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Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissues.
Open accessAbstract
Monoclonal antibody MRK16 was used to determine the location of P-glycoprotein, the product of the multidrug-resistance gene (MDR1), in normal human tissues. The protein was found to be concentrated in a small number of specific sites. Most tissues examined revealed very little P-glycoprotein. However, certain cell types in liver, pancreas, kidney, colon, and jejunum showed specific localization of P-glycoprotein. In liver, P-glycoprotein was found exclusively on the biliary canalicular front of hepatocytes and on the apical surface of epithelial cells in small biliary ductules. In pancreas, P-glycoprotein was found on the apical surface of the epithelial cells of small ductules but not larger pancreatic ducts. In kidney, P-glycoprotein was found concentrated on the apical surface of epithelial cells of the proximal tubules. Colon and jejunum both showed high levels of P-glycoprotein on the apical surfaces of superficial columnar epithelial cells. Adrenal gland showed high levels of P-glycoprotein diffusely distributed on the surface of cells in both the cortex and medulla. These results suggest that the protein has a role in the normal secretion of metabolites and certain anti-cancer drugs into bile, urine, and directly into the lumen of the gastrointestinal tract.
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Metadata
- Authors
- Thiebaut, F., Tsuruo, T., Hamada, H., Gottesman, M. M., Pastan, I., & Willingham, M. C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1987-11
- Volume
- 84
- Issue
- 21
- Pages
- 7735-7738
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1964470226
- Cited by
- 2,777
- References
- 0
- FWCI
- 16.60
- Type
- journal article
- Language
- en
- Open access
- green
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