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Cited by 350Redox biology and oxidative stressNitric Oxide and Endothelin EffectsNeutrophil, Myeloperoxidase and Oxidative Mechanisms

Proteomic method identifies proteins nitrated in vivo during inflammatory challenge

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Aulak, Kulwant S., Miyagi, Masaru, Yan, Lin, West, Karen A., Massillon, Duna, Crabb, John W., Stuehr, Dennis J.·2001·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.221269198PMC PMC59826
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Abstract

Inflammation in asthma, sepsis, transplant rejection, and many neurodegenerative diseases associates an up-regulation of NO synthesis with increased protein nitration at tyrosine. Nitration can cause protein dysfunction and is implicated in pathogenesis, but few proteins that appear nitrated in vivo have been identified. To understand how this modification impacts physiology and disease, we used a proteomic approach toward targets of protein nitration in both in vivo and cell culture inflammatory disease models. This approach identified more than 40 nitrotyrosine-immunopositive proteins, including 30 not previously identified, that became modified as a consequence of the inflammatory response. These targets include proteins involved in oxidative stress, apoptosis, ATP production, and other metabolic functions. Our approach provides a means toward obtaining a comprehensive view of the nitroproteome and promises to broaden understanding of how NO regulates cellular processes.

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  • Multiple studies have shown that nitration is a selective process.

    “Although a growing number of proteins have now been shown to be nitrated, multiple studies have shown that nitration is a selective process (47, 484).”

    From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [47]

  • The nitroproteome appears to be quite specific.

    “Interestingly, the nitroproteome appears to be quite specific, and pathological responses may be due to modification of particularly sensitive proteins, including those that regulate signaling and cell metabolism (49–52).”

    From What Part of NO Don't You Understand? Some Answers to the Cardinal Questions in Nitric Oxide Biology (2010) · cites this paper as [52]

  • Pathological responses may be due to modification of particularly sensitive proteins, including those that regulate signaling and cell metabolism.

    “Interestingly, the nitroproteome appears to be quite specific, and pathological responses may be due to modification of particularly sensitive proteins, including those that regulate signaling and cell metabolism (49–52).”

    From What Part of NO Don't You Understand? Some Answers to the Cardinal Questions in Nitric Oxide Biology (2010) · cites this paper as [52]

  • Peroxynitrite and reactive species derived from it are important in inflammatory responses and can modulate cell signaling, in part by promoting oxidation or nitration of biomolecules.

    “Peroxynitrite and reactive species derived from it (e.g., nitrogen dioxide) are important in inflammatory responses [85] and can modulate cell signaling [87-90], in part by promoting the oxidation or nitration of a broad range of biomolecules [62].”

    From Regulation of obesity and insulin resistance by nitric oxide (2014) · cites this paper as [90]

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Research works printed but not linked: 6

  1. [10]Schmidt H H W. Biochemica. 1995;2:22. [Google Scholar]
  2. [11]West, K. A., Yan, L., Miyagi, M., Crabb, J. S., Murmorstein, A. D. Murmorstein, L. & Crabb, J. W. (2001) Exp. Eye Res., in press. [DOI] [PubMed]
  3. [32]Castro L, Rodriguez M, Radi R. J Biol Chem. 1994;269:29409–29415. [PubMed] [Google Scholar]
  4. [41]Piszkiewicz D, Landon M, Smith E L. J Biol Chem. 1971;246:1324–1329. [PubMed] [Google Scholar]
  5. [43]Blumenthal K M, Smith E L. J Biol Chem. 1975;250:6560–6563. [PubMed] [Google Scholar]
  6. [44]Jeckel D, Anders R, Pfleiderer G. Hoppe-Seyler's Z Physiol Chem. 1971;352:769–779. [PubMed] [Google Scholar]

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Authors
Aulak, K. S., Miyagi, M., Yan, L., West, K. A., Massillon, D., Crabb, J. W., & Stuehr, D. J.
Affiliations
Cleveland Clinic (US)
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2001-10-02
Volume
98
Issue
21
Pages
12056-12061
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.221269198
PMC
PMC59826
OpenAlex
W1980805550
Cited by
350
References
51
Citations by year
2026: 2 · 2025: 1 · 2024: 4 · 2023: 5 · 2022: 1 · 2021: 3 · 2020: 6 · 2019: 3 · 2018: 6 · 2017: 9 · 2016: 13 · 2015: 8 · 2014: 14 · 2013: 12 · 2012: 14
FWCI
10.26
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • American Heart Association
  • Cleveland Clinic
  • Cleveland Clinic Foundation
  • National Institutes of Health (5r21ns041644-02, CA53914, EY06603)
  • National Eye Institute (EY06603, F32EY006603, R01EY006603)
  • National Cancer Institute (CA-53914, R01CA053914)
  • National Institute of General Medical Sciences (GM51491, R01GM051491)
  • National Institute of Neurological Disorders and Stroke (NS41644)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Keywords
protein nitration, nitrotyrosine, nitric oxide, inflammation, oxidative stress, apoptosis, ATP production, metabolic proteins, asthma, sepsis, graft rejection, neurodegenerative diseases
MeSH terms
Animals, Humans, Interferon-gamma, Interleukin-1, Nitrates, Nitric Oxide, Tumor Cells, Cultured, Tumor Necrosis Factor-alpha, Electrophoresis, Gel, Two-Dimensional, Gene Expression, Rats, Sprague-Dawley, Nitric Oxide Synthase, Proteome, Rats, Nitric Oxide Synthase Type II

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