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The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.

Recent Progress in CRISPR/Cas9 Technology

Mei, Yue, Wang, Yan, Chen, Huiqian, Sun, Zhong Sheng, Ju, Xing-Da·2016·Journal of Genetics and Genomics
DOI 10.1016/j.jgg.2016.01.001
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Abstract

The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system, a simple and efficient tool for genome editing, has experienced rapid progress in its technology and applicability in the past two years. Here, we review the recent advances in CRISPR/Cas9 technology and the ways that have been adopted to expand our capacity for precise genome manipulation. First, we introduce the mechanism of CRISPR/Cas9, including its biochemical and structural implications. Second, we highlight the latest improvements in the CRISPR/Cas9 system, especially Cas9 protein modifications for customization. Third, we review its current applications, in which the versatile CRISPR/Cas9 system was employed to edit the genome, epigenome, or RNA of various organisms. Although CRISPR/Cas9 allows convenient genome editing accompanied by many benefits, we should not ignore the significant ethical and biosafety concerns that it raises. Finally, we discuss the prospective applications and challenges of several promising techniques adapted from CRISPR/Cas9.

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Crossref · 10.1016/j.jgg.2016.01.001

Cites

16 of 16 references verified0 citation errors · 0 not checkable

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  • tracrRNA is a long stretch of loops that serve as a binding scaffold for Cas-9 nuclease.

    “whereas tracrRNA is a long stretch of loops that serve as a binding scaffold for Cas-9 nuclease.”

    From Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing (2021) · cites this paper as [11]

  • HNH domain cleaves the complementary strand, while the RuvC domain cleaves the non-complementary strand of target DNA to produce predominantly blunt-ended DSBs.

    “HNH domain cleaves the complementary strand, while the RuvC domain cleaves the non-complementary strand of target DNA to produce predominantly blunt-ended DSBs.”

    From Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing (2021) · cites this paper as [11]

  • In prokaryotes, guide RNA targets viral DNA; in gene-editing, it can be synthetically designed by combining crRNA and tracrRNA to form sgRNA to target almost any gene sequence.

    “In prokaryotes, the guide RNA is used to target viral DNA, but in the gene-editing tool, it can be synthetically designed by combining crRNA and tracrRNA to form a single guide RNA (sgRNA) in order to target almost any gene sequence supposed to be edited.11”

    From Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing (2021) · cites this paper as [11]

  • Guide RNA is made up of two parts: CRISPR RNA (crRNA) and trans-activating CRISPR RNA (tracrRNA).

    “Guide RNA is made up of two parts, CRISPR RNA (crRNA) and trans-activating CRISPR RNA (tracrRNA).”

    From Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing (2021) · cites this paper as [11]

  • The designed sgRNA recognizes the target sequence in the gene of interest through complementary base pairing.

    “The designed sgRNA recognizes the target sequence in the gene of interest through a complementary base pair.”

    From Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing (2021) · cites this paper as [11]

Citations

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Metadata

Authors
Mei, Y., Wang, Y., Chen, H., Sun, Z. S., & Ju, X.-D.
Venue
Journal of Genetics and Genomics
Publisher
Elsevier BV
Published
2016-02
Volume
43
Issue
2
Pages
63-75
ISSN
1673-8527
DOI
10.1016/j.jgg.2016.01.001
Cited by
148
References
16
Language
en
Licence
Funders
  • Major State Basic Research Development Program of China (2012CB517902, 2012CB517904)
  • Fundamental Research Funds for the Central Universities, (14QNJJ042)
  • National Health and Family Planning Commission of China (201302002)

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