Quantification of cortisol in human eccrine sweat by liquid chromatography – tandem mass spectrometry
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Cortisol was separated from its isomers and quantified by HPLC-MS/MS in human eccrine sweat samples.
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Cortisol is responsible for maintaining homeostasis in the body via the regulation of neural, immune, cardiovascular, metabolic, and endocrine systems.
“Cortisol is responsible for maintaining homeostasis in the body via the regulation of neural, immune, cardiovascular, metabolic, and endocrine systems.102”
From Wearable sensors for monitoring the physiological and biochemical profile of the athlete (2019) · cites this paper as [102]
Cortisol is a steroid hormone secreted from the adrenal glands in response to stress as a product in the hypothalamic-pituitary adrenal pathway.
“Cortisol is a steroid hormone secreted from the adrenal glands in response to stress as a product in the hypothalamic–pituitary adrenal pathway.102”
From Wearable sensors for monitoring the physiological and biochemical profile of the athlete (2019) · cites this paper as [102]
Jia et al.'s study detected one isomer in eccrine sweat that had a similar hydrophobicity, retention time, and fragmentation patterns to that of cortisol.
“The study detected one isomer that had a similar hydrophobicity, retention time, and fragmentation patterns to that of cortisol found in eccrine sweat.”
From Wearable sensors for monitoring the physiological and biochemical profile of the athlete (2019) · cites this paper as [102]
Prior work to measure stress-related biomarkers has utilized high-performance liquid chromatography (HPLC), enzyme-linked immunosorbent assay, radioimmunoassay kit, or a HPLC mass spectrometry (MS) system.
“Prior work to measure stress-related biomarkers has utilized high-performance liquid chromatography (HPLC),99 enzyme-linked immunosorbent assay,100 radioimmunoassay kit,101 or a HPLC mass spectrometry (MS) system.102”
From Wearable sensors for monitoring the physiological and biochemical profile of the athlete (2019) · cites this paper as [102]
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- Authors
- Jia, M., Chew, W. M., Feinstein, Y., Skeath, P., & Sternberg, E. M.
- Affiliations
- University of Arizona (US)
- Corresponding author
- Jia, Min
- Sternberg, Esther M.
- Venue
- The Analyst
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2016
- Volume
- 141
- Issue
- 6
- Pages
- 2053-2060
- ISSN
- 0003-2654 (ISSN-L)
- 1364-5528
- PMC
- PMC5376102
- OpenAlex
- W2260554414
- Cited by
- 113
- References
- 32
- Citations by year
- 2026: 16 · 2025: 6 · 2024: 22 · 2023: 11 · 2022: 14 · 2021: 15 · 2020: 14 · 2019: 11 · 2018: 3 · 2017: 1
- FWCI
- 3.19
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc
- Locations
- The Analyst (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Air Force Research Laboratory (FA8650-13-2-7311)
- Topics
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Keywords
- cortisol, eccrine sweat, sweat biomarkers, stress biomarkers, non-invasive stress assessment, steroid quantification, atmospheric pressure chemical ionization, multiple reaction monitoring
- MeSH terms
- Chromatography, High Pressure Liquid, Exocrine Glands, Humans, Hydrocortisone, Sweat, Time Factors, Reproducibility of Results, Clinical Chemistry Tests, Analytic Sample Preparation Methods, Tandem Mass Spectrometry
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