Cited by 113Stress Responses and CortisolAnalytical Methods in PharmaceuticalsMetabolomics and Mass Spectrometry Studies

Quantification of cortisol in human eccrine sweat by liquid chromatography – tandem mass spectrometry

Open access
Jia, Min, Chew, Wade M., Feinstein, Yelena, Skeath, Perry, Sternberg, Esther M.·2016·The Analyst

Abstract

Cortisol was separated from its isomers and quantified by HPLC-MS/MS in human eccrine sweat samples.

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Registry

Crossref · 10.1039/c5an02387d

Cites

32 of 32 references verified0 citation errors · 0 not checkable

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  • Cortisol is responsible for maintaining homeostasis in the body via the regulation of neural, immune, cardiovascular, metabolic, and endocrine systems.

    “Cortisol is responsible for maintaining homeostasis in the body via the regulation of neural, immune, cardiovascular, metabolic, and endocrine systems.102”
  • Cortisol is a steroid hormone secreted from the adrenal glands in response to stress as a product in the hypothalamic-pituitary adrenal pathway.

    “Cortisol is a steroid hormone secreted from the adrenal glands in response to stress as a product in the hypothalamic–pituitary adrenal pathway.102”
  • Jia et al.'s study detected one isomer in eccrine sweat that had a similar hydrophobicity, retention time, and fragmentation patterns to that of cortisol.

    “The study detected one isomer that had a similar hydrophobicity, retention time, and fragmentation patterns to that of cortisol found in eccrine sweat.”
  • Prior work to measure stress-related biomarkers has utilized high-performance liquid chromatography (HPLC), enzyme-linked immunosorbent assay, radioimmunoassay kit, or a HPLC mass spectrometry (MS) system.

    “Prior work to measure stress-related biomarkers has utilized high-performance liquid chromatography (HPLC),99 enzyme-linked immunosorbent assay,100 radioimmunoassay kit,101 or a HPLC mass spectrometry (MS) system.102”

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Authors
Jia, M., Chew, W. M., Feinstein, Y., Skeath, P., & Sternberg, E. M.
Affiliations
University of Arizona (US)
Corresponding author
  • Jia, Min
  • Sternberg, Esther M.
Venue
The Analyst
Published
2016
Volume
141
Issue
6
Pages
2053-2060
ISSN
  • 0003-2654 (ISSN-L)
  • 1364-5528
OpenAlex
W2260554414
Cited by
113
References
32
Citations by year
2026: 16 · 2025: 6 · 2024: 22 · 2023: 11 · 2022: 14 · 2021: 15 · 2020: 14 · 2019: 11 · 2018: 3 · 2017: 1
FWCI
3.19
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • The Analyst (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
Air Force Research Laboratory (FA8650-13-2-7311)
Topics
  • Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
  • Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
Keywords
cortisol, eccrine sweat, sweat biomarkers, stress biomarkers, non-invasive stress assessment, steroid quantification, atmospheric pressure chemical ionization, multiple reaction monitoring
MeSH terms
Chromatography, High Pressure Liquid, Exocrine Glands, Humans, Hydrocortisone, Sweat, Time Factors, Reproducibility of Results, Clinical Chemistry Tests, Analytic Sample Preparation Methods, Tandem Mass Spectrometry

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