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Detecting DNA cytosine methylation using nanopore sequencing
Open accessAbstract
In nanopore sequencing devices, electrolytic current signals are sensitive to base modifications, such as 5-methylcytosine (5-mC). Here we quantified the strength of this effect for the Oxford Nanopore Technologies MinION sequencer. By using synthetically methylated DNA, we were able to train a hidden Markov model to distinguish 5-mC from unmethylated cytosine. We applied our method to sequence the methylome of human DNA, without requiring special steps for library preparation.
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Metadata
- Authors
- Simpson, J. T., Workman, R. E., Zuzarte, P. C., David, M., Dursi, L. J., & Timp, W.
- Venue
- Nature Methods
- Published
- 2017-02-20
- Volume
- 14
- Issue
- 4
- Pages
- 407-410
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2591367881
- Cited by
- 1,136
- References
- 10
- FWCI
- 36.66
- Type
- journal article
- Language
- en
- Open access
- green
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