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Rapid blue-light–mediated induction of protein interactions in living cells
Open accessAbstract
Dimerizers allowing inducible control of protein-protein interactions are powerful tools for manipulating biological processes. Here we describe genetically encoded light-inducible protein-interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1 that require no exogenous ligands and dimerize on blue-light exposure with subsecond time resolution and subcellular spatial resolution. We demonstrate the utility of this system by inducing protein translocation, transcription and Cre recombinase–mediated DNA recombination using light.
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Metadata
- Authors
- Kennedy, M. J., Hughes, R. M., Peteya, L. A., Schwartz, J. W., Ehlers, M. D., & Tucker, C. L.
- Venue
- Nature Methods
- Published
- 2010-10-31
- Volume
- 7
- Issue
- 12
- Pages
- 973-975
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2001831734
- Cited by
- 1,120
- References
- 18
- FWCI
- 37.84
- Type
- journal article
- Language
- en
- Open access
- green
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