Cited by 2,238Antioxidant Activity and Oxidative StressRedox biology and oxidative stressFree Radicals and Antioxidants

The DOI matches its Crossref record; 385 of the 385 references it cites were found in a registry or an index.

Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?

Open access

Halliwell, Barry, Whiteman, Matthew·2004·British Journal of Pharmacology

DOI 10.1038/sj.bjp.0705776PMC PMC1574951

Open this paper in CoChat — free account

Abstract

Free radicals and other reactive species (RS) are thought to play an important role in many human diseases. Establishing their precise role requires the ability to measure them and the oxidative damage that they cause. This article first reviews what is meant by the terms free radical, RS, antioxidant, oxidative damage and oxidative stress. It then critically examines methods used to trap RS, including spin trapping and aromatic hydroxylation, with a particular emphasis on those methods applicable to human studies. Methods used to measure oxidative damage to DNA, lipids and proteins and methods used to detect RS in cell culture, especially the various fluorescent "probes" of RS, are also critically reviewed. The emphasis throughout is on the caution that is needed in applying these methods in view of possible errors and artifacts in interpreting the results.

Integrity signals

Registry

Crossref · 10.1038/sj.bjp.0705776

Cites

385 of 385 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay
Benzie, I. F. F., & Strain, J. (1996) · Analytical Biochemistry · doi:10.1006/abio.1996.0292 · Cited by 23,027
Free radicals in biology and medicine
Gutteridge, J. M. C. (1991) · General Pharmacology The Vascular System · doi:10.1016/0306-3623(91)90478-o · Cited by 17,178
Biochemistry and molecular cell biology of diabetic complications
Brownlee, M. (2001) · Nature · doi:10.1038/414813a · Cited by 9,107
Free Radicals in Biology and Medicine
Halliwell, B., & Gutteridge, J. M. C. (2015) · doi:10.1093/acprof:oso/9780198717478.001.0001 · Cited by 8,990
Self-citation
GLUTATHIONE
Meister, A., & Anderson, M. E. (1983) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.52.070183.003431 · Cited by 5,694
Nitric oxide, superoxide, and peroxynitrite: the good, the bad, and ugly
Beckman, J. S., & Koppenol, W. H. (1996) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1996.271.5.c1424 · Cited by 5,513
Oxidative stress: oxidants and antioxidants
Sies, H. (1997) · Experimental Physiology · doi:10.1113/expphysiol.1997.sp004024 · Cited by 4,577
Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress
LeBel, C. P., Ischiropoulos, H., & Bondy, S. C. (1992) · Chemical Research in Toxicology · doi:10.1021/tx00026a012 · Cited by 2,694
The Pecking Order of Free Radicals and Antioxidants: Lipid Peroxidation, α-Tocopherol, and Ascorbate
Buettner, G. R. (1993) · Archives of Biochemistry and Biophysics · doi:10.1006/abbi.1993.1074 · Cited by 2,417 · cited 2×
“…caused by the RS. British Journal of Pharmacology vol 142 (2) Ascorbic acid (vitamin C) reacts with a wide range of free radicals and other RS (Buettner, 1993; Halliwell, 1999c) and one of its oxidation products, semidehydroascorbate radical, can easily be detected by ESR (Buettner &…”
BackgroundMethod
Damage to DNA by reactive oxygen and nitrogen species: role in inflammatory disease and progression to cancer
WISEMAN, H., & HALLIWELL, B. (1996) · Biochemical Journal · doi:10.1042/bj3130017 · Cited by 2,345
Self-citation

Flagged first, then by citations

Show all 385 references

Research works printed but not linked: 2

10.1042/bj3450453 · DOI
10.1172/JCI0215021 · DOI

Sorted by citations

Items 201–300 of 1,000

ROS accumulation and oxidative damage to cell structures in Saccharomyces cerevisiae wine strains during fermentation of high-sugar-containing medium
Landolfo, S., Politi, H., Angelozzi, D., & Mannazzu, I. (2008) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2008.03.008 · Cited by 146
Effect of blueberry ingestion on natural killer cell counts, oxidative stress, and inflammation prior to and after 2.5 h of running
McAnulty, L. S., Nieman, D. C., Dumke, C. L., Shooter, L. A., Henson, D. A., Utter, A. C., Milne, G., & McAnulty, S. R. (2011) · Applied Physiology, Nutrition, and Metabolism · doi:10.1139/h11-120 · Cited by 145
Lycopene: a natural antioxidant for prevention of heat-induced oxidative stress in poultry
Arain, M. A., Mei, Z., Hassan, F., Saeed, M., Alagawany, M. M., Shar, A. H., & Rajput, I. R. (2018) · World s Poultry Science Journal · doi:10.1017/s0043933917001040 · Cited by 145
Protective potential of trans-resveratrol against 4-hydroxynonenal induced damage in PC12 cells
Siddiqui, M. A., Kashyap, M. P., Kumar, V., Al-Khedhairy, A. A., Musarrat, J., & Pant, A. B. (2010) · Toxicology in Vitro · doi:10.1016/j.tiv.2010.06.008 · Cited by 142
Reactive Oxygen Species, Cancer and Anti-Cancer Therapies
Nechifor, M. T., Neagu, T.-M., & Manda, G. (2009) · Current Chemical Biology · doi:10.2174/2212796810903010022 · Cited by 142
Antioxidant properties of casein-phosphopeptides
Kitts, D. D. (2005) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2005.08.009 · Cited by 141
Ameliorative effect of melatonin against gamma-irradiation-induced oxidative stress and tissue injury
El-Missiry, M. A., ., T. A. F., El-Sawi, M. R., & El-Sayed, A. A. (2006) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2006.03.008 · Cited by 144
Production of Reactive Oxygen Species by Complex I (NADH:Ubiquinone Oxidoreductase) from Escherichia coli and Comparison to the Enzyme from Mitochondria
Esterházy, D., King, M. S., Yakovlev, G., & Hirst, J. (2008) · Biochemistry · doi:10.1021/bi702243b · Cited by 144
Antioxidant use in nutraceuticals
Cornelli, U. (2009) · Clinics in Dermatology · doi:10.1016/j.clindermatol.2008.01.010 · Cited by 138
Seizures, antiepileptics, antioxidants and oxidative stress: an insight for researchers
Devi, P. U., Manocha, A., & Vohora, D. (2008) · Expert Opinion on Pharmacotherapy · doi:10.1517/14656560802568230 · Cited by 138
Mitochondria-targeted antioxidants and metabolic modulators as pharmacological interventions to slow ageing
Gruber, J., Fong, S., Chen, C.-B., Yoong, S. L., Pastorin, G., Schaffer, S., Cheah, I. K.-M., & Halliwell, B. B. (2012) · Biotechnology Advances · doi:10.1016/j.biotechadv.2012.09.005 · Cited by 143
Self-citation
Real time monitoring and quantification of reactive oxygen species in breast cancer cell line MCF-7 by 2′,7′–dichlorofluorescin diacetate (DCFDA) assay
Figueroa, D., Asaduzzaman, M., & Young, F. M. (2018) · Journal of Pharmacological and Toxicological Methods · doi:10.1016/j.vascn.2018.03.007 · Cited by 143
The cerebrovascular dysfunction induced by slow pressor doses of angiotensin II precedes the development of hypertension
Capone, C., Faraco, G., Park, L., Cao, X., Davisson, R. L., & Iadecola, C. (2011) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00679.2010 · Cited by 136
Free Radicals Induced Oxidative Stress at a Molecular Level: The Current Status, Challenges and Perspectives of Computational Chemistry Based Protocols
Galano, A. (2017) · Journal of the Mexican Chemical Society · doi:10.29356/jmcs.v59i4.81 · Cited by 136
Real-time in vivo detection of biomaterial-induced reactive oxygen species
Liu, W. F., Ma, M., Bratlie, K. M., Dang, T. T., Langer, R., & Anderson, D. G. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.11.029 · Cited by 134
Lipid peroxidation and generation of hydrogen peroxide in frozen-thawed ram semen cryopreserved in extenders with antioxidants
Maia, M. D. S., Bicudo, S. D., Sicherle, C. C., Rodello, L., & Gallego, I. C. S. (2010) · Animal Reproduction Science · doi:10.1016/j.anireprosci.2010.08.004 · Cited by 134
Non-oncotic properties of albumin. A multidisciplinary vision about the implications for critically ill patients
Ferrer, R., Mateu, X., Maseda, E., Yébenes, J. C., Aldecoa, C., de Haro, C., Ruiz‐Rodríguez, J. C., & Garnacho‐Montero, J. (2017) · Expert Review of Clinical Pharmacology · doi:10.1080/17512433.2018.1412827 · Cited by 136
Effects of glutamine on hydrogen peroxide-induced oxidative damage in intestinal epithelial cells of Jian carp (Cyprinus carpio var. Jian)
Chen, J., Zhou, X., Feng, L., Liu, Y., & Jiang, J. (2008) · Aquaculture · doi:10.1016/j.aquaculture.2008.10.053 · Cited by 131
A ‘tissue model’ to study the plasma delivery of reactive oxygen species
Szili, E. J., Bradley, J. W., & Short, R. D. (2014) · Journal of Physics D Applied Physics · doi:10.1088/0022-3727/47/15/152002 · Cited by 133
The Double-Edged Sword of Oxidative Stress in Skin Damage and Melanoma: From Physiopathology to Therapeutical Approaches
Emanuelli, M., Sartini, D., Molinelli, E., Campagna, R., Pozzi, V., Salvolini, E., Simonetti, O., Campanati, A., & Offidani, A. (2022) · Antioxidants · doi:10.3390/antiox11040612 · Cited by 132
Is oxidative stress a physiological cost of reproduction? An experimental test in house mice
Garratt, M., Vasilaki, A., Stockley, P., McArdle, F., Jackson, M. J., & Hurst, J. L. (2010) · Proceedings of the Royal Society B Biological Sciences · doi:10.1098/rspb.2010.1818 · Cited by 132
Role of oxidative damage in the pathogenesis of viral infections of the nervous system.
Vályi-Nagy, T., & Dermody, T. S. (2005) · PubMed · doi:10.14670/hh-20.957 · Cited by 132
Evidence for the Role of Reactive Nitrogen Species in Polymicrobial Sepsis-Induced Renal Peritubular Capillary Dysfunction and Tubular Injury
Wu, L., Gökden, N., & Mayeux, P. R. (2007) · Journal of the American Society of Nephrology · doi:10.1681/asn.2006121402 · Cited by 133
ROS and Oxidative Stress Are Elevated in Mitosis during Asynchronous Cell Cycle Progression and Are Exacerbated by Mitotic Arrest
Patterson, J. C., Joughin, B. A., van de Kooij, B., Lim, D., Lauffenburger, D. A., & Yaffe, M. B. (2019) · Cell Systems · doi:10.1016/j.cels.2019.01.005 · Cited by 136
Diaphragm Muscle Fiber Dysfunction in Chronic Obstructive Pulmonary Disease
Ottenheijm, C. A. C., Heunks, L. M. A., & Dekhuijzen, P. N. R. (2007) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.200701-020pp · Cited by 130
Adreno-leukodystrophy: Oxidative Stress of Mice and Men
Powers, J. M., Pei, Z., Heinzer, A. K., Brose, R. D., Moser, A. B., Moser, H. W., Watkins, P. A., & Smith, K. D. (2005) · Journal of Neuropathology & Experimental Neurology · doi:10.1097/01.jnen.0000190064.28559.a4 · Cited by 129
Identification and Functional Characterization of a Novel Mitochondrial Carrier for Citrate and Oxoglutarate in Saccharomyces cerevisiae
Castegna, A., Scarcia, P., Agrimi, G., Palmieri, L., Rottensteiner, H., Spera, I., Germinario, L., & Palmieri, F. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.097188 · Cited by 131
Grape Powder Polyphenols Attenuate Atherosclerosis Development in Apolipoprotein E Deficient (E0) Mice and Reduce Macrophage Atherogenicity
Fuhrman, B., Volkova, N., Coleman, R., & Aviram, M. (2005) · Journal of Nutrition · doi:10.1093/jn/135.4.722 · Cited by 131
Involvement of oxidative stress response genes in redox homeostasis, the level of reactive oxygen species, and ageing in
Drakulic, T., Temple, M. D., GUIDO, R., Jarolim, S., Breitenbach, M., Attfield, P. V., & Dawes, I. W. (2005) · FEMS Yeast Research · doi:10.1016/j.femsyr.2005.06.001 · Cited by 130
Pomegranate juice supplementation in chronic obstructive pulmonary disease: a 5-week randomized, double-blind, placebo-controlled trial
Cerdá, B., Soto, C., Albaladejo, M. D., Martínez, P., Sánchez-Gascón, F., Tomás-Barberán, F. A., & Espı́n, J. C. (2005) · European Journal of Clinical Nutrition · doi:10.1038/sj.ejcn.1602309 · Cited by 130
Evaluation of Oxidative Stress in Colorectal Cancer Patients
Chang, D., Wang, F., Zhao, Y., & Pan, H. (2008) · Biomedical and Environmental Sciences · doi:10.1016/s0895-3988(08)60043-4 · Cited by 130
Current Trends in Computational Quantum Chemistry Studies on Antioxidant Radical Scavenging Activity
Spiegel, M. (2022) · Journal of Chemical Information and Modeling · doi:10.1021/acs.jcim.2c00104 · Cited by 135
Oxidative stress responsive transcription factors in cellular signalling transduction mechanisms
Dharshini, L. C. P., Vishnupriya, S., Sakthivel, K. M., & Rasmi, R. R. (2020) · Cellular Signalling · doi:10.1016/j.cellsig.2020.109670 · Cited by 131
Reactive Oxygen and Nitrogen Species in Steatotic Hepatocytes: A Molecular Perspective on the Pathophysiology of Ischemia-Reperfusion Injury in the Fatty Liver
Reiniers, M. J., van Golen, R. F., van Gulik, T. M., & Heger, M. (2013) · Antioxidants and Redox Signaling · doi:10.1089/ars.2013.5486 · Cited by 134
Assessment of biological changes of continuous whole body exposure to static magnetic field and extremely low frequency electromagnetic fields in mice
Hashish, A. H., El-Missiry, M. A., Abdelkader, H. I., & Abou‐Saleh, R. H. (2007) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2007.10.002 · Cited by 128
Antioxidant and Pro-Oxidant Effects of Polyphenolic Compounds and Structure-Activity Relationship Evidence
Guardado, E., Molina, E., Joo, M., & Uriarte, E. (2012) · InTech eBooks · doi:10.5772/29471 · Cited by 126
Experimental evidence for therapeutic potential of taurine in the treatment of nonalcoholic fatty liver disease
Gentile, C. L., Nivala, A. M., Gonzales, J. C., Pfaffenbach, K. T., Wang, D., Wei, Y., Jiang, H. L., Orlicky, D. J., Petersen, D. R., Pagliassotti, M. J., et al. (2011) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00677.2010 · Cited by 126
The Interplay Between Respiratory Supercomplexes and ROS in Aging
Genova, M. L., & Lenaz, G. (2015) · Antioxidants and Redox Signaling · doi:10.1089/ars.2014.6214 · Cited by 129
Cellular redox homeostasis, reactive oxygen species and replicative ageing in Saccharomyces cerevisiae
Ayer, A., Gourlay, C. W., & Dawes, I. W. (2014) · FEMS Yeast Research · doi:10.1111/1567-1364.12114 · Cited by 125
A novel antioxidant formulation designed to treat male infertility associated with oxidative stress: promising preclinical evidence from animal models
Gharagozloo, P., Gutiérrez‐Adán, A., Champroux, A., Noblanc, A., Kocer, A., Calle, A., Pérez‐Cerezales, S., Pericuesta, E., Polhemus, A. M., Moazamian, A., et al. (2016) · Human Reproduction · doi:10.1093/humrep/dev302 · Cited by 129
Differences in cellular damage induced by dielectric barrier discharge plasma between Salmonella Typhimurium and Staphylococcus aureus
Huang, M., Zhuang, H., Zhao, J., Wang, J., Yan, W., & Zhang, J. (2019) · Bioelectrochemistry · doi:10.1016/j.bioelechem.2019.107445 · Cited by 125
Vitamin C
Smirnoff, N. (2011) · Advances in botanical research · doi:10.1016/b978-0-12-385853-5.00003-9 · Cited by 125
The effect of vitamins C and E on biomarkers of oxidative stress depends on baseline level
Block, G., Jensen, C. D., Morrow, J. D., Holland, N., Norkus, E. P., Milne, G. L., Hudes, M. L., Dalvi, T. B., Crawford, P. B., Fung, E. B., et al. (2008) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2008.04.005 · Cited by 124
Use of conventional and -omics based methods for health claims of dietary antioxidants: a critical overview
Knasmüller, S., Nersesyan, A. K., Mišík, M., Gerner, C., Mikulits, W., Ehrlich, V. A., Hoelzl, C., Szakmary, A., & Wagner, K. (2008) · British Journal Of Nutrition · doi:10.1017/s0007114508965752 · Cited by 124
Antioxidants and the Comet assay
Cemeli, E., Baumgartner, A., & Anderson, D. C. (2008) · Mutation Research/Reviews in Mutation Research · doi:10.1016/j.mrrev.2008.05.002 · Cited by 128
The Potential of Lactobacillus spp. for Modulating Oxidative Stress in the Gastrointestinal Tract
Kong, Y., Olejar, K. J., On, S. L. W., & Chelikani, V. (2020) · Antioxidants · doi:10.3390/antiox9070610 · Cited by 127
Vitamins C and E improve regrowth and reduce lipid peroxidation of blackberry shoot tips following cryopreservation
Uchendu, E. E., Leonard, S. W., Traber, M. G., & Reed, B. M. (2009) · Plant Cell Reports · doi:10.1007/s00299-009-0795-y · Cited by 122
Antioxidant Activity/Capacity Measurement. 3. Reactive Oxygen and Nitrogen Species (ROS/RNS) Scavenging Assays, Oxidative Stress Biomarkers, and Chromatographic/Chemometric Assays
Apak, R., Özyürek, M., Güçlü, K., & Çapanoğlu, E. (2015) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.5b04744 · Cited by 126
Antioxidant response mechanism of freshwater microalgae species to reactive oxygen species production: a mini review
Ugya, A. Y., Imam, T. S., Li, A., Ma, J., & Hua, X. (2019) · Chemistry and Ecology · doi:10.1080/02757540.2019.1688308 · Cited by 128
Development of a Selective ESI-MS Derivatization Reagent: Synthesis and Optimization for the Analysis of Aldehydes in Biological Mixtures
Eggink, M., Wijtmans, M., Ekkebus, R., Lingeman, H., de Esch, I. J. P., Kool, J., Niessen, W. M. A., & Irth, H. (2008) · Analytical Chemistry · doi:10.1021/ac801429w · Cited by 122
Integrative neurobiology of energy homeostasis-neurocircuits, signals and mediators
Sánchez-Lasheras, C., Könner, A. C., & Brüning, J. C. (2009) · Frontiers in Neuroendocrinology · doi:10.1016/j.yfrne.2009.08.002 · Cited by 127
Inactivation of Bacteria by Peracetic Acid Combined with Ultraviolet Irradiation: Mechanism and Optimization
Zhang, T., Wang, T., Mejia-Tickner, B., Kissel, J., Xie, X., & Huang, C. (2020) · Environmental Science & Technology · doi:10.1021/acs.est.0c02424 · Cited by 121
Decreased Blood Oxidative Stress after Repeated Muscle-Damaging Exercise
Nikolaidis, M. G., Paschalis, V., Giakas, G., Fatouros, I. G., Koutedakis, Y., Kouretas, D., & Jamurtas, A. Z. (2007) · Medicine & Science in Sports & Exercise · doi:10.1249/mss.0b013e31804ca10c · Cited by 121
Redox regulation of water stress responses in field-grown plants. Role of hydrogen peroxide and ascorbate
Jubany-Marí, T., Munné‐Bosch, S., & Alegre, L. (2010) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2010.01.021 · Cited by 120
The Effect of a Brominated Flame Retardant, Tetrabromobisphenol-A, on Free Radical Formation in Human Neutrophil Granulocytes: The Involvement of the MAP Kinase Pathway and Protein Kinase C
Reistad, T. (2004) · Toxicological Sciences · doi:10.1093/toxsci/kfh298 · Cited by 120
Evaluation of oxidative stress in Insulin Dependent Diabetes Mellitus (IDDM) patients
Ramakrishna, V., & Jailkhani, R. (2007) · Diagnostic Pathology · doi:10.1186/1746-1596-2-22 · Cited by 120
Wine and oxidative stress: Up-to-date evidence of the effects of moderate wine consumption on oxidative damage in humans
Covas, M. I., Gambert, P., Fitó, M., & de la Torre, R. (2009) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2009.06.031 · Cited by 120
Evaluation of oxidative stress and antioxidant profile in patients with oral lichen planus
Ergün, S., Batu, Ş., Warnakulasuriya, S., Özel, S., Önal, A. E., Ofluoğlu, D., Güven, Y., & Tanyeri, H. (2010) · Journal of Oral Pathology and Medicine · doi:10.1111/j.1600-0714.2010.00955.x · Cited by 119
Influence of vitamin C and vitamin E on redox signaling: implications for exercise adaptations
Cobley, J. N., McHardy, H., Morton, J. P., Nikolaidis, M. G., & Close, G. L. (2015) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2015.03.018 · Cited by 118
Betanin as a Multipath Oxidative Stress and Inflammation Modulator: A Beetroot Pigment with Protective Effects on Cardiovascular Disease Pathogenesis
Silva, D. V. T. D., Baião, D. D. S., Ferreira, V. F., & Paschoalin, V. M. F. (2022) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2020.1822277 · Cited by 118
Role of oxidative stress in carbon nanotube-generated health effects
Möller, P., Christophersen, D. V., Jensen, D. M., Kermanizadeh, A., Roursgaard, M., Jacobsen, N. R., Hemmingsen, J. G., Danielsen, P. H., Cao, Y., Jantzen, K., et al. (2014) · Archives of Toxicology · doi:10.1007/s00204-014-1356-x · Cited by 118
In vitro neuroprotective potential of the monoterpenes α-pinene and 1,8-cineole against H 2 O 2 -induced oxidative stress in PC12 cells
Porres-Martínez, M., González‐Burgos, E., Carretero, M. E., & Gómez-Serranillos, M. P. (2016) · Zeitschrift für Naturforschung C · doi:10.1515/znc-2014-4135 · Cited by 118
HULIS Enhancement of Hydroxyl Radical Formation from Fe(II): Kinetics of Fulvic Acid–Fe(II) Complexes in the Presence of Lung Antioxidants
Gonzalez, D. H., Cala, C. K., Peng, Q., & Paulson, S. E. (2017) · Environmental Science & Technology · doi:10.1021/acs.est.7b01299 · Cited by 118
NRF2 Oxidative Stress Induced by Heavy Metals is Cell Type Dependent
Simmons, S. O., Fan, C., Yeoman, K., Wakefield, J., & Ramabhadran, R. (2011) · Current Chemical Genomics · doi:10.2174/1875397301105010001 · Cited by 119
Antioxidant activity and protective effects of peptide lunasin against oxidative stress in intestinal Caco-2 cells
García‐Nebot, M. J., Recio, I., & Hernández‐Ledesma, B. (2013) · Food and Chemical Toxicology · doi:10.1016/j.fct.2013.12.021 · Cited by 117
Bioprospecting for Bioactive Peptide Production by Lactic Acid Bacteria Isolated from Fermented Dairy Food
Tagliazucchi, D., Martini, S., & Solieri, L. (2019) · Fermentation · doi:10.3390/fermentation5040096 · Cited by 117
Anthocyanins: From the Field to the Antioxidants in the Body
Bendokas, V., Stanys, V., Mažeikienė, I., Trumbeckaitė, S., Banienė, R., & Liobikas, J. (2020) · Antioxidants · doi:10.3390/antiox9090819 · Cited by 117
Impact of Mycotoxins on Animals’ Oxidative Status
Mavrommatis, A., Giamouri, E., Tavrizelou, S., Zacharioudaki, M., Danezis, G., Simitzis, P., Zoidis, E., Tsiplakou, E., Pappas, A. C., Georgiou, C. A., et al. (2021) · Antioxidants · doi:10.3390/antiox10020214 · Cited by 117
In ovo Feeding as a Tool for Improving Performance and Gut Health of Poultry: A Review
Das, R., Mishra, P., & Jha, R. (2021) · Frontiers in Veterinary Science · doi:10.3389/fvets.2021.754246 · Cited by 129
A Hydrogen Peroxide-Responsive Hyperpolarized 13C MRI Contrast Agent
Lippert, A. R., Keshari, K. R., Kurhanewicz, J. V., & Chang, C. J. (2011) · Journal of the American Chemical Society · doi:10.1021/ja111589a · Cited by 115
Biochemical phenotypes associated with the mitochondrial ATP6 gene mutations at nt8993
Baracca, A., Sgarbi, G., Mattiazzi, M., Casalena, G., Pagnotta, E., Valentino, M. L., Moggio, M. G., Lenaz, G., Carelli, V., & Solaini, G. (2007) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/j.bbabio.2007.05.005 · Cited by 116
Endothelial cell dysfunction and the vascular complications associated with type 2 diabetes: assessing the health of the endothelium
Ding, H., & Triggle, C. R. (2005) · Vascular Health and Risk Management · doi:10.2147/vhrm.1.1.55.58939 · Cited by 118
Chromatographic and electrophoretic methods for the analysis of biomarkers of oxidative damage to macromolecules (DNA, lipids, and proteins)
Mateos, R., & Bravo, L. (2007) · Journal of Separation Science · doi:10.1002/jssc.200600314 · Cited by 115
Glyphosate Commercial Formulation Causes Cytotoxicity, Oxidative Effects, and Apoptosis on Human Cells
Chaufan, G., Coalova, I., & de Molina, M. D. C. R. (2014) · International Journal of Toxicology · doi:10.1177/1091581813517906 · Cited by 124
Mechanisms and mathematical modeling of ROS production by the mitochondrial electron transport chain
Chenna, S., Koopman, W. J. H., Prehn, J. H. M., & Connolly, N. M. C. (2022) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00455.2021 · Cited by 122
The role of electron-transfer and H-atom donation on the superb antioxidant activity and free radical reaction of curcumin
Barzegar, A. (2012) · Food Chemistry · doi:10.1016/j.foodchem.2012.05.070 · Cited by 114
Requirement of Arsenic Biomethylation for Oxidative DNA Damage
Kojima, C., Ramírez, D. C., Tokar, E. J., Himeno, S., Drobná, Z., Stýblo, M., Mason, R. P., & Waalkes, M. P. (2009) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/djp414 · Cited by 114
Human Vitamin K 2,3-Epoxide Reductase Complex Subunit 1-like 1 (VKORC1L1) Mediates Vitamin K-dependent Intracellular Antioxidant Function
Westhofen, P., Watzka, M., Marinova, M., Hass, M. D. S., Kirfel, G., Müller, J., Bevans, C. G., Müller, C. R., & Oldenburg, J. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.210971 · Cited by 119
A pH-responsive fluorescence probe and photosensitiser based on a tetraamino silicon(iv) phthalocyanine
Jiang, X., Lo, P., Yeung, S., Fong, W., & Ng, D. K. P. (2010) · Chemical Communications · doi:10.1039/c000605j · Cited by 114
Cardiovascular oxidative stress is reduced by an ACE inhibitor in a rat model of streptozotocin-induced diabetes
Fiordaliso, F., Cuccovillo, I., Bianchi, R., Bai, A., Doni, M., Salio, M., Angelis, N. D., Ghezzi, P., Latini, R., & Masson, S. (2006) · Life Sciences · doi:10.1016/j.lfs.2005.12.036 · Cited by 114
Reactive oxygen species function as signaling molecules in controlling plant development and hormonal responses
Martin, R. E., Postiglione, A. E., & Muday, G. K. (2022) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2022.102293 · Cited by 115
Essential oil from Chenopodium ambrosioides and main components: Activity against Leishmania, their mitochondria and other microorganisms
Monzote, L., García, M., Pastor, J., Gil, L., Scull, R., Maes, L. J. R. M., Cos, P., & Gille, L. (2013) · Experimental Parasitology · doi:10.1016/j.exppara.2013.10.007 · Cited by 113
1-Oleyl-lysophosphatidic acid (LPA) promotes polarization of BV-2 and primary murine microglia towards an M1-like phenotype
Plastira, I., Bernhart, E., Goeritzer, M., Reicher, H., Kumble, V. B., Kogelnik, N., Wintersperger, A., Hammer, A., Schlager, S., Jandl, K., et al. (2016) · Journal of Neuroinflammation · doi:10.1186/s12974-016-0701-9 · Cited by 112
ATF4 Regulates CD4+ T Cell Immune Responses through Metabolic Reprogramming
Yang, X., Xia, R., Yue, C., Zhai, W., Du, W., Yang, Q., Cao, H., Chen, X., Obando, D., Zhu, Y., et al. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.04.032 · Cited by 114
Glucose/ROS cascade-responsive ceria nanozymes for diabetic wound healing
Yu, X., Fu, X., Yang, J., Chen, L., Leng, F., Yang, Z., & Yu, C. (2022) · Materials Today Bio · doi:10.1016/j.mtbio.2022.100308 · Cited by 113
N-acetylcysteine attenuates kidney injury in rats subjected to renal ischaemia-reperfusion
Nitescu, N., Ricksten, S., Marcussen, N., Haraldsson, B. S., Nilsson, U., Basu, S., & Guron, G. (2006) · Nephrology Dialysis Transplantation · doi:10.1093/ndt/gfk032 · Cited by 111
Reduced mitochondrial and ascorbate–glutathione activity after artificial ageing in soybean seed
Xin, X., Tian, Q., Yin, G., Chen, X., Zhang, J., Ng, S., & Xin-xiong, L. (2013) · Journal of Plant Physiology · doi:10.1016/j.jplph.2013.09.016 · Cited by 111
Impact of heat stress and nutritional interventions on poultry production
He, S., Arowolo, M. A., Medrano, R. F., Li, S., Yu, Q., Chen, J. Y., & He, J. (2018) · World s Poultry Science Journal · doi:10.1017/s0043933918000727 · Cited by 111
Relationship between inflammation and oxidative stress and its effect on multiple sclerosis
Ramos-González, E. J., Bitzer-Quintero, O. K., Ortíz, G. G., Hernández-Cruz, J. J., & Ramírez-Jirano, L. J. (2021) · Neurología · doi:10.1016/j.nrl.2021.10.003 · Cited by 127
A hypocaloric diet enriched in legumes specifically mitigates lipid peroxidation in obese subjects
Crujeiras, A. B., Parra, D., Abete, I., & Martínez, J. A. (2007) · Free Radical Research · doi:10.1080/10715760601131935 · Cited by 111
Almond Consumption Reduces Oxidative DNA Damage and Lipid Peroxidation in Male Smokers ,
Li, N., Jia, X., Chen, C.-Y. O., Blumberg, J. B., Song, Y., Zhang, W., Zhang, X., Ma, G., & Chen, J. (2007) · Journal of Nutrition · doi:10.1093/jn/137.12.2717 · Cited by 111
The impact of antioxidant agents complimentary to periodontal therapy on oxidative stress and periodontal outcomes: A systematic review
Muniz, F. W. M. G., Nogueira, S. B., Mendes, F. L. V., Rõsing, C. K., Moreira, M. M. S. M., de Andrade, G. M., & Carvalho, R. D. S. (2015) · Archives of Oral Biology · doi:10.1016/j.archoralbio.2015.05.007 · Cited by 110
No cytotoxicity or genotoxicity of graphene and graphene oxide in murine lung epithelial FE1 cells in vitro
Bengtson, S., Kling, K. I., Madsen, A. M., Nørgaard, A. W., Jacobsen, N. R., Clausen, P. A., Alonso, B., Pesquera, A., Zurutuza, A., Ramos, R., et al. (2016) · Environmental and Molecular Mutagenesis · doi:10.1002/em.22017 · Cited by 110
How to measure oxidative stress in an ecological context: methodological and statistical issues
Hõrak, P., & Cohen, A. A. (2010) · Functional Ecology · doi:10.1111/j.1365-2435.2010.01755.x · Cited by 110
Postprandial lipaemia, oxidative stress and endothelial function: a review
Wallace, J. P., Johnson, B. D., Padilla, J., & Mather, K. J. (2010) · International Journal of Clinical Practice · doi:10.1111/j.1742-1241.2009.02146.x · Cited by 109
Metabolomics assessment reveals oxidative stress and altered energy production in the heart after ischemic acute kidney injury in mice
Fox, B. M., Gil, H., Kirkbride-Romeo, L. A., Bagchi, R. A., Wennersten, S. A., Haefner, K. R., Skrypnyk, N. I., Brown, C. N., Soranno, D. E., Gist, K. M., et al. (2019) · Kidney International · doi:10.1016/j.kint.2018.10.020 · Cited by 108
Antioxidant profile of strawberry tree honey and its marker homogentisic acid in several models of oxidative stress
Rosa, A., Tuberoso, C. I. G., Atzeri, A., Melis, M. P., Bifulco, E., & Dessì, M. A. (2011) · Food Chemistry · doi:10.1016/j.foodchem.2011.05.072 · Cited by 107
Hsp70 expression as biomarkers of oxidative stress: Mycotoxins’ exploration
Golli‐Bennour, E. E., & Bacha, H. (2011) · Toxicology · doi:10.1016/j.tox.2011.06.002 · Cited by 107
Anticancer copper pyridine benzimidazole complexes: ROS generation, biomolecule interactions, and cytotoxicity
Prosser, K. E., Chang, S. W., Saraci, F., Le, P. H., & Walsby, C. J. (2016) · Journal of Inorganic Biochemistry · doi:10.1016/j.jinorgbio.2016.11.006 · Cited by 106
Curcumin limits the fibrogenic evolution of experimental steatohepatitis
Vizzutti, F., Provenzano, A., Galastri, S., Milani, S., Delogu, W., Novo, E., Caligiuri, A., Zamara, E., Arena, U., Laffi, G., et al. (2009) · Laboratory Investigation · doi:10.1038/labinvest.2009.112 · Cited by 106

Authors: Halliwell, B., & Whiteman, M.
Affiliations: National University of Singapore (SG)
ORCID: Halliwell, Barry
Whiteman, Matthew
Venue: British Journal of Pharmacology
Publisher: Wiley
Published: 2004-05
Volume: 142
Issue: 2
Pages: 231-255
ISSN: 0007-1188 (ISSN-L)
1476-5381
DOI: 10.1038/sj.bjp.0705776
PMC: PMC1574951
OpenAlex: W1977913875
Cited by: 2,238
References: 385
Citations by year: 2026: 47 · 2025: 51 · 2024: 77 · 2023: 78 · 2022: 92 · 2021: 103 · 2020: 127 · 2019: 106 · 2018: 110 · 2017: 113 · 2016: 123 · 2015: 129 · 2014: 134 · 2013: 141 · 2012: 134
FWCI: 6.98
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor, 1734 d)
Locations: British Journal of Pharmacology (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
National University of Singapore (submittedVersion)
Topics: Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Physical Sciences › Chemistry › Organic Chemistry › Free Radicals and Antioxidants
Keywords: reactive species, free radicals, oxidative stress, oxidative damage, antioxidants, spin trapping, aromatic hydroxylation, fluorescent probes, DNA damage, lipid peroxidation, protein oxidation, in vivo measurement, cell culture, measurement artifacts
MeSH terms: Animals, Electron Spin Resonance Spectroscopy, Humans, Predictive Value of Tests, Spectrometry, Fluorescence, Reactive Oxygen Species, Oxidative Stress, Cell Culture Techniques

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks