Cited by 148RNA Research and SplicingRNA regulation and diseaseSingle-cell and spatial transcriptomics

mRNA expression, splicing and editing in the embryonic and adult mouse cerebral cortex

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Dillman, Allissa A, Hauser, David N, Gibbs, J Raphael, Nalls, Michael A, McCoy, Melissa K, Rudenko, Iakov N, Galter, Dagmar, Cookson, Mark R·2013·Nature Neuroscience

Abstract

The complexity of the adult brain is a result of both developmental processes and experience-dependent circuit formation. One way to look at the differences between embryonic and adult brain is to examine gene expression. Previous studies have used microarrays to address this in a global manner. However, the transcriptome is more complex than gene expression levels alone, as alternative splicing and RNA editing generate a diverse set of mature transcripts. Here we report a high-resolution transcriptome data set of mouse cerebral cortex at embryonic and adult stages using RNA sequencing (RNA-Seq). We found many differences in gene expression, splicing and RNA editing between embryonic and adult cerebral cortex. Each data set was validated technically and biologically, and in each case we found our RNA-Seq observations to have predictive validity. We provide this data set and analysis as a resource for understanding gene expression in the embryonic and adult cerebral cortex.

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Authors
Dillman, A. A., Hauser, D. N., Gibbs, J. R., Nalls, M. A., McCoy, M. K., Rudenko, I. N., Galter, D., & Cookson, M. R.
Affiliations
  • National Institutes of Health (US)
  • Karolinska Institutet (SE)
Corresponding author
Cookson, Mark R
Venue
Nature Neuroscience
Published
2013-02-17
Volume
16
Issue
4
Pages
499-506
ISSN
  • 1097-6256 (ISSN-L)
  • 1546-1726
OpenAlex
W2002669455
Cited by
148
References
41
Citations by year
2026: 4 · 2025: 10 · 2024: 7 · 2023: 8 · 2022: 4 · 2021: 10 · 2020: 13 · 2019: 13 · 2018: 14 · 2017: 16 · 2016: 14 · 2015: 14 · 2014: 16 · 2013: 5
FWCI
7.32
Type
journal article
Language
en
Open access
Locations
  • Nature Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • Vetenskapsrådet
  • Swedish Brain Power
  • National Institutes of Health
  • National Institute on Aging (K01AG000947, AG000947, ZIAAG000947)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
Keywords
cerebral cortex, embryonic development, adult brain, gene expression, alternative splicing, RNA editing, splice variants, exon-intron boundaries, transcriptome, mice, brain development
MeSH terms
Age Factors, Animals, Cerebral Cortex, Female, Mice, Inbred C57BL, Pregnancy, RNA Splicing, RNA, Messenger, RNA Editing, Sequence Analysis, RNA, Gene Expression Regulation, Developmental, Mice

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