Cited by 33Neuroscience and Neuropharmacology ResearchProteoglycans and glycosaminoglycans researchAmino Acid Enzymes and Metabolism

Chondroitin Sulfate Proteoglycan Tenascin-R Regulates Glutamate Uptake by Adult Brain Astrocytes

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Okuda, Hiroaki, Tatsumi, Kouko, Morita, Shoko, Shibukawa, Yukinao, Korekane, Hiroaki, Horii-Hayashi, Noriko, Wada, Yoshinao, Taniguchi, Naoyuki +1 more·2014·Journal of Biological Chemistry

Abstract

In our previous study, the CS-56 antibody, which recognizes a chondroitin sulfate moiety, labeled a subset of adult brain astrocytes, yielding a patchy extracellular matrix pattern. To explore the molecular nature of CS-56-labeled glycoproteins, we purified glycoproteins of the adult mouse cerebral cortex using a combination of anion-exchange, charge-transfer, and size-exclusion chromatographies. One of the purified proteins was identified as tenascin-R (TNR) by mass spectrometric analysis. When we compared TNR mRNA expression patterns with the distribution patterns of CS-56-positive cells, TNR mRNA was detected in CS-56-positive astrocytes. To examine the functions of TNR in astrocytes, we first confirmed that cultured astrocytes also expressed TNR protein. TNR knockdown by siRNA expression significantly reduced glutamate uptake in cultured astrocytes. Furthermore, expression of mRNA and protein of excitatory amino acid transporter 1 (GLAST), which is a major component of astrocytic glutamate transporters, was reduced by TNR knockdown. Our results suggest that TNR is expressed in a subset of astrocytes and contributes to glutamate homeostasis by regulating astrocytic GLAST expression.

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Authors
Okuda, H., Tatsumi, K., Morita, S., Shibukawa, Y., Korekane, H., Horii-Hayashi, N., Wada, Y., Taniguchi, N., & Wanaka, A.
Affiliations
  • Nara Medical University (JP)
  • Osaka Women's and Children's Hospital (JP)
  • RIKEN (JP)
Corresponding author
Okuda, Hiroaki
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2014-01
Volume
289
Issue
5
Pages
2620-2631
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2030236179
Cited by
33
References
59
Citations by year
2026: 2 · 2025: 3 · 2024: 2 · 2023: 3 · 2022: 5 · 2021: 6 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 1 · 2015: 4
FWCI
0.82
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Japan Society for the Promotion of Science (23500416, 25870634, 23390081, 24592141, 24591618, 26440051, 24593106)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
Keywords
tenascin-R, chondroitin sulfate proteoglycans, astrocytes, glutamate uptake, GLAST, glutamate homeostasis, extracellular matrix, cerebral cortex, siRNA knockdown
MeSH terms
Age Factors, Animals, Animals, Newborn, Astrocytes, Cells, Cultured, Cerebral Cortex, Chondroitin Sulfates, Glycoproteins, Homeostasis, Male, Mice, Inbred C57BL, RNA, Messenger, Glutamic Acid, Tenascin, Excitatory Amino Acid Transporter 1, RNA, Small Interfering, Mice

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