The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Dynamic single cell culture array
Abstract
It is important to quantify the distribution of behavior amongst a population of individual cells to reach a more complete quantitative understanding of cellular processes. Improved high-throughput analysis of single cell behavior requires uniform conditions for individual cells with controllable cell-cell interactions, including diffusible and contact elements. Uniform cell arrays for static culture of adherent cells have previously been constructed using protein micropatterning techniques but lack the ability to control diffusible secretions. Here we present a microfluidic-based dynamic single cell culture array that allows both arrayed culture of individual adherent cells and dynamic control of fluid perfusion with uniform environments for individual cells. In our device no surface modification is required and cell loading is done in less than 30 seconds. The device consists of arrays of physical U-shaped hydrodynamic trapping structures with geometries that are biased to trap only single cells. HeLa cells were shown to adhere at a similar rate in the trapping array as on a control glass substrate. Additionally, rates of cell death and division were comparable to the control experiment. Approximately 100 individual isolated cells were observed growing and adhering in a field of view spanning approximately 1 mm(2) with greater than 85% of cells maintained within the primary trapping site after 24 hours. Also, greater than 90% of cells were adherent and only 5% had undergone apoptosis after 24 hours of perfusion culture within the trapping array. We anticipate uses in single cell analysis of drug toxicity with physiologically relevant perfused dosages as well as investigation of cell signaling pathways and systems biology.
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Citations
Cites
Geometric Control of Cell Life and Death
Chen, C. S., Mrksich, M., Huang, S., Whitesides, G. M., & Ingber, D. E. (1997) · Science · doi:10.1126/science.276.5317.1425 · Cited by 4,657
Capturing complex 3D tissue physiology in vitro
Griffith, L. G., & Swartz, M. A. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1858 · Cited by 2,290
Nanotubular Highways for Intercellular Organelle Transport
Rustom, A., Saffrich, R., Markovic, I., Walther, P., & Gerdes, H.-H. (2004) · Science · doi:10.1126/science.1093133 · Cited by 2,038
Microscale technologies for tissue engineering and biology
Khademhosseini, A., Langer, R., Borenstein, J., & Vacanti, J. P. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0507681102 · Cited by 1,558
A microfluidic culture platform for CNS axonal injury, regeneration and transport
Taylor, A. M., Blurton-Jones, M., Rhee, S. W., Cribbs, D. H., Cotman, C. W., & Jeon, N. L. (2005) · Nature Methods · doi:10.1038/nmeth777 · Cited by 1,153
Control, exploitation and tolerance of intracellular noise
Rao, C. V., Wolf, D. M., & Arkin, A. P. (2002) · Nature · doi:10.1038/nature01258 · Cited by 1,006
The extracellular matrix guides the orientation of the cell division axis
Théry, M., Racine, V., Pépin, A., Piel, M., Chen, Y., Sibarita, J.-B., & Bornens, M. (2005) · Nature Cell Biology · doi:10.1038/ncb1307 · Cited by 780
Microengineering of Cellular Interactions
Folch, A., & Toner, M. (2000) · Annual Review of Biomedical Engineering · doi:10.1146/annurev.bioeng.2.1.227 · Cited by 602
Continuous perfusion microfluidic cell culture array for high‐throughput cell‐based assays
Hung, P. J., Lee, P. J., Sabounchi, P., Lin, R., & Lee, L. P. (2005) · Biotechnology and Bioengineering · doi:10.1002/bit.20289 · Cited by 522
Self-citationLarge-Scale Single-Cell Trapping And Imaging Using Microwell Arrays
Rettig, J. R., & Folch, A. (2005) · Analytical Chemistry · doi:10.1021/ac0505977 · Cited by 502
Engineering gene expression and protein synthesis by modulation of nuclear shape
Thomas, C. H., Collier, J. H., Sfeir, C. S., & Healy, K. E. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.032668799 · Cited by 482
Di Carlo, D., Aghdam, N., & Lee, L. P. (2006) · Analytical Chemistry · doi:10.1021/ac060541s · Cited by 458
Self-citationComputerized microfluidic cell culture using elastomeric channels and Braille displays
Gu, W., Zhu, X., Futai, N., Cho, B. S., & Takayama, S. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0404353101 · Cited by 403
Microenvironment design considerations for cellular scale studies
Walker, G. M., Zeringue, H. C., & Beebe, D. J. (2004) · Lab on a Chip · doi:10.1039/b311214d · Cited by 388
Differentiation-on-a-chip: A microfluidic platform for long-term cell culture studies
Tourovskaia, A., Figueroa-Masot, X., & Folch, A. (2005) · Lab on a Chip · doi:10.1039/b405719h · Cited by 363
CONNEXINS AND CELL SIGNALING IN DEVELOPMENT AND DISEASE
Wei, C.-J., Xu, X., & Lo, C. W. (2004) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.19.111301.144309 · Cited by 360
Patterning Mammalian Cells Using Elastomeric Membranes
Ostuni, E., Kane, R., Chen, C. S., Ingber, D. E., & Whitesides, G. M. (2000) · Langmuir · doi:10.1021/la000382m · Cited by 316
Patterned cell culture inside microfluidic devices
Rhee, S. W., Taylor, A. M., Tu, C. H., Cribbs, D. H., Cotman, C. W., & Jeon, N. L. (2005) · Lab on a Chip · doi:10.1039/b403091e · Cited by 283
Microfluidic arrays for logarithmically perfused embryonic stem cell culture
Kim, L., Vahey, M. D., Lee, H.-Y., & Voldman, J. (2006) · Lab on a Chip · doi:10.1039/b511718f · Cited by 262
Mechanotransduction in endothelial cell migration
Li, S., Huang, N. F., & Hsu, S. (2005) · Journal of Cellular Biochemistry · doi:10.1002/jcb.20614 · Cited by 260
Hung, P. J., Lee, P. J., Sabounchi, P., Aghdam, N., Lin, R., & Lee, L. P. (2005) · Lab on a Chip · doi:10.1039/b410743h · Cited by 259
Self-citationComputer-Controlled Microcirculatory Support System for Endothelial Cell Culture and Shearing
Song, J. W., Gu, W., Futai, N., Warner, K. A., Nor, J. E., & Takayama, S. (2005) · Analytical Chemistry · doi:10.1021/ac050131o · Cited by 249
Jiang, X., Ferrigno, R., Mrksich, M., & Whitesides, G. M. (2003) · Journal of the American Chemical Society · doi:10.1021/ja029485c · Cited by 235
Simple Approach to Micropattern Cells on Common Culture Substrates by Tuning Substrate Wettability
Tan, J. L., Liu, W., Nelson, C. M., Raghavan, S., & Chen, C. S. (2004) · Tissue Engineering · doi:10.1089/1076327041348365 · Cited by 234
Selective Deposition of Proteins and Cells in Arrays of Microwells
Ostuni, E., Chen, C. S., Ingber, D. E., & Whitesides, G. M. (2001) · Langmuir · doi:10.1021/la001372o · Cited by 232
Eph signaling: a structural view
Himanen, J.-P., & Nikolov, D. B. (2003) · Trends in Neurosciences · doi:10.1016/s0166-2236(02)00005-x · Cited by 202
Microfabricated platform for studying stem cell fates
Chin, V. I., Taupin, P., Sanga, S., Scheel, J., Gage, F. H., & Bhatia, S. N. (2004) · Biotechnology and Bioengineering · doi:10.1002/bit.20254 · Cited by 202
A Scalable Addressable Positive-Dielectrophoretic Cell-Sorting Array
Taff, B. M., & Voldman, J. (2005) · Analytical Chemistry · doi:10.1021/ac0513616 · Cited by 191
Controlling Mammalian Cell Interactions on Patterned Polyelectrolyte Multilayer Surfaces
Berg, M. C., Yang, S. Y., Hammond, P. T., & Rubner, M. F. (2004) · Langmuir · doi:10.1021/la0355489 · Cited by 173
Lidstrom, M. E., & Meldrum, D. R. (2003) · Nature Reviews Microbiology · doi:10.1038/nrmicro755 · Cited by 162
Tourovskaia, A., Barber, T., Wickes, B. T., Hirdes, D., Grin, B., Castner, D. G., Healy, K. E., & Folch, A. (2003) · Langmuir · doi:10.1021/la0267948 · Cited by 159
Assembly of multicellular constructs and microarrays of cells using magnetic nanowires
Monica TanaseCurrent address: Department o, Felton, E. J., Gray, D. S., Hultgren, A., Chen, C. S., & Reich, D. H. (2005) · Lab on a Chip · doi:10.1039/b500243e · Cited by 143
Direct Patterning of Protein‐ and Cell‐Resistant Polymeric Monolayers and Microstructures
Khademhosseini, A., Jon, S., Suh, K. Y., Tran, T. ‐. T., Eng, G. M., Yeh, J., Seong, J., & Langer, R. (2003) · Advanced Materials · doi:10.1002/adma.200305433 · Cited by 131
Revzin, A., Rajagopalan, P., Tilles, A. W., Francois Berthiaume, B., Yarmush, M. L., & Toner, M. (2004) · Langmuir · doi:10.1021/la035827w · Cited by 104
NanoLiterBioReactor: Long-Term Mammalian Cell Culture at Nanofabricated Scale
Prokop, A., Prokop, Z., Schaffer, D. K., Kozlov, E., Wikswo, J. P., Cliffel, D. E., & Baudenbacher, F. J. (2004) · Biomedical Microdevices · doi:10.1023/b:bmmd.0000048564.37800.d6 · Cited by 97
Handheld recirculation system and customized media for microfluidic cell culture
Futai, N., Gu, W., Song, J. W., & Takayama, S. (2005) · Lab on a Chip · doi:10.1039/b510901a · Cited by 97
Petronis, Š., Stangegaard, M., Christensen, C. B. V., et al. (2006) · BioTechniques · doi:10.2144/000112122 · Cited by 78
Cited by
Microfluidics: reframing biological enquiry
Duncombe, T. A., Tentori, A. M., & Herr, A. E. (2015) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm4041 · Cited by 345
Microfluidic self-assembly of tumor spheroids for anticancer drug discovery
Wu, L. Y., Di Carlo, D., & Lee, L. P. (2008) · Biomedical Microdevices · doi:10.1007/s10544-007-9125-8 · Cited by 268
Self-citationOverview of single-cell analyses: microdevices and applications
Lindström, S., & Andersson-Svahn, H. (2010) · Lab on a Chip · doi:10.1039/c0lc00150c · Cited by 260
Microfluidics within a well: an injection-molded plastic array 3D culture platform
Lee, Y., Choi, J. W., Yu, J., Park, D., Ha, J., Son, K., Lee, S., Chung, M., Kim, H.-Y., & Jeon, N. L. (2018) · Lab on a Chip · doi:10.1039/c8lc00336j · Cited by 99
High-throughput analysis of signals regulating stem cell fate and function
Underhill, G. H., & Bhatia, S. N. (2007) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2007.05.036 · Cited by 93
Metadata
- Authors
- Carlo, D. D., Wu, L. Y., & Lee, L. P.
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2006
- Volume
- 6
- Issue
- 11
- Pages
- 1445
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W2071624306
- Cited by
- 707
- References
- 37
- FWCI
- 21.21
- Type
- journal article
- Language
- en
- Open access
- closed
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