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Exosomes derived from alcohol-treated hepatocytes horizontally transfer liver specific miRNA-122 and sensitize monocytes to LPS
Open accessAbstract
Hepatocyte damage and inflammation in monocytes/macrophages are central to the pathogenesis of alcoholic hepatitis (AH). MicroRNAs (miRNAs) regulate all of these processes. MiRNA-122 is abundantly expressed in hepatocytes while monocytes/macrophages have low levels. The role of exosomes in AH and possible cross talk between hepatocyte-derived exosomes and immune cells is not explored yet. Here, we show that the number of exosomes significantly increases in the sera of healthy individuals after alcohol binge drinking and in mice after binge or chronic alcohol consumption. Exosomes isolated from sera after alcohol consumption or from in vitro ethanol-treated hepatocytes contained miRNA-122. Exosomes derived from ethanol-treated Huh7.5 cells were taken up by the recipients THP1 monocytes and horizontally transferred a mature form of liver-specific miRNA-122. In vivo, liver mononuclear cells and Kupffer cells from alcohol-fed mice had increased miRNA-122 levels. In monocytes, miRNA-122 transferred via exosomes inhibited the HO-1 pathway and sensitized to LPS stimulation and increased levels of pro-inflammatory cytokines. Finally, inflammatory effects of exosomes from ethanol-treated hepatocytes were prevented by using RNA interference via exosome-mediated delivery of a miRNA-122 inhibitor. These results demonstrate that first, exosomes mediate communication between hepatocytes and monocytes/macrophages and second, hepatocyte-derived miRNA-122 can reprogram monocytes inducing sensitization to LPS.
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Cites
1 retracted
Li, B., Lee, D., Choi, H.-G., Kim, K.-S., Jeong, G., An, R. B., & Kim, Y. (2012) · Evidence-based Complementary and Alternative Medicine · doi:10.1155/2012/128019 · Cited by 12
RetractedValadi, H., Ekström, K., Bossios, A., Sjöstrand, M., Lee, J. J., & Lötvall, J. O. (2007) · Nature Cell Biology · doi:10.1038/ncb1596 · Cited by 12,834
Extracellular vesicles: biology and emerging therapeutic opportunities
EL Andaloussi, S., Mäger, I., Breakefield, X. O., & Wood, M. J. A. (2013) · Nature Reviews Drug Discovery · doi:10.1038/nrd3978 · Cited by 3,571
B lymphocytes secrete antigen-presenting vesicles.
Raposo, G., Nijman, H. W., Stoorvogel, W., Liejendekker, R., Harding, C. V., Melief, C. J., & Geuze, H. J. (1996) · The Journal of experimental medicine · doi:10.1084/jem.183.3.1161 · Cited by 3,546
Identification of tissue-specific microRNAs from mouse
Lagos-Quintana, M., Rauhut, R., Yalcin, A., Meyer, J., Lendeckel, W., & Tuschl, T. (2002) · Current Biology · doi:10.1016/s0960-9822(02)00809-6 · Cited by 3,355
Rab27a and Rab27b control different steps of the exosome secretion pathway
Ostrowski, M., Carmo, N. B., Krumeich, S., Fanget, I., Raposo, G., Savina, A., Moita, C. F., Schauer, K., Hume, A. N., Freitas, R. P., et al. (2010) · Nature Cell Biology · doi:10.1038/ncb2000 · Cited by 2,750
Nanotubular Highways for Intercellular Organelle Transport
Rustom, A., Saffrich, R., Markovic, I., Walther, P., & Gerdes, H.-H. (2004) · Science · doi:10.1126/science.1093133 · Cited by 2,038
Vesiclepedia: A Compendium for Extracellular Vesicles with Continuous Community Annotation
Kalra, H., Simpson, R. J., Ji, H., Aikawa, E., Altevogt, P., Askenase, P., Bond, V. C., Borràs, F. E., Breakefield, X., Budnik, V., et al. (2012) · PLoS Biology · doi:10.1371/journal.pbio.1001450 · Cited by 1,367
Reduced stress defense in heme oxygenase 1-deficient cells
Poss, K. D., & Tonegawa, S. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.20.10925 · Cited by 1,291
Mechanisms of Cell Protection by Heme Oxygenase-1
Gozzelino, R., Jeney, V., & Soares, M. P. (2010) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pharmtox.010909.105600 · Cited by 1,241
Decoding cell death signals in liver inflammation
Brenner, C., Galluzzi, L., Kepp, O., & Kroemer, G. (2013) · Journal of Hepatology · doi:10.1016/j.jhep.2013.03.033 · Cited by 1,022
Grange, C., Tapparo, M., Collino, F., Vitillo, L., Damasco, C., Deregibus, M. C., Tetta, C., Bussolati, B., & Camussi, G. (2011) · Cancer Research · doi:10.1158/0008-5472.can-11-0241 · Cited by 851
Gatti, S., Bruno, S., Deregibus, M. C., Sordi, A., Cantaluppi, V., Tetta, C., & Camussi, G. (2011) · Nephrology Dialysis Transplantation · doi:10.1093/ndt/gfr015 · Cited by 813
Ono, M., Kosaka, N., Tominaga, N., Yoshioka, Y., Takeshita, F., Takahashi, R.-U., Yoshida, M., Tsuda, H., Tamura, K., & Ochiya, T. (2014) · Science Signaling · doi:10.1126/scisignal.2005231 · Cited by 696
Codon-Optimized Gaussia Luciferase cDNA for Mammalian Gene Expression in Culture and in Vivo
Tannous, B. A., Kim, D.-E., Fernandez, J. L., Weissleder, R., & Breakefield, X. O. (2005) · Molecular Therapy · doi:10.1016/j.ymthe.2004.10.016 · Cited by 682
Bala, S., Petrasek, J., Mundkur, S., Catalano, D., Levin, I., Ward, J., Alao, H., Kodys, K., & Szabo, G. (2012) · Hepatology · doi:10.1002/hep.25873 · Cited by 631
Self-citationCurrent methods for the isolation of extracellular vesicles
Momen-Heravi, F., Balaj, L., Alian, S., Mantel, P.-Y., Halleck, A. E., Trachtenberg, A. J., Soria, C. E., Oquin, S., Bonebreak, C. M., Saracoglu, E., et al. (2013) · bchm · doi:10.1515/hsz-2013-0141 · Cited by 578
Self-citationSilverman, J. M., Clos, J., de'Oliveira, C. C., Shirvani, O., Fang, Y., Wang, C., Foster, L. J., & Reiner, N. E. (2010) · Journal of Cell Science · doi:10.1242/jcs.056465 · Cited by 464
Petrasek, J., Iracheta-Vellve, A., Csak, T., Satishchandran, A., Kodys, K., Kurt-Jones, E. A., Fitzgerald, K. A., & Szabo, G. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1308331110 · Cited by 452
Self-citationHritz, I., Mandrekar, P., Velayudham, A., Catalano, D., Dolganiuc, A., Kodys, K., Kurt‐Jones, E., & Szabo, G. (2008) · Hepatology · doi:10.1002/hep.22470 · Cited by 415
Self-citationWang, X., Huang, W., Liu, G., Cai, W., Millard, R. W., Wang, Y., Chang, J., Peng, T., & Fan, G.-C. (2014) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2014.05.001 · Cited by 405
Bala, S., Marcos, M., Kodys, K., Csak, T., Catalano, D., Mandrekar, P., & Szabo, G. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.145870 · Cited by 361
Self-citationBhatnagar, S., & Schorey, J. S. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m702277200 · Cited by 333
Acute Binge Drinking Increases Serum Endotoxin and Bacterial DNA Levels in Healthy Individuals
Bala, S., Marcos, M., Gattu, A., Catalano, D., & Szabo, G. (2014) · PLoS ONE · doi:10.1371/journal.pone.0096864 · Cited by 311
Self-citationFang, J.-H., Zhou, H.-C., Zeng, C., Yang, J., Liu, Y., Huang, X., Zhang, J.-P., Guan, X.-Y., & Zhuang, S.-M. (2011) · Hepatology · doi:10.1002/hep.24577 · Cited by 307
Exosome-mediated delivery of functionally active miRNA-155 inhibitor to macrophages
Momen-Heravi, F., Bala, S., Bukong, T., & Szabo, G. (2014) · Nanomedicine: Nanotechnology, Biology and Medicine · doi:10.1016/j.nano.2014.03.014 · Cited by 306
Self-citationInflammation in Alcoholic Liver Disease
Wang, H. J., Gao, B., Zakhari, S., & Nagy, L. E. (2012) · Annual Review of Nutrition · doi:10.1146/annurev-nutr-072610-145138 · Cited by 297
Exosomes and Cancer: A Newly Described Pathway of Immune Suppression
Zhang, H.-G., & Grizzle, W. E. (2011) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-10-1489 · Cited by 285
Lima, L. G., Chammas, R., Monteiro, R. Q., Moreira, M. E. C., & Barcinski, M. A. (2009) · Cancer Letters · doi:10.1016/j.canlet.2009.03.041 · Cited by 252
Nanoparticle analysis of circulating cell-derived vesicles in ovarian cancer patients
Gercel-Taylor, C., Atay, S., Tullis, R. H., Kesimer, M., & Taylor, D. D. (2012) · Analytical Biochemistry · doi:10.1016/j.ab.2012.06.004 · Cited by 232
Lippai, D., Bala, S., Petrasek, J., Csak, T., Levin, I., Kurt-Jones, E. A., & Szabo, G. (2013) · Journal of Leukocyte Biology · doi:10.1189/jlb.1212659 · Cited by 215
Self-citationInduction of Heme Oxygenase-1 In Vivo Suppresses NADPH Oxidase–Derived Oxidative Stress
Datla, S. R., Dusting, G. J., Mori, T. A., Taylor, C. J., Croft, K. D., & Jiang, F. (2007) · Hypertension · doi:10.1161/hypertensionaha.107.092296 · Cited by 197
Dubyak, G. R. (2012) · Cellular Microbiology · doi:10.1111/cmi.12001 · Cited by 189
Taillé, C., El-Benna, J., Lanone, S., Dang, M.-C., Ogier-Denis, E., Aubier, M., & Boczkowski, J. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m310661200 · Cited by 181
Anderson, H. C., Mulhall, D., & Garimella, R. (2010) · Laboratory Investigation · doi:10.1038/labinvest.2010.152 · Cited by 176
The emerging roles of microvesicles in liver diseases
Lemoinne, S., Thabut, D., Housset, C., Moreau, R., Valla, D., Boulanger, C. M., & Rautou, P.-E. (2014) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/nrgastro.2014.7 · Cited by 172
Shen, Z., Ajmo, J. M., Rogers, C. Q., Liang, X., Le, L., Murr, M. M., Peng, Y., & You, M. (2009) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00016.2009 · Cited by 172
Microvesicles/exosomes as potential novel biomarkers of metabolic diseases
Müller, G. (2012) · Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy · doi:10.2147/dmso.s32923 · Cited by 170
Alternative Methods for Characterization of Extracellular Vesicles
Momen‐Heravi, F., Balaj, L., Alian, S., Tigges, J., Toxavidis, V., Ericsson, M., Distel, R. J., Ivanov, A. R., Skog, J., & Kuo, W. P. (2012) · Frontiers in Physiology · doi:10.3389/fphys.2012.00354 · Cited by 168
Self-citationShan, Y., Zheng, J., Lambrecht, R. W., & Bonkovsky, H. L. (2007) · Gastroenterology · doi:10.1053/j.gastro.2007.08.002 · Cited by 165
Soucy-Faulkner, A., Mukawera, E., Fink, K., Martel, A., Jouan, L., Nzengue, Y., Lamarre, D., Velde, C. V., & Grandvaux, N. (2010) · PLoS Pathogens · doi:10.1371/journal.ppat.1000930 · Cited by 146
Emerging technologies in extracellular vesicle-based molecular diagnostics
Jia, S., Zocco, D., Samuels, M. L., Chou, M. F., Chammas, R. J., Skog, J., Zarovni, N., Momen‐Heravi, F., & Kuo, W. P. (2014) · Expert Review of Molecular Diagnostics · doi:10.1586/14737159.2014.893828 · Cited by 127
Self-citationExosomes Released by Islet-Derived Mesenchymal Stem Cells Trigger Autoimmune Responses in NOD Mice
Rahman, M. J., Regn, D., Bashratyan, R., & Dai, Y. D. (2013) · Diabetes · doi:10.2337/db13-0859 · Cited by 124
Drechsler, Y., Dolganiuc, A., Norkina, O., Romics, L., Li, W., Kodys, K., Bach, F. H., Mandrekar, P., & Szabó, G. (2006) · The Journal of Immunology · doi:10.4049/jimmunol.177.4.2592 · Cited by 121
Self-citationManfè, V., Biskup, E., Rosbjerg, A., Kamstrup, M., Skov, A., Lerche, C., Lauenborg, B., Ødum, N., & Gniadecki, R. (2012) · PLoS ONE · doi:10.1371/journal.pone.0029541 · Cited by 116
Cell vesiculation and immunopathology: implications in cerebral malaria
Coltel, N., Combes, V., Wassmer, S. C., Chimini, G., & Grau, G. E. (2006) · Microbes and Infection · doi:10.1016/j.micinf.2006.04.006 · Cited by 75
MiR-122 modulates type I interferon expression through blocking suppressor of cytokine signaling 1
Li, A., Song, W., Qian, J., Li, Y., He, J., Zhang, Q., Li, W., Zhai, A., Kao, W., Hu, Y., et al. (2013) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2013.01.008 · Cited by 67
Norkina, O., Dolganiuc, A., Catalano, D., Kodys, K., Mandrekar, P., Syed, A., Efros, M., & Szabó, G. (2008) · Alcoholism Clinical and Experimental Research · doi:10.1111/j.1530-0277.2008.00726.x · Cited by 64
Self-citationNZ-GMP Approved Serum Improve hDPSC Osteogenic Commitment and Increase Angiogenic Factor Expression
Spina, A., Montella, R., Liccardo, D., ROSA, A. D., Laino, L., Mitsiadis, T. A., & Noce, M. L. (2016) · Frontiers in Physiology · doi:10.3389/fphys.2016.00354 · Cited by 21
Cited by
Kim, K. M., Abdelmohsen, K., Mustapic, M., Kapogiannis, D., & Gorospe, M. (2017) · WIREs RNA · doi:10.1002/wrna.1413 · Cited by 458
Extracellular vesicles in liver disease and potential as biomarkers and therapeutic targets
Szabo, G., & Momen-Heravi, F. (2017) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/nrgastro.2017.71 · Cited by 285
Self-citationExtracellular Vesicles in Liver Pathobiology: Small Particles With Big Impact
Hirsova, P., Ibrahim, S. H., Verma, V. K., Morton, L. A., Shah, V. H., LaRusso, N. F., Gores, G. J., & Malhi, H. (2016) · Hepatology · doi:10.1002/hep.28814 · Cited by 229
Lee, Y.-S., Kim, S. Y., Ko, E., Lee, J.-H., Yi, H.-S., Yoo, Y. J., Je, J., Suh, S. J., Jung, Y. K., Kim, J. H., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-03389-2 · Cited by 233
Church, R. J., Kullak‐Ublick, G. A., Aubrecht, J., Bonkovsky, H. L., Chalasani, N., Fontana, R. J., Goepfert, J. C., Hackman, F., King, N. M. P., Kirby, S., et al. (2019) · Hepatology · doi:10.1002/hep.29802 · Cited by 231
Metadata
- Authors
- Momen-Heravi, F., Bala, S., Kodys, K., & Szabo, G.
- Venue
- Scientific Reports
- Published
- 2015-05-14
- Volume
- 5
- Issue
- 1
- Pages
- 9991-9991
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W259769697
- Cited by
- 347
- References
- 49
- FWCI
- 11.84
- Type
- journal article
- Language
- en
- Open access
- gold
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