A microfabricated nerve-on-a-chip platform for rapid assessment of neural conduction in explanted peripheral nerve fibers
Open accessAbstract
Peripheral nerves are anisotropic and heterogeneous neural tissues. Their complex physiology restricts realistic in vitro models, and high resolution and selective probing of axonal activity. Here, we present a nerve-on-a-chip platform that enables rapid extracellular recording and axonal tracking of action potentials collected from tens of myelinated fibers. The platform consists of microfabricated stimulation and recording microchannel electrode arrays. First, we identify conduction velocities of action potentials traveling through the microchannel and propose a robust data-sorting algorithm using velocity selective recording. We optimize channel geometry and electrode spacing to enhance the algorithm reliability. Second, we demonstrate selective heat-induced neuro-inhibition of peripheral nerve activity upon local illumination of a conjugated polymer (P3HT) blended with a fullerene derivative (PCBM) coated on the floor of the microchannel. We demonstrate the nerve-on-a-chip platform is a versatile tool to optimize the design of implantable peripheral nerve interfaces and test selective neuromodulation techniques ex vivo.
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Microchannel electrodes' good recording properties and ease of use have found use for them in ex vivo applications such as nerve-on-a-chip platforms.
“Their good recording properties and ease of use have, however, found use for them in ex vivo applications such as nerve-on-a-chip platforms (Gribi et al., 2018).”
From Foreign Body Reaction to Implanted Biomaterials and Its Impact in Nerve Neuroprosthetics (2021) · cites this paper as Gribi et al., 2018
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Foreign Body Reaction to Implanted Biomaterials and Its Impact in Nerve Neuroprosthetics
Carnicer-Lombarte, A., Chen, S.-T., Malliaras, G. G., & Barone, D. G. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.622524 · Cited by 454 · see citing works
“Their good recording properties and ease of use have, however, found use for them in ex vivo applications such as nerve-on-a-chip platforms (Gribi et al., 2018).”
BackgroundPhotovoltaic organic interface for neuronal stimulation in the near-infrared
Airaghi Leccardi, M. J. I., Chenais, N. A. L., Ferlauto, L., Kawecki, M., Zollinger, E. G., & Ghezzi, D. (2020) · Communications Materials · doi:10.1038/s43246-020-0023-4 · Cited by 63 · see citing works
Self-citation
- Authors
- Gribi, S., du Bois de Dunilac, S., Ghezzi, D., & Lacour, S. P.
- Affiliations
- École Polytechnique Fédérale de Lausanne (CH)
- Corresponding author
- Lacour, Stéphanie P.
- Venue
- Nature Communications
- Published
- 2018-10-23
- Volume
- 9
- Issue
- 1
- Pages
- 4403-4403
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC6199302
- OpenAlex
- W2896281700
- Cited by
- 72
- References
- 56
- Citations by year
- 2026: 11 · 2025: 3 · 2024: 16 · 2023: 12 · 2022: 8 · 2021: 7 · 2020: 8 · 2019: 7
- FWCI
- 2.51
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Nature Communications (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- Funders
- National Science Foundation
- Fondation Bertarelli
- King's College London
- École Polytechnique Fédérale de Lausanne
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Life Sciences › Neuroscience › Cognitive Neuroscience › Undergraduate Neuroscience Education and Research
- Keywords
- peripheral nerves, nerve conduction, action potential recording, conduction velocity, myelinated axons
- MeSH terms
- Algorithms, Animals, Hot Temperature, Male, Neural Conduction, Neural Inhibition, Peripheral Nerves, Polymers, Rats, Inbred Lew, Microtechnology, Lab-On-A-Chip Devices
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