Chondroitin Sulfate Proteoglycan Tenascin-R Regulates Glutamate Uptake by Adult Brain Astrocytes
Open accessAbstract
In our previous study, the CS-56 antibody, which recognizes a chondroitin sulfate moiety, labeled a subset of adult brain astrocytes, yielding a patchy extracellular matrix pattern. To explore the molecular nature of CS-56-labeled glycoproteins, we purified glycoproteins of the adult mouse cerebral cortex using a combination of anion-exchange, charge-transfer, and size-exclusion chromatographies. One of the purified proteins was identified as tenascin-R (TNR) by mass spectrometric analysis. When we compared TNR mRNA expression patterns with the distribution patterns of CS-56-positive cells, TNR mRNA was detected in CS-56-positive astrocytes. To examine the functions of TNR in astrocytes, we first confirmed that cultured astrocytes also expressed TNR protein. TNR knockdown by siRNA expression significantly reduced glutamate uptake in cultured astrocytes. Furthermore, expression of mRNA and protein of excitatory amino acid transporter 1 (GLAST), which is a major component of astrocytic glutamate transporters, was reduced by TNR knockdown. Our results suggest that TNR is expressed in a subset of astrocytes and contributes to glutamate homeostasis by regulating astrocytic GLAST expression.
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These clusters were shown in rodents to surround a small number of neurons and regulate glutamatergic transmission.
“These clusters were shown in rodents to surround a small number of neurons and regulate glutamatergic transmission.70, 100, 101”
From Aggrecan and chondroitin-6-sulfate abnormalities in schizophrenia and bipolar disorder: a postmortem study on the amygdala (2015) · cites this paper as [101]
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- Authors
- Okuda, H., Tatsumi, K., Morita, S., Shibukawa, Y., Korekane, H., Horii-Hayashi, N., Wada, Y., Taniguchi, N., & Wanaka, A.
- Affiliations
- Nara Medical University (JP)
- Osaka Women's and Children's Hospital (JP)
- RIKEN (JP)
- Corresponding author
- Okuda, Hiroaki
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2014-01
- Volume
- 289
- Issue
- 5
- Pages
- 2620-2631
- ISSN
- 0021-9258 (ISSN-L)
- PMC
- PMC3908396
- OpenAlex
- W2030236179
- Cited by
- 33
- References
- 59
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 2 · 2023: 3 · 2022: 5 · 2021: 6 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 1 · 2015: 4
- FWCI
- 0.82
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (23500416, 25870634, 23390081, 24592141, 24591618, 26440051, 24593106)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Keywords
- tenascin-R, chondroitin sulfate proteoglycans, astrocytes, glutamate uptake, GLAST, glutamate homeostasis, extracellular matrix, cerebral cortex, siRNA knockdown
- MeSH terms
- Age Factors, Animals, Animals, Newborn, Astrocytes, Cells, Cultured, Cerebral Cortex, Chondroitin Sulfates, Glycoproteins, Homeostasis, Male, Mice, Inbred C57BL, RNA, Messenger, Glutamic Acid, Tenascin, Excitatory Amino Acid Transporter 1, RNA, Small Interfering, Mice
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