E-CRISP: fast CRISPR target site identification

Multiplex Genome Engineering Using CRISPR/Cas Systems

Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339

RNA-Guided Human Genome Engineering via Cas9

Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210

Efficient genome editing in zebrafish using a CRISPR-Cas system

Hwang, W. Y., Fu, Y., Reyon, D., Maeder, M. L., Tsai, S. Q., Sander, J. D., Peterson, R. T., Yeh, J.-R. J., & Joung, J. K. (2013) · Nature Biotechnology · doi:10.1038/nbt.2501 · Cited by 2,937

CRISPR RNA Maturation by Trans-Encoded Small RNA and Host Factor RNase III

Deltcheva, E., Chylinski, K., Sharma, C. M., Gonzales, K., Chao, Y., Pirzada, Z. A., Eckert, M. R., Vogel, J., & Charpentier, E. (2011) · Nature · doi:10.1038/nature09886 · Cited by 2,563

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

Jiang, W., Bikard, D., Cox, D., Zhang, F., & Marraffini, L. A. (2013) · Nature Biotechnology · doi:10.1038/nbt.2508 · Cited by 2,429

RNA-programmed genome editing in human cells

Jinek, M., East, A., Cheng, A., Lin, S., Ma, E., & Doudna, J. (2013) · eLife · doi:10.7554/elife.00471 · Cited by 2,098

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

Doench, J. G., Fusi, N., Sullender, M., Hegde, M., Vaimberg, E. W., Donovan, K. F., Smith, I., Tothova, Z., Wilen, C., Orchard, R., et al. (2016) · Nature Biotechnology · doi:10.1038/nbt.3437 · Cited by 4,637

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

Tsai, S. Q., Zheng, Z., Nguyen, N. T., Liebers, M., Topkar, V. V., Thapar, V., Wyvekens, N., Khayter, C., Iafrate, A. J., Le, L. P., et al. (2015) · Nature Biotechnology · doi:10.1038/nbt.3117 · Cited by 2,268

Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR

Haeussler, M., Schönig, K., Eckert, H., Eschstruth, A., Mianné, J., Renaud, J.-B., Schneider-Maunoury, S., Shkumatava, A., Teboul, L., Kent, J., et al. (2016) · Genome biology · doi:10.1186/s13059-016-1012-2 · Cited by 1,756

Off-target Effects in CRISPR/Cas9-mediated Genome Engineering

Zhang, X.-H., Tee, L. Y., Wang, X.-G., Huang, Q.-S., & Yang, S.-H. (2015) · Molecular Therapy - Nucleic Acids · doi:10.1038/mtna.2015.37 · Cited by 1,273

CCTop: An intuitive, flexible and reliable CRISPR/Cas9 target prediction tool

Stemmer, M., Thumberger, T., del Sol Keyer, M., Wittbrodt, J., & Mateo, J. L. (2015) · PLoS ONE · doi:10.1371/journal.pone.0124633 · Cited by 1,088

Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens

Hart, T., Tong, A. H. Y., Chan, K., Van Leeuwen, J., Seetharaman, A., Aregger, M., Chandrashekhar, M., Hustedt, N., Seth, S., Noonan, A., et al. (2017) · G3 Genes|Genomes|Genetics · doi:10.1534/g3.117.041277 · Cited by 599

DeepCRISPR: Optimized CRISPR guide RNA design by deep learning

Chuai, G., Ma, H., Yan, J., Chen, M., Hong, N., Xue, D., Zhou, C., Zhu, C., Chen, K., Duan, B., et al. (2018) · Genome biology · doi:10.1186/s13059-018-1459-4 · Cited by 641

CRISPR/Cas9-based genome editing for disease modeling and therapy: challenges and opportunities for nonviral delivery

Wang, H.-X., Li, M., Lee, C. M., Chakraborty, S., Kim, H.-W., Bao, G., & Leong, K. W. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00799 · Cited by 560

Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9

Li, H. L., Fujimoto, N., Sasakawa, N., Shirai, S., Ohkame, T., Sakuma, T., Tanaka, M., Amano, N., Watanabe, A., Sakurai, H., et al. (2015) · Stem Cell Reports · doi:10.1016/j.stemcr.2014.10.013 · Cited by 471

Am I ready for CRISPR? A user's guide to genetic screens

Doench, J. G. (2018) · Nature Reviews Genetics · doi:10.1038/nrg.2017.97 · Cited by 452

Editing the epigenome: technologies for programmable transcription and epigenetic modulation

Thakore, P. I., Black, J. B., Hilton, I. B., & Gersbach, C. A. (2016) · Nature Methods · doi:10.1038/nmeth.3733 · Cited by 451

Genome-Editing Technologies: Principles and Applications

Gaj, T., Sirk, S. J., Shui, S.-L., & Liu, J. (2016) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a023754 · Cited by 381

The impact of nonsense-mediated mRNA decay on genetic disease, gene editing and cancer immunotherapy

Lindeboom, R. G. H., Vermeulen, M., Lehner, B., & Supek, F. (2019) · Nature Genetics · doi:10.1038/s41588-019-0517-5 · Cited by 327

CRISPR/Cas Systems in Genome Editing: Methodologies and Tools for sgRNA Design, Off‐Target Evaluation, and Strategies to Mitigate Off‐Target Effects

Manghwar, H., Li, B., Ding, X., Hussain, A., Lindsey, K., Zhang, X., & Jin, S. (2020) · Advanced Science · doi:10.1002/advs.201902312 · Cited by 347

Optical Pooled Screens in Human Cells

Feldman, D., Singh, A., Schmid-Burgk, J. L., Carlson, R. J., Mezger, A., Garrity, A. J., Zhang, F., & Blainey, P. C. (2019) · Cell · doi:10.1016/j.cell.2019.09.016 · Cited by 321

Chemoconnectomics: Mapping Chemical Transmission in Drosophila

Deng, B., Li, Q., Liu, X., Cao, Y., Li, B., Qian, Y., Xu, R., Mao, R., Zhou, E., Zhang, W., et al. (2019) · Neuron · doi:10.1016/j.neuron.2019.01.045 · Cited by 340

Prediction of off-target activities for the end-to-end design of CRISPR guide RNAs

Listgarten, J., Weinstein, M., Kleinstiver, B. P., Sousa, A. A., Joung, J. K., Crawford, J., Gao, K., Hoang, L., Elibol, M., Doench, J. G., et al. (2018) · Nature Biomedical Engineering · doi:10.1038/s41551-017-0178-6 · Cited by 336

Stimulus-specific combinatorial functionality of neuronal c-fos enhancers

Joo, J.-Y., Schaukowitch, K., Farbiak, L., Kilaru, G., & Kim, T.-K. (2016) · Nature Neuroscience · doi:10.1038/nn.4170 · Cited by 271

Modified penetrance of coding variants by cis-regulatory variation contributes to disease risk

Castel, S. E., Cervera, A., Mohammadi, P., Aguet, F., Reverter, F., Wolman, A., Guigo, R., Iossifov, I., Vasileva, A., & Lappalainen, T. (2018) · Nature Genetics · doi:10.1038/s41588-018-0192-y · Cited by 217

Cas9-chromatin binding information enables more accurate CRISPR off-target prediction

Singh, R., Kuscu, C., Quinlan, A., Qi, Y., & Adli, M. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv575 · Cited by 217

Pooled In Vitro and In Vivo CRISPR-Cas9 Screening Identifies Tumor Suppressors in Human Colon Organoids

Michels, B. E., Mosa, M. H., Streibl, B. I., Zhan, T., Menche, C., Abou-El-Ardat, K., Darvishi, T., Członka, E., Wagner, S., Winter, J., et al. (2020) · Cell Stem Cell · doi:10.1016/j.stem.2020.04.003 · Cited by 222

Potential pitfalls of CRISPR/Cas9‐mediated genome editing

Peng, R., Lin, G., & Li, J. (2016) · The FEBS Journal · doi:10.1111/febs.13586 · Cited by 213

MEK inhibitors activate Wnt signalling and induce stem cell plasticity in colorectal cancer

Zhan, T., Ambrosi, G., Wandmacher, A. M., Rauscher, B., Betge, J., Rindtorff, N., Häussler, R. S., Hinsenkamp, I., Bamberg, L., Hessling, B., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-09898-0 · Cited by 189

Evaluating and enhancing target specificity of gene-editing nucleases and deaminases

Kim, D., Luk, K., Wolfe, S. A., & Kim, J.-S. (2019) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-013118-111730 · Cited by 171

Insert, remove or replace: A highly advanced genome editing system using CRISPR/Cas9

Ceasar, S. A., Rajan, V., Prykhozhij, S. V., Berman, J. N., & Ignacimuthu, S. (2016) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2016.06.009 · Cited by 151

Rational design of selective allosteric inhibitors of PHGDH and serine synthesis with anti-tumor activity

Wang, Q., Liberti, M. V., Liu, P., Deng, X., Liu, Y., Locasale, J. W., & Lai, L. (2017) · Cell Chemical Biology · doi:10.1016/j.chembiol.2016.11.013 · Cited by 147

RNA Interference (RNAi) Screening in Drosophila

Heigwer, F., Port, F., & Boutros, M. (2018) · Genetics · doi:10.1534/genetics.117.300077 · Cited by 125

CRISPR/Cas9-mediated genome engineering of CHO cell factories: application and perspectives

Lee, J. S., Grav, L. M., Lewis, N. E., & Faustrup Kildegaard, H. (2015) · Biotechnology Journal · doi:10.1002/biot.201500082 · Cited by 117

Genome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions

Hendriks, W. T., Warren, C. R., & Cowan, C. A. (2016) · Cell Stem Cell · doi:10.1016/j.stem.2015.12.002 · Cited by 110

Tools for experimental and computational analyses of off-target editing by programmable nucleases

Bao, X. R., Pan, Y., Lee, C. M., Davis, T. H., & Bao, G. (2021) · Nature Protocols · doi:10.1038/s41596-020-00431-y · Cited by 106

Biased and unbiased methods for the detection of off-target cleavage by CRISPR/Cas9: An overview

Martin, F., Sánchez-Hernández, S., Gutiérrez-Guerrero, A., Pinedo-Gomez, J., & Benabdellah, K. (2016) · International Journal of Molecular Sciences · doi:10.3390/ijms17091507 · Cited by 92

MINSTED nanoscopy enters the Ångström localization range

Weber, M., von der Emde, H., Leutenegger, M., Gunkel, P., Sambandan, S., Khan, T. A., Keller-Findeisen, J., Cordes, V. C., & Hell, S. W. (2023) · Nature Biotechnology · doi:10.1038/s41587-022-01519-4 · Cited by 94

Liver stage malaria infection is controlled by host regulators of lipid peroxidation

Kain, H. S., Glennon, E. K. K., Vijayan, K., Arang, N., Douglass, A. N., Fortin, C. L., Zuck, M., Lewis, A. J., Whiteside, S. L., Dudgeon, D. R., et al. (2020) · Cell Death & Differentiation · doi:10.1038/s41418-019-0338-1 · Cited by 91

Transcriptional regulation with CRISPR-Cas9: principles, advances, and applications

Didovyk, A., Borek, B., Tsimring, L., & Hasty, J. (2016) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2016.06.003 · Cited by 90

Efficient genomic correction methods in human iPS cells using CRISPR–Cas9 system

Li, H. L., Gee, P., Ishida, K., & Hotta, A. (2016) · Methods · doi:10.1016/j.ymeth.2015.10.015 · Cited by 62

Advances in Genome Editing and Application to the Generation of Genetically Modified Rat Models

Chenouard, V., Remy, S., Tesson, L., Ménoret, S., Ouisse, L.-H., Cherifi, Y., & Anegon, I. (2021) · Frontiers in Genetics · doi:10.3389/fgene.2021.615491 · Cited by 57

A Comprehensive, Ethnically Diverse Library of Sickle Cell Disease-Specific Induced Pluripotent Stem Cells

Park, S., Gianotti-Sommer, A., Molina-Estevez, F. J., Vanuytsel, K., Skvir, N., Leung, A., Rozelle, S. S., Shaikho, E. M., Weir, I., Jiang, Z., et al. (2017) · Stem Cell Reports · doi:10.1016/j.stemcr.2016.12.017 · Cited by 50

Revisiting CRISPR/Cas-mediated crop improvement: special focus on nutrition

Kaul, T., Sony, S. K., Verma, R., Motelb, K. F. A., Prakash, A. T., Eswaran, M., Bharti, J., Nehra, M., & Kaul, R. (2020) · Journal of Biosciences · doi:10.1007/s12038-020-00094-7 · Cited by 33

DAZAP2 acts as specifier of the p53 response to DNA damage

Liebl, M. C., Moehlenbrink, J., Becker, H., Raddatz, G., Abdeen, S. K., Aqeilan, R. I., Lyko, F., & Hofmann, T. G. (2021) · Nucleic Acids Research · doi:10.1093/nar/gkab084 · Cited by 20

Untitled work

(2016) · doi:10.13918/j.issn.2095-8137.2016.4.191