The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells
Open accessAbstract
To combat hostile viruses, bacteria and archaea have evolved a unique antiviral defense system composed of clustered regularly interspaced short palindromic repeats (CRISPRs), together with CRISPR-associated genes (Cas). The CRISPR/Cas9 system develops an adaptive immune resistance to foreign plasmids and viruses by creating site-specific DNA double-stranded breaks (DSBs). Here we adapt the CRISPR/Cas9 system to human cells for intracellular defense against foreign DNA and viruses. Using HIV-1 infection as a model, our results demonstrate that the CRISPR/Cas9 system disrupts latently integrated viral genome and provides long-term adaptive defense against new viral infection, expression and replication in human cells. We show that engineered human-induced pluripotent stem cells stably expressing HIV-targeted CRISPR/Cas9 can be efficiently differentiated into HIV reservoir cell types and maintain their resistance to HIV-1 challenge. These results unveil the potential of the CRISPR/Cas9 system as a new therapeutic strategy against viral infections. The CRISPR/Cas9 system can be used for genome editing. Here, Liao et al. show that the system can be adapted to inhibit HIV expression and replication, excise the integrated HIV genome and provide long-term protection against new infections in human cells, including pluripotent stem cells.
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Cites
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“Since then, various research groups have produced abundant data on off-target sites from different systems using different strategies [10–17].”
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Engineering virus resistance via CRISPR–Cas systems
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Self-citationPandey, M., Ojha, D., Bansal, S., Rode, A. B., & Chawla, G. (2021) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2021.101003 · Cited by 23
Metadata
- Authors
- Liao, H.-K., Gu, Y., Diaz, A., Marlett, J., Takahashi, Y., Li, M., Suzuki, K., Xu, R., Hishida, T., Chang, C.-J., Esteban, C. R., Young, J., & Belmonte, J. C. I.
- Venue
- Nature Communications
- Published
- 2015-03-10
- Volume
- 6
- Issue
- 1
- Pages
- 6413-6413
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2049490177
- Cited by
- 336
- References
- 15
- FWCI
- 20.71
- Type
- journal article
- Language
- en
- Open access
- gold
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