Morphology of perineuronal nets in tenascin-R and parvalbumin single and double knockout mice
Abstract
Recently identified chondroitin sulphate proteoglycans in perineuronal nets include neurocan and phosphacan. However, the function and assembly of these components has yet to be resolved. In this study we show morphological alteration in Wisteria floribunda labelled nets around cortical interneurones both in tenascin-R knockout and tenascin-R/parvalbumin double knockout mice. This alteration reflects the loss of phosphacan and neurocan from cortical nets in mice deficient in tenascin-R. No effect on the membrane related cytoskeleton, as revealed by ankyrin(R), was observed in any of the mice. These results on mice lacking tenascin-R substantiate previously reported in vitro interactions between tenascin-R and phosphacan and neurocan.
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VVA staining was used as a reliable marker for visualizing Pvalb neurons, irrespective of reduced or absent PV expression.
“Since neither the morphology nor the distribution of PNNs were shown to be altered in PV+/- and PV-/- mice in comparison to PV+/+ mice [57, 69], VVA staining was used as reliable marker for visualizing the specific neuronal subpopulation of “PV-FSI” cells, defined as Pvalb neurons, irrespective of reduced or absent PV expression in PV+/- and PV-/- mice, respectively.”
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- Authors
- Haunsø, A., Ibrahim, M., Bartsch, U., Letiembre, M., Celio, M. R., & Menoud, P.-A.
- Affiliations
- University of Fribourg (CH)
- Universität Hamburg (DE)
- Corresponding author
- Menoud, Pierre-Alain
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 2000-05
- Volume
- 864
- Issue
- 1
- Pages
- 142-145
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W2023467917
- Cited by
- 61
- References
- 20
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 7 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 4 · 2016: 4 · 2015: 2 · 2013: 1 · 2012: 2
- FWCI
- 1.03
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- perineuronal nets, tenascin-R, parvalbumin, phosphacan, neurocan, chondroitin sulfate proteoglycans, cortical interneurons, ankyrin, membrane cytoskeleton, knockout mice
- MeSH terms
- Animals, Cerebral Cortex, Extracellular Matrix, Fluorescent Antibody Technique, Nerve Tissue Proteins, Neurons, Parvalbumins, Chondroitin Sulfate Proteoglycans, Mice, Knockout, Tenascin, Lectins, C-Type, Cell Size, Mice, Receptor-Like Protein Tyrosine Phosphatases, Class 5, Neurocan
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