The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Human macrophage activation programs induced by bacterial pathogens
Abstract
Understanding the response of innate immune cells to pathogens may provide insights to host defenses and the tactics used by pathogens to circumvent these defenses. We used DNA microarrays to explore the responses of human macrophages to a variety of bacteria. Macrophages responded to a broad range of bacteria with a robust, shared pattern of gene expression. The shared response includes genes encoding receptors, signal transduction molecules, and transcription factors. This shared activation program transforms the macrophage into a cell primed to interact with its environment and to mount an immune response. Further study revealed that the activation program is induced by bacterial components that are Toll-like receptor agonists, including lipopolysaccharide, lipoteichoic acid, muramyl dipeptide, and heat shock proteins. Pathogen-specific responses were also apparent in the macrophage expression profiles. Analysis of Mycobacterium tuberculosis -specific responses revealed inhibition of interleukin-12 production, suggesting one means by which this organism survives host defenses. These results improve our understanding of macrophage defenses, provide insights into mechanisms of pathogenesis, and suggest targets for therapeutic intervention.
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“Despite a number of genomic studies analyzing macrophage activation in response to bacteria, TLR ligands, and M1 or M2 stimuli, to date there have been no attempts to reconcile these observations by building new and integrative models of macrophage activation”
From Transcriptome-Based Network Analysis Reveals a Spectrum Model of Human Macrophage Activation (2014) · cites this paper as Nau et al., 2002
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Cluster analysis and display of genome-wide expression patterns
Eisen, M. B., Spellman, P. T., Brown, P. O., & Botstein, D. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.25.14863 · Cited by 16,184
Defective LPS Signaling in C3H/HeJ and C57BL/10ScCr Mice: Mutations in Tlr4 Gene
Poltorak, A., He, X., Smirnova, I., Liu, M.-Y., Huffel, C. V., Du, X., Birdwell, D., Alejos, E., Silva, M., Galanos, C., et al. (1998) · Science · doi:10.1126/science.282.5396.2085 · Cited by 7,631
Toll-like receptors: critical proteins linking innate and acquired immunity
Akira, S., Takeda, K., & Kaisho, T. (2001) · Nature Immunology · doi:10.1038/90609 · Cited by 4,777
Expression monitoring by hybridization to high-density oligonucleotide arrays
Lockhart, D. J., Dong, H., Byrne, M. C., Follettie, M. T., Gallo, M. V., Chee, M. S., Mittmann, M., Wang, C., Kobayashi, M., Norton, H., et al. (1996) · Nature Biotechnology · doi:10.1038/nbt1296-1675 · Cited by 2,841
Toll-like Receptor-4 Mediates Lipopolysaccharide-induced Signal Transduction
Chow, J. C., Young, D. W., Golenbock, D. T., Christ, W. J., & Gusovsky, F. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.16.10689 · Cited by 1,924
Peptidoglycan- and Lipoteichoic Acid-induced Cell Activation Is Mediated by Toll-like Receptor 2
Schwandner, R., Dziarski, R., Wesche, H., Rothe, M., & Kirschning, C. J. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.25.17406 · Cited by 1,704
Ohashi, K., Burkart, V., Flohé, S., & Kolb, H. (2000) · The Journal of Immunology · doi:10.4049/jimmunol.164.2.558 · Cited by 1,606
Yoshimura, A., Lien, E., Ingalls, R. R., Tuomanen, E., Dziarski, R., & Golenbock, D. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.163.1.1 · Cited by 1,234
Anderson, P. C. (1999) · JAMA · doi:10.1001/jama.281.24.2351 · Cited by 1,009
IL-12-Deficient Mice Are Defective in IFNγ Production and Type 1 Cytokine Responses
Magram, J., Connaughton, S. E., Warrier, R. R., Carvajal, D. M., Wu, C.-Y., Ferrante, J., Stewart, C., Sarmiento, U., Faherty, D. A., & Gately, M. K. (1996) · Immunity · doi:10.1016/s1074-7613(00)80413-6 · Cited by 989
Research works printed but not linked: 3
- 10.1379/1466-1268(2000)005<0401:IOAHPT>2.0.CO;2 · doi:10.1379/1466-1268(2000)005
- Goldstone S. E. Palefsky J. M. Winnett M. T. & Neefe J. R. (2002) Dis. Colon Rectum in press.
- S M Holland Adv Intern Med 45, 431–452 (2000).
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Alternative activation of macrophages
Gordon, S. (2003) · Nature Reviews Immunology · doi:10.1038/nri978 · Cited by 6,189
The M1 and M2 paradigm of macrophage activation: Time for reassessment
Martinez, F. O., & Gordon, S. (2014) · F1000Prime Reports · doi:10.12703/p6-13 · Cited by 4,689
Macrophages, inflammation, and insulin resistance
Olefsky, J. M., & Glass, C. K. (2010) · Annual Review of Physiology · doi:10.1146/annurev-physiol-021909-135846 · Cited by 2,733
Transcriptional regulation of macrophage polarization: enabling diversity with identity
Lawrence, T., & Natoli, G. (2011) · Nature Reviews Immunology · doi:10.1038/nri3088 · Cited by 2,284
Transcriptome-Based Network Analysis Reveals a Spectrum Model of Human Macrophage Activation
Xue, J., Schmidt, S. V., Sander, J., Draffehn, A., Krebs, W., Quester, I., De Nardo, D., Gohel, T. D., Emde, M., Schmidleithner, L., et al. (2014) · Immunity · doi:10.1016/j.immuni.2014.01.006 · Cited by 2,210
Mycobacterium tuberculosis Pathogenesis and Molecular Determinants of Virulence
Smith, I. (2003) · Clinical Microbiology Reviews · doi:10.1128/cmr.16.3.463-496.2003 · Cited by 1,154
Revollo, J. R., Grimm, A. A., & Imai, S.-I. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m408388200 · Cited by 1,010
TLR activation of the transcription factor XBP1 regulates innate immune responses in macrophages
Martinon, F., Chen, X., Lee, A.-H., & Glimcher, L. H. (2010) · Nature Immunology · doi:10.1038/ni.1857 · Cited by 996
“and after infection of human macrophages or mouse lung tissues with pathogenic Mycobacteria and Klebsiella pneumoniae specie”
BackgroundStanley, S. A., Raghavan, S., Hwang, W. W., & Cox, J. S. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2235593100 · Cited by 570
Macrophage reaction against biomaterials in the mouse model–phenotypes, functions and markers
Klopfleisch, R. (2016) · Acta Biomaterialia · doi:10.1016/j.actbio.2016.07.003 · Cited by 298
Litvak, V., Ramsey, S. A., Rust, A. G., Zak, D. E., Kennedy, K. A., Lampano, A. E., Nykter, M., Shmulevich, I., & Aderem, A. (2009) · Nature Immunology · doi:10.1038/ni.1721 · Cited by 295
Microglial phenotypes in Parkinson’s disease and animal models of the disease
Joers, V., Tansey, M. G., Mulas, G., & Carta, A. R. (2017) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2016.04.006 · Cited by 285
Agarwal, A. K., Rogers, P. D., Baerson, S. R., Jacob, M. R., Barker, K. S., Cleary, J. D., Walker, L. A., Nagle, D. G., & Clark, A. M. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m306291200 · Cited by 204
Sirt3 promotes antimycobacterial defenses by coordinating mitochondrial and autophagic functions
Kim, T. S., Jin, Y. B., Kim, Y. S., Kim, S., Kim, J. K., Lee, H.-M., Suh, H.-W., Choe, J. H., Kim, Y. J., Koo, B.-S., et al. (2019) · Autophagy · doi:10.1080/15548627.2019.1582743 · Cited by 172
The genomic landscapes of inflammation
Natoli, G., Ghisletti, S., & Barozzi, I. (2011) · Genes & Development · doi:10.1101/gad.2018811 · Cited by 152
Ramsey, S. A., Klemm, S. L., Zak, D. E., Kennedy, K. A., Thorsson, V., Li, B., Gilchrist, M., Gold, E. S., Johnson, C. D., Litvak, V., et al. (2008) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1000021 · Cited by 147
Wurfel, M. M., Park, W. Y., Radella, F., Ruzinski, J., Sandstrom, A., Strout, J., Bumgarner, R. E., & Martin, T. R. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.175.4.2570 · Cited by 130
Kurtz, S., McKinnon, K. P., Runge, M. S., Ting, J. P.-Y., & Braunstein, M. (2006) · Infection and Immunity · doi:10.1128/iai.01022-06 · Cited by 114
Neves, A. R., Castelo-Branco, M. T. L., Figliuolo, V. R., Bernardazzi, C., Buongusto, F., Yoshimoto, A., Nanini, H. F., Coutinho, C. M. L. M., Carneiro, A. J. V., Coutinho-Silva, R., et al. (2014) · Inflammatory Bowel Diseases · doi:10.1097/01.mib.0000441201.10454.06 · Cited by 107
Prophylactic systemic P2X7 receptor blockade prevents experimental colitis
Marques, C. C., Castelo-Branco, M. T., Pacheco, R. G., Buongusto, F., do Rosário, A., Schanaider, A., Coutinho-Silva, R., & de Souza, H. S. P. (2014) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2013.10.012 · Cited by 78
Interaction of Chlamydiae with human macrophages
Herweg, J., & Rudel, T. (2016) · The FEBS Journal · doi:10.1111/febs.13609 · Cited by 39
Gekonge, B., Giri, M. S., Kossenkov, A. V., Nebozyhn, M., Yousef, M., Mounzer, K., Showe, L., & Montaner, L. J. (2012) · PLoS ONE · doi:10.1371/journal.pone.0041153 · Cited by 21
Metadata
- Authors
- Nau, G. J., Richmond, J. F. L., Schlesinger, A., Jennings, E. G., Lander, E. S., & Young, R. A.
- Affiliations
- Whitehead Institute, 9 Cambridge Center, Cambridge, MA 02142; Infectious Disease Unit, Massachusetts General Hospital, Boston, MA 02114; and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2002-01-22
- Volume
- 99
- Issue
- 3
- Pages
- 1503-1508
- ISSN
- 0027-8424
- 1091-6490
- Cited by
- 568
- References
- 32
- Language
- en
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