Reliability of human cortical organoid generation
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The differentiation of pluripotent stem cells in three-dimensional cultures can recapitulate key aspects of brain development, but protocols are prone to variable results. Here we differentiated multiple human pluripotent stem cell lines for over 100 d using our previously developed approach to generate brain-region-specific organoids called cortical spheroids and, using several assays, found that spheroid generation was highly reliable and consistent. We anticipate the use of this approach for large-scale differentiation experiments and disease modeling.
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Guided cortical organoid differentiation yields highly reproducible organoids compared with unguided organoid differentiation.
“Furthermore, several studies have shown that guided cortical organoid differentiation yields highly reproducible organoids as compared to unguided organoid differentiation (72–74).”
From Understanding monocyte-driven neuroinflammation in Alzheimer’s disease using human cortical organoid microphysiological systems (2025) · cites this paper as [73]
A number of groups were able to generate spheroids in chip-based devices, but the spheroids lack spatial complexity and cell types compared to fully-developed organoids.
“A number of groups were able to generate spheroids in chip-based devices171–173, but the spheroids lack spatial complexity and cell types compared to fully-developed organoids.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [173]
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- Authors
- Yoon, S.-J., Elahi, L. S., Pașca, A. M., Marton, R. M., Gordon, A., Revah, O., Miura, Y., Walczak, E. M., Holdgate, G. M., Fan, H. C., Huguenard, J. R., Geschwind, D. H., & Pașca, S. P.
- Affiliations
- Stanford Medicine (US)
- University of California, Los Angeles (US)
- BD Biosciences (United States) (US)
- Corresponding author
- Pașca, Sergiu P.
- ORCID
- Venue
- Nature Methods
- Published
- 2018-12-20
- Volume
- 16
- Issue
- 1
- Pages
- 75-78
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- PMC
- PMC6677388
- OpenAlex
- W2904130550
- Cited by
- 507
- References
- 28
- Citations by year
- 2026: 66 · 2025: 74 · 2024: 57 · 2023: 59 · 2022: 67 · 2021: 70 · 2020: 76 · 2019: 35 · 2012: 1
- FWCI
- 16.09
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nature Methods (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- New York Stem Cell Foundation
- Nature
- National Institutes of Health
- National Institute of Mental Health (R01MH107800, U01MH115745, U01MH115746)
- National Institute of Neurological Disorders and Stroke (R01NS034774)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (K12HD000850)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- cortical organoids, human pluripotent stem cells, brain development, xeno-free culture, feeder-free culture, disease modeling
- MeSH terms
- Cell Line, Humans, Organoids, Reproducibility of Results, Prosencephalon, Sequence Analysis, RNA, Tissue Engineering, Pluripotent Stem Cells, Single-Cell Analysis
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