CoChat
Sign Up
Cited by 167Cell Adhesion Molecules ResearchVitamin C and Antioxidants ResearchAntioxidant Activity and Oxidative Stress

Vitamin C prevents cigarette smoke-induced leukocyte aggregation and adhesion to endothelium in vivo.

Open access
Lehr, H A, Frei, B, Arfors, K E·1994·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.91.16.7688PMC PMC44467
Open this paper in CoChat — free account

Abstract

A common feature of cigarette-smoke (CS)-associated diseases such as atherosclerosis and pulmonary emphysema is the activation, aggregation, and adhesion of leukocytes to micro- and macrovascular endothelium. A previous study, using a skinfold chamber model for intravital fluorescence microscopy in awake hamsters, has shown that exposure of hamsters to the smoke generated by one research cigarette elicits the adhesion of fluorescently labeled leukocytes to the endothelium of arterioles and small venules. By the combined use of intravital microscopy and scanning electron microscopy, we now demonstrate in the same animal model that (i) CS-induced leukocyte adhesion is not confined to the microcirculation, but that leukocytes also adhere singly and in clusters to the aortic endothelium; (ii) CS induces the formation in the bloodstream of aggregates between leukocytes and platelets; and (iii) CS-induced leukocyte adhesion to micro- and macrovascular endothelium and leukocyte-platelet aggregate formation are almost entirely prevented by dietary or intravenous pretreatment with the water-soluble antioxidant vitamin C (venules, 21.4 +/- 11.0 vs. 149.6 +/- 38.7 leukocytes per mm2, P < 0.01; arterioles, 8.5 +/- 4.2 vs. 54.3 +/- 21.6 leukocytes per mm2, P < 0.01; aortas, 0.8 +/- 0.4 vs. 12.4 +/- 5.6 leukocytes per mm2, P < 0.01; means +/- SD of n = 7 animals, 15 min after CS exposure). No inhibitory effect was observed by pretreatment of the animals with the lipid-soluble antioxidants vitamin E or probucol. The protective effects of vitamin C on CS-induced leukocyte adhesion and aggregation were seen at vitamin C plasma levels (55.6 +/- 22.2 microM, n = 7) that can easily be reached in humans by dietary means or supplementation, suggesting that vitamin C effectively contributes to protection from CS-associated cardiovascular and pulmonary diseases in humans.

Integrity signals

Registry

Crossref · 10.1073/pnas.91.16.7688

Cites

42 of 42 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

The text we found for this paper looks like another document, so its claims and self-check weren’t run. The registry and citation checks still apply.

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The text we found for this paper looks like another document, so the self-check wasn’t run.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Lehr, H. A., Frei, B., & Arfors, K. E.
Affiliations
Institut für Chirurgische Forschung (DE)
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
1994-08-02
Volume
91
Issue
16
Pages
7688-7692
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.91.16.7688
PMC
PMC44467
OpenAlex
W2052075519
Cited by
167
References
42
Citations by year
2026: 1 · 2025: 1 · 2024: 5 · 2023: 2 · 2022: 1 · 2020: 5 · 2019: 2 · 2018: 4 · 2017: 1 · 2016: 1 · 2015: 3 · 2014: 7 · 2013: 3 · 2012: 3
FWCI
3.88
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (7r29hl049954-05)
  • National Heart, Lung, and Blood Institute (HL49954)
Topics
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Health Sciences › Nursing › Nutrition and Dietetics › Vitamin C and Antioxidants Research
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
Keywords
vitamin C, cigarette smoke, leukocyte adhesion, leukocyte aggregation, platelet aggregation, endothelium, atherosclerosis, emphysema, antioxidants, vitamin E, probucol, intravital microscopy, hamster model, cardiovascular disease, lung disease
MeSH terms
Animals, Aorta, Ascorbic Acid, Cell Adhesion, Cell Aggregation, Endothelium, Vascular, Cricetinae, Leukocytes, Mesocricetus, Microcirculation, Microscopy, Electron, Scanning, Microscopy, Fluorescence, Probucol, Staining and Labeling, Superoxides, Vitamin E, Smoke Inhalation Injury, Reactive Oxygen Species

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review