Dnmt3a restrains mast cell inflammatory responses
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Significance Methylation of genomic DNA is an epigenetic modification at the interface between genetic information and environmental stimuli underlying many phenotypic variations in the human population as well as the pathogenesis of complex diseases. Accordingly, mutations in the de novo DNA methyltransferase enzyme DNMT3A have been identified in a number of diseases, including mast cell-related disorders. However, the role of DNA methylation and DNMT3A in regulating mast cell physiology still needs to be elucidated. Here, we found that Dnmt3a plays a critical role in modulating mast cell responsiveness to acute and chronic stimulation, potentially implicating DNA methylation-mediated processes in all types of mast cell-related diseases.
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Enhanced mast cell activity was seen in a mouse model of allergy.
“enhanced mast cell activity was also seen in a mouse model of allergy 94”
From Clonal hematopoiesis in human aging and disease (2019) · cites this paper as [94]
Mast cells from mice that lacked Dnmt3a produced higher levels of IL-6, TNF-alpha, and IL-13 in response to stimulation with IgE in vitro.
“one study found that mast cells from mice that lacked Dnmt3a produced higher levels of IL-6, TNF-alpha, and IL-13 in response to stimulation with IgE in vitro”
From Clonal hematopoiesis in human aging and disease (2019) · cites this paper as [94]
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- Authors
- Leoni, C., Montagner, S., Rinaldi, A., Bertoni, F., Polletti, S., Balestrieri, C., & Monticelli, S.
- Affiliations
- University of Bern (CH)
- Institute for Research in Biomedicine (CH)
- Institute of Oncology Research (CH)
- European Institute of Oncology (IT)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2017-02-06
- Volume
- 114
- Issue
- 8
- Pages
- E1490-E1499
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC5338402
- OpenAlex
- W2587262629
- Cited by
- 138
- References
- 72
- Citations by year
- 2026: 2 · 2025: 17 · 2024: 17 · 2023: 17 · 2022: 19 · 2021: 16 · 2020: 20 · 2019: 13 · 2018: 11 · 2017: 6
- FWCI
- 6.19
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Swiss National Science Foundation (156875, 164489)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
- Keywords
- DNMT3A, DNA methylation, mast cells, epigenetic regulation, de novo DNA methyltransferases
- MeSH terms
- DNA (Cytosine-5-)-Methyltransferase 1, Decitabine, DNA Methyltransferase 3A, Animals, Azacitidine, Cells, Cultured, Dermatitis, Contact, Disease Models, Animal, DNA (Cytosine-5-)-Methyltransferases, Enzyme-Linked Immunosorbent Assay, Female, Fluorescent Antibody Technique, Immunoglobulin E, Interleukin-3, Mast Cells, Mutation, Oxazolone, Passive Cutaneous Anaphylaxis, Down-Regulation, Cell Degranulation, Mice, Knockout, DNA Methylation, ras GTPase-Activating Proteins, RNA Interference, Mastocytosis, Systemic, RNA, Small Interfering, Epigenesis, Genetic, Cell Proliferation, Mice
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