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Cited by 179

The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.

Genome-wide analysis reveals methyl-CpG–binding protein 2–dependent regulation of microRNAs in a mouse model of Rett syndrome

Open access
Wu, Hao, Tao, Jifang, Chen, Pauline J., Shahab, Atif, Ge, Weihong, Hart, Ronald P., Ruan, Xiaoan, Ruan, Yijun, Sun, Yi E.·2010·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.1005595107
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Abstract

MicroRNAs (miRNAs) are a class of small, noncoding RNAs that function as posttranscriptional regulators of gene expression. Many miRNAs are expressed in the developing brain and regulate multiple aspects of neural development, including neurogenesis, dendritogenesis, and synapse formation. Rett syndrome (RTT) is a progressive neurodevelopmental disorder caused by mutations in the gene encoding methyl-CpG–binding protein 2 ( MECP2 ). Although Mecp2 is known to act as a global transcriptional regulator, miRNAs that are directly regulated by Mecp2 in the brain are not known. Using massively parallel sequencing methods, we have identified miRNAs whose expression is altered in cerebella of Mecp2 -null mice before and after the onset of severe neurological symptoms. In vivo genome-wide analyses indicate that promoter regions of a significant fraction of dysregulated miRNA transcripts, including a large polycistronic cluster of brain-specific miRNAs, are DNA-methylated and are bound directly by Mecp2. Functional analysis demonstrates that the 3′ UTR of messenger RNA encoding Brain-derived neurotrophic factor ( Bdnf ) can be targeted by multiple miRNAs aberrantly up-regulated in the absence of Mecp2. Taken together, these results suggest that dysregulation of miRNAs may contribute to RTT pathoetiology and also may provide a valuable resource for further investigations of the role of miRNAs in RTT.

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Research works printed but not linked: 24

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Metadata

Authors
Wu, H., Tao, J., Chen, P. J., Shahab, A., Ge, W., Hart, R. P., Ruan, X., Ruan, Y., & Sun, Y. E.
Venue
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Published
2010-10-04
Volume
107
Issue
42
Pages
18161-18166
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.1005595107
OpenAlex
W2013405241
Cited by
179
References
23
FWCI
9.05
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Genome-wide analysis reveals methyl-CpG–binding protein 2–dependent regulation of microRNAs in a mouse model of Rett syndrome. https://app.cochat.ai/papers/10.1073/pnas.1005595107
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Wu, H., Tao, J., Chen, P. J., Shahab, A., Ge, W., Hart, R. P., Ruan, X., Ruan, Y., & Sun, Y. E. (2010). Genome-wide analysis reveals methyl-CpG–binding protein 2–dependent regulation of microRNAs in a mouse model of Rett syndrome. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1005595107

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