Short actin-based mechanism for light-directed chloroplast movement in Arabidopsis
Open accessAbstract
Organelle movement is essential for proper function of living cells. In plants, these movements generally depend on actin filaments, but the underlying mechanism is unknown. Here, in Arabidopsis , we identify associations of short actin filaments along the chloroplast periphery on the plasma membrane side associated with chloroplast photorelocation and anchoring to the plasma membrane. We have termed these chloroplast-actin filaments (cp-actin filaments). Cp-actin filaments emerge from the chloroplast edge and exhibit rapid turnover. The presence of cp-actin filaments depends on an actin-binding protein, chloroplast unusual positioning1 (CHUP1), localized on the chloroplast envelope. chup1 mutant lacked cp-actin filaments but showed normal cytoplasmic actin filaments. When irradiated with blue light to induce chloroplast movement, cp-actin filaments relocalize to the leading edge of chloroplasts before and during photorelocation and are regulated by 2 phototropins, phot1 and phot2. Our findings suggest that plants evolved a unique actin-based mechanism for organelle movement.
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CP-actin polymerization generates force for chloroplast movement.
“studies have suggested that CP-actin polymerization generates force”
From The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants (2022) · cites this paper as Kadota et al., 2009
The Results (page unavailable) suggest that biased cp-actin filaments may be needed to generate the motive force for chloroplast movement. The Discussion (page unavailable) reports that these filaments polymerize, but says further analysis is needed to understand how they generate force; it does not specifically establish polymerization as the force-generating mechanism. (LLM, conf=0.84)
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“Although the mechanism by which chloroplast movement generates force is unclear, studies have suggested that CP-actin polymerization generates force (Kadota et al., 2009) (Fig.”
BackgroundWang, J., Liang, Y.-P., Zhu, J.-D., Wang, Y.-X., Yang, M.-Y., Yan, H.-R., Lv, Q.-Y., Cheng, K., Zhao, X., & Zhang, X. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.704618 · Cited by 15 · see citing works
- Authors
- Kadota, A., Yamada, N., Suetsugu, N., Hirose, M., Saito, C., Shoda, K., Ichikawa, S., Kagawa, T., Nakano, A., & Wada, M.
- Affiliations
- Tokyo Metropolitan University (JP)
- Kyushu University (JP)
- National Institute for Basic Biology (JP)
- RIKEN (JP)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2009-08-04
- Volume
- 106
- Issue
- 31
- Pages
- 13106-13111
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC2722281
- OpenAlex
- W2080614820
- Cited by
- 208
- References
- 27
- Citations by year
- 2026: 9 · 2025: 8 · 2024: 5 · 2023: 11 · 2022: 8 · 2021: 7 · 2020: 11 · 2019: 8 · 2018: 13 · 2017: 11 · 2016: 14 · 2015: 14 · 2014: 7 · 2013: 13 · 2012: 17
- FWCI
- 27.87
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (20227001)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- chloroplast movement, F-actin, phototropin, phot1, phot2, blue light signaling, organelle motility, Arabidopsis thaliana, chloroplast envelope
- MeSH terms
- Actins, Cell Membrane, Chloroplasts, Flavoproteins, Fluorescence, Light, Microfilament Proteins, Microtubules, Movement, Arabidopsis, Arabidopsis Proteins, Green Fluorescent Proteins, Cryptochromes, Chloroplast Proteins
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