The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Live Imaging Reveals Differing Roles of Macrophages and Neutrophils during Zebrafish Tail Fin Regeneration
Open accessAbstract
Macrophages and neutrophils are the pivotal immune phagocytes that enter the wound after tissue injury to remove the cell debris and invaded microorganisms, which presumably facilitate the regrowth of injured tissues. Taking advantage of the regeneration abilities of zebrafish and the newly generated leukocyte-specific zebrafish lines with labeling of both leukocyte lineages, we assessed the behaviors and functions of neutrophils and macrophages during tail fin regeneration. Live imaging showed that within 6 hours post amputation, the inflammatory stage, neutrophils were the primary cells scavenging apoptotic bodies and small cell debris, although they had limited phagocytic capacity and quickly underwent apoptosis. From 6 hours post amputation on, the resolution and regeneration stage, macrophages became the dominant scavengers, efficiently resolving inflammation and facilitating tissue remodeling and regrowth. Ablation of macrophages but not neutrophils severely impaired the inflammatory resolution and tissue regeneration, resulting in the formation of large vacuoles in the regenerated fins. In contrast, removal of neutrophils slightly accelerates the regrowth of injured fin. Our study documents the differing behaviors and functions of macrophages and neutrophils during tissue regeneration.
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“The zebrafish larval caudal fin regeneration model is a well-established system for studying the roles of the immune system in organ injury and regeneration [40]”
Citations
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ResultCoronins: the return of the crown
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MethodResultInfluentialHematopoietic gene expression profile in zebrafish kidney marrow
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“The full recovery of the wound normally requires only 4–5 days and recapitulates the process of tail fin regeneration in adult fish (10).”
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“Unlike other family members, Coronin 1a RNA is specifically synthesized by leukocytes (20).”
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Duffin, R., Leitch, A. E., Fox, S., Haslett, C., & Rossi, A. G. (2010) · Immunological Reviews · doi:10.1111/j.1600-065x.2010.00922.x · Cited by 126
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Gene expression and functional analysis of zebrafish larval fin fold regeneration
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“The epimorphic regeneration of tail fin in larva fish is one of thewell established tissue regeneration assays (10, 13).”
BackgroundCoronin-1 Function Is Required for Phagosome Formation
Yan, M., Collins, R. F., Grinstein, S., & Trimble, W. S. (2005) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-11-0989 · Cited by 92 · cited 1×
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“To study the real-time actions of macrophages and neutrophils in tissue regeneration, thewell established tail fin regeneration assay (14, 15, 38, 39) was employed in 72 hpf Tg(coro1a:eGFP;lyz:Dsred) embryos, making a lesion 251.”
MethodDistinct Functions for Different scl Isoforms in Zebrafish Primitive and Definitive Hematopoiesis
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“Fluorescence-activated Cell Sorting (FACS) and Giemsa Staining—The FACS analysis to isolate GFP-positive cells and Giemsa staining were performed as described previously (29, 30).”
MethodRelevance of granulocyte apoptosis to resolution of inflammation at the respiratory mucosa
Leitch, A. E., Duffin, R., Haslett, C., & Rossi, A. G. (2008) · Mucosal Immunology · doi:10.1038/mi.2008.31 · Cited by 76
Coronin Function Is Required for Chemotaxis and Phagocytosis in Human Neutrophils
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BackgroundChapter 19 Zebrafish Informatics and the ZFIN Database
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MethodJin, H., Xu, J., Qian, F., Du, L., Tan, C. Y., Lin, Z., Peng, J., & Wen, Z. (2005) · Developmental Dynamics · doi:10.1002/dvdy.20613 · Cited by 32 · cited 1×
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“Fluorescence-activated Cell Sorting (FACS) and Giemsa Staining—The FACS analysis to isolate GFP-positive cells and Giemsa staining were performed as described previously (29, 30).”
Method
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Neutrophil migration in infection and wound repair: going forward in reverse
de Oliveira, S., Rosowski, E. E., & Huttenlocher, A. (2016) · Nature Reviews Immunology · doi:10.1038/nri.2016.49 · Cited by 1,058
Preparing the ground for tissue regeneration: from mechanism to therapy
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Reciprocal Regulation between Resting Microglial Dynamics and Neuronal Activity In Vivo
Li, Y., Du, X.-F., Liu, C., Wen, Z., & Du, J. (2012) · Developmental Cell · doi:10.1016/j.devcel.2012.10.027 · Cited by 590
“To examine this hypothesis, we applied two-photon laser pulse (blue lightning symbols in Figure 6D) to induce local neuronal injury in intact optic tectum of Tg(Coronin1a:DsRed) larvae (Li et al., 2012), in which microglia express DsRed.”
MethodApoptotic cell: linkage of inflammation and wound healing
Wu, Y.-S., & Chen, S.-N. (2014) · Frontiers in Pharmacology · doi:10.3389/fphar.2014.00001 · Cited by 500
Cxcl8 (IL-8) Mediates Neutrophil Recruitment and Behavior in the Zebrafish Inflammatory Response
de Oliveira, S., Reyes‐Aldasoro, C. C., Candel, S., Renshaw, S. A., Mulero, V., & Calado, Â. (2013) · The Journal of Immunology · doi:10.4049/jimmunol.1203266 · Cited by 385
“Although the mechanisms involved in this process deserve further investigation, our results are in agreement with a recent study that reported the absence of neutrophils to contribute to a faster and more successful repair of the tail fin (58).”
Result
Metadata
- Authors
- Li, L., Yan, B., Shi, Y.-Q., Zhang, W.-Q., & Wen, Z.-L.
- Affiliations
- Southwest University (CN)
- Hong Kong University of Science and Technology (HK)
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2012-07
- Volume
- 287
- Issue
- 30
- Pages
- 25353-25360
- ISSN
- 0021-9258 (ISSN-L)
- PMC
- PMC3408142
- OpenAlex
- W1980758898
- Cited by
- 353
- References
- 49
- Citations by year
- 2026: 26 · 2025: 24 · 2024: 23 · 2023: 21 · 2022: 41 · 2021: 34 · 2020: 22 · 2019: 36 · 2018: 19 · 2017: 24 · 2016: 23 · 2015: 19 · 2014: 25 · 2013: 13 · 2012: 3
- FWCI
- 9.09
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Rare & Special e-Zone (The Hong Kong University of Science and Technology) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
- Health Sciences › Medicine › Surgery › Wound Healing and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Planarian Biology and Electrostimulation
- Keywords
- macrophages, neutrophils, tail fin regeneration, zebrafish, wound healing, phagocytosis, inflammation resolution, tissue remodeling, macrophage depletion, live imaging
- MeSH terms
- Animals, Macrophages, Neutrophils, Regeneration, Tail, Zebrafish, Animal Fins
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