Disruption of hippocampal development in vivo by CR-50 mAb against Reelin
Open accessAbstract
We previously generated a monoclonal alloantibody, CR-50, by immunizing reeler mutant mice with homogenates of normal embryonic brains. This antibody recently was shown to recognize a Reelin protein, which is coded by the recently identified candidate gene for the reeler mutation. However, it is still unclear whether Reelin, especially the CR-50 epitope region, is indeed responsible for the reeler phenotype in vivo . Here we show that Reelin is localized on Cajal–Retzius neurons in the hippocampus and that intraventricular injection of CR-50 at the embryonic stage disrupts the organized development of the hippocampus in vivo , converting it to a reeler pattern. Labeling experiments with 5-bromodeoxyuridine demonstrated that the labeled cells in the stratum pyramidale of the CR-50-treated mice were distributed in a pattern similar to that of reeler . Thus, Cajal–Retzius neurons play a crucial function in hippocampus development, and the CR-50 epitope on Reelin plays a central role in this function.
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CR-50 antibody has neutralizing activity of Reelin both in vitro and in vivo.
“The CR-50 antibody has neutralizing activity of Reelin both in vitro and in vivo (13, 19–21).”
From Reelin molecules assemble together to form a large protein complex, which is inhibited by the function-blocking CR-50 antibody (2000) · cites this paper as [21]
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Self-citation
- Authors
- Nakajima, K., Mikoshiba, K., Miyata, T., Kudo, C., & Ogawa, M.
- Affiliations
- RIKEN (JP)
- University of Kochi (JP)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1997-07-22
- Volume
- 94
- Issue
- 15
- Pages
- 8196-8201
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC21580
- OpenAlex
- W2018445004
- Cited by
- 171
- References
- 41
- Citations by year
- 2025: 4 · 2024: 1 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 3 · 2019: 4 · 2018: 2 · 2017: 2 · 2016: 8 · 2015: 6 · 2014: 3 · 2013: 2 · 2012: 8
- FWCI
- 3.79
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- reelin, hippocampal development, Cajal-Retzius neurons, antibody epitopes, intraventricular injection, embryonic brain, stratum pyramidale, BrdU labeling
- MeSH terms
- Reelin Protein, Animals, Antibodies, Monoclonal, Epitopes, Female, Hippocampus, Immunohistochemistry, Mice, Neurologic Mutants, Nerve Tissue Proteins, Phenotype, Pregnancy, Serine Endopeptidases, Cell Adhesion Molecules, Neuronal, Extracellular Matrix Proteins, Mice
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