The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Formation of new chromosomes as a virulence mechanism in yeast Candida glabrata
Open accessAbstract
In eukaryotes, the number and rough organization of chromosomes is well preserved within isolates of the same species. Novel chromosomes and loss of chromosomes are infrequent and usually associated with pathological events. Here, we analyzed 40 pathogenic isolates of a haploid and asexual yeast, Candida glabrata, for their genome structure and stability. This organism has recently become the second most prevalent yeast pathogen in humans. Although the gene sequences were well conserved among different strains, their chromosome structures differed drastically. The most frequent events reshaping chromosomes were translocations of chromosomal arms. However, also larger segmental duplications were frequent and occasionally we observed novel chromosomes. Apparently, this yeast can generate a new chromosome by duplication of chromosome segments carrying a centromere and subsequently adding novel telomeric ends. We show that the observed genome plasticity is connected with antifungal drug resistance and it is likely an advantage in the human body, where environmental conditions fluctuate a lot.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Previous studies found that C. glabrata can undergo genomic re-arrangements as an adaptive survival mechanism (Shin et al., 2007; Muller et al., 2009; Poláková et al., 2009).”
From Genome Comparisons of Candida glabrata Serial Clinical Isolates Reveal Patterns of Genetic Variation in Infecting Clonal Populations (2019) · cites this paper as Poláková et al., 2009
In the citing paper
“According to Poláková et al. [90], the genetic instability results in segmental duplications, chromosomal rearrangements, and extra chromosomes occurring in C. glabrata at high frequency.”
From Candida glabrata: pathogenicity and resistance mechanisms for adaptation and survival (2021) · cites this paper as [90]
In the citing paper
“Duplicated segments of ChrFL encode a transporter of the ATP-binding cassette family (CAGL0F01419g) that is very similar to S. cerevisiae AUS1.”
From Candida glabrata: pathogenicity and resistance mechanisms for adaptation and survival (2021) · cites this paper as [90]
In the citing paper
“Aneuploidy gain of small chromosome segment on the left arm of chromosome F that encodes ABC transporter AUS1 and PDH1 is also observed in C. glabrata-resistant isolates [90].”
From Candida glabrata: pathogenicity and resistance mechanisms for adaptation and survival (2021) · cites this paper as [90]
In the citing paper
“The small chromosome F encodes an ortholog of S. cerevisiae ABC transporter PDR5 (CAGL0F02717g) known in C. glabrata as PDH1.”
From Candida glabrata: pathogenicity and resistance mechanisms for adaptation and survival (2021) · cites this paper as [90]
In the citing paper
“Several genes on chromosomes (ChrEL and ChrFL) potentially mediate interactions between C. glabrata and the susceptible host.”
From Candida glabrata: pathogenicity and resistance mechanisms for adaptation and survival (2021) · cites this paper as [90]
In the citing paper
“For the haploid yeast C. glabrata, genome rearrangements underlying azole resistance include chromosomal translocations, segmental duplications, and occasionally, the formation of de novo minichromosomes70.”
From Molecular mechanisms governing antifungal drug resistance (2023) · cites this paper as [70]
In the citing paper
“Since genome plasticity has been connected with antifungal drug resistance in C. glabrata (Shin et al., 2007; Polakova et al., 2009)”
From Genetic Drivers of Multidrug Resistance in Candida glabrata (2016) · cites this paper as Polakova et al., 2009
In the citing paper
“the most frequent genome rearrangements identified were translocations of chromosomal arms and inter-chromosomal duplications, but formation of novel chromosomes was also reported (Muller et al., 2009; Polakova et al., 2009).”
From Genetic Drivers of Multidrug Resistance in Candida glabrata (2016) · cites this paper as Polakova et al., 2009
In the citing paper
“Karyotyping using pulsed-field gel electrophoresis (PFGE), which separates whole chromosomes, revealed that C. glabrata genome organization varies significantly among different isolates (Muller et al., 2009; Polakova et al., 2009).”
From Genetic Drivers of Multidrug Resistance in Candida glabrata (2016) · cites this paper as Polakova et al., 2009
In the citing paper
“Indeed, while the genome of a reference C. glabrata laboratory strain (CBS138) has been sequenced, we were not able to use that strain's genomic sequence as a template for assembling the sequences of other strains, e.g., various clinical isolates, due to the high karyotypic variability among strains (Muller et al., 2009; Polakova et al., 2009; Bader et al., 2012; Figure 1B).”
From Genetic Drivers of Multidrug Resistance in Candida glabrata (2016) · cites this paper as Polakova et al., 2009
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
Saitou, N., & Nei, M. (1987) · Molecular Biology and Evolution · doi:10.1093/oxfordjournals.molbev.a040454 · Cited by 59,959
Thompson, J. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.24.4876 · Cited by 38,685
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0
Tamura, K., Dudley, J. T., Nei, M., & Kumar, S. (2007) · Molecular Biology and Evolution · doi:10.1093/molbev/msm092 · Cited by 28,734
Prospects for inferring very large phylogenies by using the neighbor-joining method
Tamura, K., Nei, M., & Kumar, S. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0404206101 · Cited by 5,588
The Significance of Responses of the Genome to Challenge
McClintock, B. (1984) · Science · doi:10.1126/science.15739260 · Cited by 2,638
Dujon, B., Sherman, D., Fischer, G., Durrens, P., Casaregola, S., Lafontaine, I., de Montigny, J., Marck, C., Neuvéglise, C., Talla, E., et al. (2004) · Nature · doi:10.1038/nature02579 · Cited by 1,623
Effects of Aneuploidy on Cellular Physiology and Cell Division in Haploid Yeast
Torres, E. M., Sokolsky, T., Tucker, C. M., Chan, L. Y., Boselli, M., Dunham, M. J., & Amon, A. (2007) · Science · doi:10.1126/science.1142210 · Cited by 935
Phylogenetic test of the molecular clock and linearized trees.
Takezaki, N., Rzhetsky, A., & Nei, M. (1995) · Molecular Biology and Evolution · doi:10.1093/oxfordjournals.molbev.a040259 · Cited by 826
Potential Role of Phospholipases in Virulence and Fungal Pathogenesis
Ghannoum, M. A. (2000) · Clinical Microbiology Reviews · doi:10.1128/cmr.13.1.122-143.2000 · Cited by 823
Aneuploidy and Isochromosome Formation in Drug-Resistant Candida albicans
Selmecki, A., Forche, A., & Berman, J. (2006) · Science · doi:10.1126/science.1128242 · Cited by 733
Wotton, D., & Shore, D. (1997) · Genes & Development · doi:10.1101/gad.11.6.748 · Cited by 412
Candida albicans proteinases and host/pathogen interactions
Naglik, J. R., Albrecht, A., Bader, O., & Hube, B. (2004) · Cellular Microbiology · doi:10.1111/j.1462-5822.2004.00439.x · Cited by 402
Break-Induced Replication and Recombinational Telomere Elongation in Yeast
McEachern, M. J., & Haber, J. E. (2006) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.74.082803.133234 · Cited by 311
Chromosomal evolution in Saccharomyces
Fischer, G., James, S. A., Roberts, I. N., Oliver, S. G., & Louis, E. J. (2000) · Nature · doi:10.1038/35013058 · Cited by 304
[11] DNA of Saccharomyces cerevisiae
Philippsen, P., Stotz, A., & Scherf, C. (1991) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(91)94014-4 · Cited by 302
Kaur, R., Ma, B., & Cormack, B. P. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0611195104 · Cited by 284
Teng, S., Chang, J. Y., McCowan, B., & Zakian, V. A. (2000) · Molecular Cell · doi:10.1016/s1097-2765(05)00094-8 · Cited by 280
Ten1 functions in telomere end protection and length regulation in association with Stn1 and Cdc13
Grandin, N., Damon‐Soubeyrand, C., & Charbonneau, M. (2001) · The EMBO Journal · doi:10.1093/emboj/20.5.1173 · Cited by 249
Engineering evolution to study speciation in yeasts
Delneri, D., Colson, I., Grammenoudi, S., Roberts, I. N., Louis, E. J., & Oliver, S. G. (2003) · Nature · doi:10.1038/nature01418 · Cited by 242
Circular chromosome formation in a fission yeast mutant defective in two ATM homologues
Naito, T., Matsuura, A., & Ishikawa, F. (1998) · Nature Genetics · doi:10.1038/2517 · Cited by 237
Eucaryotic genome evolution through the spontaneous duplication of large chromosomal segments
Koszul, R., Caburet, S., Dujon, B., & Fischer, G. (2003) · The EMBO Journal · doi:10.1038/sj.emboj.7600024 · Cited by 227
GENE AMPLIFICATION IN LEISHMANIA
Beverley, S. M. (1991) · Annual Review of Microbiology · doi:10.1146/annurev.mi.45.100191.002221 · Cited by 217
Carbon Assimilation Tests for the Classification of Yeasts
Wickerham, L. J., & Burton, K. A. (1948) · Journal of Bacteriology · doi:10.1128/jb.56.3.363-371.1948 · Cited by 211
Telomere length control and transcriptional regulation of subtelomeric adhesins in Candida glabrata
Castaño, I., Pan, S., Zupancic, M., Hennequin, C., Dujon, B., & Cormack, B. P. (2005) · Molecular Microbiology · doi:10.1111/j.1365-2958.2004.04465.x · Cited by 188
Ubeda, J.-M., Légaré, D., Raymond, F., Ouameur, A. A., Boisvert, S., Rigault, P., Corbeil, J., Tremblay, M. J., Olivier, M., Papadopoulou, B., et al. (2008) · Genome biology · doi:10.1186/gb-2008-9-7-r115 · Cited by 181
Sharpless, N. E., Ferguson, D. O., O’Hagan, R. C., Castrillón, D. H., Lee, C., Farazi, P. A., Alson, S., Fleming, J., Morton, C. C., Frank, K. M., et al. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00425-7 · Cited by 173
Reintroduction of the PLB1 gene into Candida albicans restores virulence in vivo
Mukherjee, P. K., Seshan, K. R., Leidich, S. D., Chandra, J., Cole, G. T., & Ghannoum, M. A. (2001) · Microbiology · doi:10.1099/00221287-147-9-2585 · Cited by 100
Shin, J. H., Chae, M. J., Song, J. W., Jung, S.-I., Cho, D., Kee, S. J., Kim, S. H., Shin, M. G., Suh, S. P., & Ryang, D. W. (2007) · Journal of Clinical Microbiology · doi:10.1128/jcm.00381-07 · Cited by 86
Lin, C.-Y., Chen, Y.-C., Lo, H.-J., Chen, K.-W., & Li, S.-Y. (2007) · Journal of Clinical Microbiology · doi:10.1128/jcm.00699-07 · Cited by 62
Karyotypes of Saccharomyces sensu lato species
Petersen, R. F., Nilsson‐Tillgren, T., & Piškur, J. (1999) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/00207713-49-4-1925 · Cited by 49
Thierry, A., Bouchier, C., Dujon, B., & Richard, G.-F. (2008) · Nucleic Acids Research · doi:10.1093/nar/gkn594 · Cited by 45
Chromosomal translocations in leukaemia.
Gauwerky, C. E., & Croce, C. M. (1993) · PubMed · Cited by 42
Karyotyping of Candida albicans and Candida glabrata from patients with Candida sepsis
Klempp‐Selb, Rimek, & Kappe (2000) · Mycoses · doi:10.1046/j.1439-0507.2000.00555.x · Cited by 35
Transfer of Genetic Material between Pathogenic and Food-Borne Yeasts
Mentel, M., Špı́rek, M., Jørck-Ramberg, D., & Piškur, J. (2006) · Applied and Environmental Microbiology · doi:10.1128/aem.00293-06 · Cited by 19
Haw, R., Yarragudi, A., & Uemura, H. (2001) · Yeast · doi:10.1002/yea.775 · Cited by 18
Flagged first, then by citations
Research works printed but not linked: 2
- CE Gauwerky, CM Croce, Chromosomal translocations in leukemia. Semin Cancer Biol 4, 333–340 (1993).
- 10.1128/CMR.13.1.122 · DOI
Cited by
Sorted by citations
Antifungal Drug Resistance: Molecular Mechanisms in Candida albicans and Beyond
Lee, Y., Puumala, E., Robbins, N., & Cowen, L. E. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00199 · Cited by 801
Mechanisms of Antifungal Drug Resistance
Cowen, L. E., Sanglard, D., Howard, S. J., Rogers, P. D., & Perlin, D. S. (2015) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a019752 · Cited by 647
Aneuploidy confers quantitative proteome changes and phenotypic variation in budding yeast
Pavelka, N., Rancati, G., Zhu, J., Bradford, W. D., Saraf, A., Florens, L., Sanderson, B. W., Hattem, G. L., & Li, R. (2010) · Nature · doi:10.1038/nature09529 · Cited by 577
Fluconazole resistance in Candida species: a current perspective
Berkow, E., & Lockhart, S. (2017) · Infection and Drug Resistance · doi:10.2147/idr.s118892 · Cited by 542
Sionov, E., Lee, H., Chang, Y. C., & Kwon-Chung, K. J. (2010) · PLoS Pathogens · doi:10.1371/journal.ppat.1000848 · Cited by 479
Metadata
- Authors
- Poláková, S., Blume, C., Zárate, J. Á., Mentel, M., Jørck-Ramberg, D., Stenderup, J., & Piškur, J.
- Affiliations
- Lund University (SE)
- Technical University of Denmark (DK)
- Regionshospitalet Herning (DK)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2009-02-24
- Volume
- 106
- Issue
- 8
- Pages
- 2688-2693
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC2637908
- OpenAlex
- W2105649156
- Cited by
- 206
- References
- 35
- Citations by year
- 2026: 2 · 2025: 9 · 2024: 12 · 2023: 10 · 2022: 12 · 2021: 11 · 2020: 11 · 2019: 9 · 2018: 13 · 2017: 8 · 2016: 7 · 2015: 13 · 2014: 15 · 2013: 14 · 2012: 15
- FWCI
- 8.04
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Lund University Publications (Lund University) (submittedVersion)
- Technical University of Denmark, DTU Orbit (Technical University of Denmark, DTU) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Immunology and Microbiology › Microbiology › Yeasts and Rust Fungi Studies
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Keywords
- Candida glabrata, chromosomal translocation, segmental duplication, genome plasticity, antifungal resistance, virulence, telomeres, centromere, haploid yeast, asexual reproduction, fungal pathogens
- MeSH terms
- Antifungal Agents, Base Sequence, Phylogeny, Virulence, Blotting, Southern, Chromosomes, Fungal, Polymerase Chain Reaction, Electrophoresis, Gel, Pulsed-Field, DNA Primers, Candida glabrata
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
