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Temperate and lytic bacteriophages programmed to sensitize and kill antibiotic-resistant bacteria
Abstract
Significance: Antibiotic resistance of pathogens is a growing concern to human health, reviving interest in phage therapy. This therapy uses phages (natural bacterial enemies) to kill pathogens. However, it encounters many obstacles such as delivery barriers into the tissues and bacterial resistance to phages. Here, we use phages for delivering a programmable DNA nuclease, clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas), to reverse antibiotic resistance and eliminate the transfer of resistance between strains. This approach combines CRISPR-Cas delivery with lytic phage selection of antibiotic-sensitized bacteria. The strategy may reduce the prevalence of antibiotic-resistant bacteria in treated surfaces and on skin of medical personnel, as it uses phages in a unique way that overcomes many of the hurdles encountered by phage therapy.
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Yosef et al. developed technology with lytic phage to selectively kill multi-drug-resistant bacteria.
“Later, Yosef et al. further developed this technology with lytic phage to selectively kill multi-drug-resistance bacteria [61].”
From Viral Delivery Systems for CRISPR (2019) · cites this paper as [61]
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Multiplex Genome Engineering Using CRISPR/Cas Systems
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RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
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CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
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RNA-guided editing of bacterial genomes using CRISPR-Cas systems
Jiang, W., Bikard, D., Cox, D., Zhang, F., & Marraffini, L. A. (2013) · Nature Biotechnology · doi:10.1038/nbt.2508 · Cited by 2,429
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Engineered phage with antibacterial CRISPR–Cas selectively reduce E. coli burden in mice
Gencay, Y. E., Jasinskytė, D., Robert, C., Semsey, S., Martínez, V., Petersen, A. Ø., Brunner, K., de Santiago Torio, A., Salazar, A., Turcu, I. C., et al. (2024) · Nature Biotechnology · doi:10.1038/s41587-023-01759-y · Cited by 282
Viral Delivery Systems for CRISPR
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High-throughput mapping of the phage resistance landscape in E. coli
Mutalik, V. K., Adler, B. A., Rishi, H. S., Piya, D., Zhong, C., Koskella, B., Kutter, E. M., Calendar, R., Novichkov, P. S., Price, M. N., et al. (2020) · PLOS Biology · doi:10.1371/journal.pbio.3000877 · Cited by 171
The application of the CRISPR-Cas system in antibiotic resistance
Tao, S., Chen, H., Li, N., & Liang, W. (2022) · Infection and Drug Resistance · doi:10.2147/idr.s370869 · Cited by 147
Intestinal phages interact with bacteria and are involved in human diseases
Shuwen, H., & Kefeng, D. (2022) · Gut Microbes · doi:10.1080/19490976.2022.2113717 · Cited by 124
Targeting friend and foe: Emerging therapeutics in the age of gut microbiome and disease
Cho, J. A., & Chinnapen, D. J. F. (2018) · Journal of Microbiology · doi:10.1007/s12275-018-8037-z · Cited by 19
Metadata
- Authors
- Yosef, I., Manor, M., Kiro, R., & Qimron, U.
- Affiliations
- Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
- ORCID
- Qimron, Udi
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2015-05-18
- Volume
- 112
- Issue
- 23
- Pages
- 7267-7272
- ISSN
- 0027-8424
- 1091-6490
- Cited by
- 483
- References
- 5
- Language
- en
- Funders
- Israel Science Foundation (268/14)
- EC | European Research Council (336079)
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