Volume
- 5
- Issue
- 1
- Pages
- 5427-5427
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2040654803
- Cited by
- 297
- References
- 36
- FWCI
- 13.57
- Type
- journal article
- Language
- en
- Open access
- hybrid
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