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MicroRNA-92b regulates the development of intermediate cortical progenitors in embryonic mouse brain

Nowakowski, Tomasz Jan, Fotaki, Vassiliki, Pollock, Andrew, Sun, Tao, Pratt, Thomas, Price, David J.·2013·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.1219385110
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Abstract

Cerebral cortical neurons arise from radial glia (direct neurogenesis) or from intermediate progenitors (indirect neurogenesis); intriguingly, the sizes of intermediate progenitor populations and the cortices they generate correlate across species. The generation of intermediate progenitors is regulated by the transcription factor Tbr2, whose expression marks these cells. We investigated how this mechanism might be controlled. We found that acute blockade of mature microRNA biosynthesis in murine cortical progenitors caused a rapid cell autonomous increase in numbers of Tbr2-expressing cells. Acute microRNA-92b (miR-92b) gain of function caused rapid reductions in numbers of Tbr2-expressing cells and proliferating intermediate progenitors. Acute miR-92b loss of function had opposite effects. These findings indicate that miR-92b limits the production of intermediate cortical progenitors.

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Cited by

Sorted by citations

Authors
Nowakowski, T. J., Fotaki, V., Pollock, A., Sun, T., Pratt, T., & Price, D. J.
Affiliations
  • Centre for Integrative Physiology, University of Edinburgh, Edinburgh EH8 9XD, United Kingdom; and
  • Department of Cell and Developmental Biology, Cornell University Weill Medical College, New York, NY 10021
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2013-04-08
Volume
110
Issue
17
Pages
7056-7061
ISSN
  • 0027-8424
  • 1091-6490
DOI
10.1073/pnas.1219385110
Cited by
95
References
3
Language
en

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