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CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea

Marraffini, Luciano A., Sontheimer, Erik J.·2010·Nature Reviews Genetics
DOI 10.1038/nrg2749PMC PMC2928866
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  • In all types of CRISPR-Cas systems, the CRISPR locus is transcribed into a crRNA precursor (pre-crRNA), which is subsequently cleaved and processed by Cas proteins or cellular ribonucleases, yielding smaller units of mature crRNA.

    “In all types of CRISPR-Cas systems, the CRISPR locus is transcribed into a crRNA precursor (pre-crRNA), which is subsequently cleaved and processed by Cas proteins or cellular ribonucleases, yielding smaller units of mature crRNA [32].”

    From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [32]

    The ‘CRISPR transcription and processing’ section describes transcription of CRISPR arrays into a long precursor and Cas-mediated cleavage into small crRNAs. It does not establish this pathway for all CRISPR–Cas types; the authors explicitly ask how broadly their processing conclusions extend across subtypes. The section also does not identify cellular ribonucleases as an alternative to Cas proteins for this cleavage. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

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Research works printed but not linked: 88

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Metadata

Authors
Marraffini, L. A., & Sontheimer, E. J.
Venue
Nature Reviews Genetics
Publisher
Springer Science and Business Media LLC
Published
2010-02-02
Volume
11
Issue
3
Pages
181-190
ISSN
  • 1471-0056
  • 1471-0064
DOI
10.1038/nrg2749
PMC
PMC2928866
Cited by
919
References
0
Language
en
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