The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
Abstract
Engineered endonucleases, such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system, provide a powerful approach for genome editing in animals. However, the microinjection of endonucleases into embryos requires a high skill level, is time consuming and may cause damage to embryos. Here, we demonstrate that the electroporation of endonuclease mRNAs into intact embryos can induce editing at targeted loci and efficiently produce knockout rats. It is noteworthy that the electroporation of ZFNs resulted in an embryonic survival rate (91%) and a genome-editing rate (73%) that were more than 2-fold higher than the corresponding rates from conventional microinjection. Electroporation technology provides a simple and effective method to produce knockout animals.
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“Traditional physical methods such as microinjection [136,137] or electroporation [138,139] have been successfully used with CRISPR to engineer embryonic stem cells and zygotes that later originate genetically modified animals.”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [139]
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“although electroporation is less invasive and allows for the editing of multiple cells at the same time [139]”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [139]
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Citations
Cites
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Cited by
Quadros, R. M., Miura, H., Harms, D. W., Akatsuka, H., Sato, T., Aida, T., Redder, R., Richardson, G. P., Inagaki, Y., Sakai, D., et al. (2017) · Genome Biology · doi:10.1186/s13059-017-1220-4 · Cited by 513
A review of emerging physical transfection methods for CRISPR/Cas9-mediated gene editing
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Loureiro, A., & da Silva, G. J. (2019) · Antibiotics · doi:10.3390/antibiotics8010018 · Cited by 129
Advances in Genome Editing and Application to the Generation of Genetically Modified Rat Models
Chenouard, V., Remy, S., Tesson, L., Ménoret, S., Ouisse, L.-H., Cherifi, Y., & Anegon, I. (2021) · Frontiers in Genetics · doi:10.3389/fgene.2021.615491 · Cited by 57
Pi, W., Feng, G., Liu, M., Nie, C., Chen, C., Wang, J., Wang, L., Wan, P., Liu, C., Zhou, P., et al. (2024) · doi:10.20944/preprints202407.1610.v1 · Cited by 16
Metadata
- Authors
- Kaneko, T., Sakuma, T., Yamamoto, T., & Mashimo, T.
- Venue
- Scientific Reports
- Published
- 2014-10-01
- Volume
- 4
- Issue
- 1
- ISSN
- 2045-2322
- PMC
- PMC4180828
- Cited by
- 204
- References
- 25
- Language
- en
- Licence
- creativecommons.org/licenses/by/4.0 (tdm)
- creativecommons.org/licenses/by/4.0 (vor)
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