Cited by 398Biosensors and Analytical DetectionMicrofluidic and Capillary Electrophoresis ApplicationsAdvanced biosensing and bioanalysis techniques

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Advancing the speed, sensitivity and accuracy of biomolecular detection using multi-length-scale engineering

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Kelley, Shana O., Mirkin, Chad A., Walt, David R., Ismagilov, Rustem F., Toner, Mehmet, Sargent, Edward H.·2014·Nature Nanotechnology

DOI 10.1038/nnano.2014.261PMC PMC4472305

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Abstract

Rapid progress in identifying disease biomarkers has increased the importance of creating high-performance detection technologies. Over the last decade, the design of many detection platforms has focused on either the nano or micro length scale. Here, we review recent strategies that combine nano- and microscale materials and devices to produce large improvements in detection sensitivity, speed and accuracy, allowing previously undetectable biomarkers to be identified in clinical samples. Microsensors that incorporate nanoscale features can now rapidly detect disease-related nucleic acids expressed in patient samples. New microdevices that separate large clinical samples into nanocompartments allow precise quantitation of analytes, and microfluidic systems that utilize nanoscale binding events can detect rare cancer cells in the bloodstream more accurately than before. These advances will lead to faster and more reliable clinical diagnostic devices.

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Efficient Isolation and Accurate In Situ Analysis of Circulating Tumor Cells Using Detachable Beads and a High‐Pore‐Density Filter
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Detection of HIV-1 P24 Gag in Plasma by a Nanoparticle-Based Bio-Barcode-Amplification Method
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Direct Genetic Analysis of Ten Cancer Cells: Tuning Sensor Structure and Molecular Probe Design for Efficient mRNA Capture
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Polymerase Chain Reaction-Free, Sample-to-Answer Bacterial Detection in 30 Minutes with Integrated Cell Lysis
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Electrochemical Methods for the Analysis of Clinically Relevant Biomolecules
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Ultrasensitive surface-enhanced Raman scattering detection in common fluids
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Ultrasensitive detection of circulating exosomes with a 3D-nanopatterned microfluidic chip
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See all 398

Authors: Kelley, S. O., Mirkin, C. A., Walt, D. R., Ismagilov, R. F., Toner, M., & Sargent, E. H.
Affiliations: University of Toronto (CA)
Northwestern University (US)
Tufts University (US)
California Institute of Technology (US)
Harvard University (US)
Corresponding author: Kelley, Shana O.
ORCID: Kelley, Shana O.
Mirkin, Chad A.
Walt, David R.
Ismagilov, Rustem F.
Toner, Mehmet
Sargent, Edward H.
Venue: Nature Nanotechnology
Publisher: Springer Science and Business Media LLC
Published: 2014-12
Volume: 9
Issue: 12
Pages: 969-980
ISSN: 1748-3387 (ISSN-L)
1748-3395
DOI: 10.1038/nnano.2014.261
PMC: PMC4472305
OpenAlex: W2082607189
Cited by: 398
References: 102
Citations by year: 2026: 11 · 2025: 22 · 2024: 23 · 2023: 26 · 2022: 43 · 2021: 24 · 2020: 44 · 2019: 38 · 2018: 48 · 2017: 42 · 2016: 40 · 2015: 37
FWCI: 3.39
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.springer.com/tdm (tdm)
Locations: Nature Nanotechnology (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
CaltechAUTHORS (California Institute of Technology) (submittedVersion)
Funders: National Science Foundation
U.S. Department of Defense (W81XWH1110814, W81XWH-11-1-, W81XWH, BC100510)
Prostate Cancer Foundation
Genome Canada
National Institutes of Health (W81XWH)
Defense Advanced Research Projects Agency (HR0011-11-2-0006)
Advanced Research Projects Agency
Canadian Institutes of Health Research
National Institute of Biomedical Imaging and Bioengineering (R01EB012946, P41EB002503)
Topics: Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
Keywords: biomolecule detection, disease biomarkers, nucleic acid detection, microsensors, nanocompartments, microfluidics, clinical diagnostics, detection sensitivity, sample separation
MeSH terms: Humans, Neoplasms, Biomarkers, Tumor, Biosensing Techniques, Nanotechnology

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Advancing the speed, sensitivity and accuracy of biomolecular detection using multi-length-scale engineering

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