Cited by 692Nitric Oxide and Endothelin EffectsRedox biology and oxidative stressAntioxidant Activity and Oxidative Stress

The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.

Interactions of Peroxynitrite, Tetrahydrobiopterin, Ascorbic Acid, and Thiols

Open access

Kuzkaya, Nermin, Weissmann, Norbert, Harrison, David G., Dikalov, Sergey·2003·Journal of Biological Chemistry

DOI 10.1074/jbc.m302227200

Open this paper in CoChat — free account Open PDF

Abstract

Tetrahydrobiopterin (BH4) serves as a critical co-factor for the endothelial nitric-oxide synthase (eNOS). A deficiency of BH4 results in eNOS uncoupling, which is associated with increased superoxide and decreased NO* production. BH4 has been suggested to be a target for oxidation by peroxynitrite (ONOO-), and ascorbate has been shown to preserve BH4 levels and enhance endothelial NO* production; however, the mechanisms underlying these processes remain poorly defined. To gain further insight into these interactions, the reaction of ONOO- with BH4 was studied using electron spin resonance and the spin probe 1-hydroxy-3-carboxy-2,2,5-tetramethyl-pyrrolidine. ONOO- reacted with BH4 6-10 times faster than with ascorbate or thiols. The immediate product of the reaction between ONOO- and BH4 was the trihydrobiopterin radical (BH3.), which was reduced back to BH4 by ascorbate, whereas thiols were not efficient in recycling of BH4. Uncoupling of eNOS caused by peroxynitrite was investigated in cultured bovine aortic endothelial cells (BAECs) by measuring superoxide and NO* using spin probe 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine and the NO*-spin trap iron-diethyldithiocarbamate. Bolus ONOO-, the ONOO- donor 3-morpholinosydnonimine, and an inhibitor of BH4 synthesis (2,4-diamino-6-hydroxypyrimidine) uncoupled eNOS, increasing superoxide and decreasing NO* production. Exogenous BH4 supplementation restored endothelial NO* production. Treatment of BAECs with both BH4 and ascorbate prior to ONOO- prevented uncoupling of eNOS by ONOO-. This study demonstrates that endothelial BH4 is a crucial target for oxidation by ONOO- and that the BH4 reaction rate constant exceeds those of thiols or ascorbate. We confirmed that ONOO- uncouples eNOS by oxidation of tetrahydrobiopterin and that ascorbate does not fully protect BH4 from oxidation but recycles BH3. radical back to BH4.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1074/jbc.m302227200

Cites

51 of 51 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Nitric oxide synthases: structure, function and inhibition
ALDERTON, W. K., COOPER, C. E., & KNOWLES, R. G. (2001) · Biochemical Journal · doi:10.1042/bj3570593 · Cited by 4,110
Peroxynitrite oxidation of sulfhydryls.
Radí, R., Beckman, J. S., Bush, K., & Freeman, B. Α. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)64313-7 · Cited by 2,492
Regulation of biosynthesis of nitric oxide.
Nathan, C., & Xie, Q. W. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)36703-0 · Cited by 1,669
Oxidation of tetrahydrobiopterin leads to uncoupling of endothelial cell nitric oxide synthase in hypertension
Landmesser, U., Dikalov, S., Price, S. R., McCann, L., Fukai, T., Holland, S. M., Mitch, W. E., & Harrison, D. G. (2003) · Journal of Clinical Investigation · doi:10.1172/jci200314172 · Cited by 1,660
Self-citation
Oxidation of tetrahydrobiopterin leads to uncoupling of endothelial cell nitric oxide synthase in hypertension
Landmesser, U., Dikalov, S., Price, S. R., McCann, L. A., Fukai, T., Holland, S. M., Mitch, W. E., & Harrison, D. G. (2003) · Journal of Clinical Investigation · doi:10.1172/jci14172 · Cited by 1,652
Self-citation
Superoxide generation by endothelial nitric oxide synthase: The influence of cofactors
Vásquez-Vivar, J., Kalyanaraman, B., Martásek, P., Hogg, N., Masters, B. S. S., Karoui, H., Tordo, P., & Pritchard, K. A. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.16.9220 · Cited by 1,484
Nitric oxide synthase structure and mechanism
Marletta, M. A. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)31375-9 · Cited by 1,338
Mammalian nitric oxide synthases
Stuehr, D. J. (1999) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/s0005-2728(99)00016-x · Cited by 996
Simulation of Multiple Isotropic Spin-Trap EPR Spectra
Duling, D. (1994) · Journal of Magnetic Resonance Series B · doi:10.1006/jmrb.1994.1062 · Cited by 940
Vitamin C improves endothelium-dependent vasodilation in patients with non-insulin-dependent diabetes mellitus.
Ting, H. H., Timimi, F. K., Boles, K., Creager, S. J., Ganz, P., & Creager, M. A. (1996) · Journal of Clinical Investigation · doi:10.1172/jci118394 · Cited by 869

Show all 50 references

Research works printed but not linked: 3

Gorren, 1998, Biochemistry (Mosc.), 63, 734
Dikalov, 1998, J. Pharmacol. Exp. Ther., 286, 938
Fink, 2002, Free Radic. Biol. Med., 33, 366

Sorted by citations

Nitric Oxide and Peroxynitrite in Health and Disease
Pacher, P., Beckman, J. S., & Liaudet, L. (2007) · Physiological Reviews · doi:10.1152/physrev.00029.2006 · Cited by 6,390
Peroxynitrite: biochemistry, pathophysiology and development of therapeutics
Szabó, C., Ischiropoulos, H., & Radi, R. (2007) · Nature Reviews Drug Discovery · doi:10.1038/nrd2222 · Cited by 2,134
Endothelial Nitric Oxide Synthase in Vascular Disease: From Marvel to Menace
Förstermann, U., & Münzel, T. (2006) · Circulation · doi:10.1161/circulationaha.105.602532 · Cited by 1,957
Vitamins C and E: Beneficial effects from a mechanistic perspective
Traber, M. G., & Stevens, J. F. (2011) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2011.05.017 · Cited by 1,076
Copper, oxidative stress, and human health
Uriu‐Adams, J. Y., & Keen, C. L. (2005) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2005.07.015 · Cited by 995
Nitric oxide signaling in health and disease
Lundberg, J. O., & Weitzberg, E. (2022) · Cell · doi:10.1016/j.cell.2022.06.010 · Cited by 879
Vascular nitric oxide: Beyond eNOS
Zhao, Y., Vanhoutte, P. M., & Leung, S. W. S. (2015) · Journal of Pharmacological Sciences · doi:10.1016/j.jphs.2015.09.002 · Cited by 875
Endothelial dysfunction: a multifaceted disorder (The Wiggers Award Lecture)
Félétou, M., & Vanhoutte, P. M. (2006) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00292.2006 · Cited by 818
Endothelial Function and Oxidative Stress in Cardiovascular Diseases
Higashi, Y., Noma, K., Yoshizumi, M., & Kihara, Y. (2009) · Circulation Journal · doi:10.1253/circj.cj-08-1102 · Cited by 816
Cross talk between mitochondria and NADPH oxidases
Dikalov, S. (2011) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2011.06.033 · Cited by 783
Self-citation
Nitric oxide and oxidative stress in vascular disease
Förstermann, U. (2010) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/s00424-010-0808-2 · Cited by 796
Role of Oxidative Stress in Cardiac Hypertrophy and Remodeling
Takimoto, E., & Kass, D. A. (2006) · Hypertension · doi:10.1161/01.hyp.0000254415.31362.a7 · Cited by 784
Endothelial Dysfunction and Diabetes: Effects on Angiogenesis, Vascular Remodeling, and Wound Healing
Kolluru, G. K., Bir, S. C., & Kevil, C. G. (2012) · International Journal of Vascular Medicine · doi:10.1155/2012/918267 · Cited by 752
The Right Ventricle Under Pressure
Bogaard, H. J., Abe, K., Noordegraaf, A. V., & Voelkel, N. F. (2009) · CHEST Journal · doi:10.1378/chest.08-0492 · Cited by 692
Nitric Oxide and Endothelial Dysfunction
Cyr, A. R., Huckaby, L. V., Shiva, S. S., & Zuckerbraun, B. S. (2020) · Critical Care Clinics · doi:10.1016/j.ccc.2019.12.009 · Cited by 712
Biochemistry of Peroxynitrite and Protein Tyrosine Nitration
Ferrer‐Sueta, G., Campolo, N., Trujillo, M., Bartesaghi, S., Carballal, S., Romero, N., Álvarez, B., & Radí, R. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00568 · Cited by 653
Oxidative Stress in Preeclampsia and Placental Diseases
Aouache, R., Biquard, L., Vaiman, D., & Miralles, F. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19051496 · Cited by 645
Inflammation, Oxidative Stress, and Repair Capacity of the Vascular Endothelium in Obstructive Sleep Apnea
Jelić, S., Padeletti, M., Kawut, S. M., Higgins, C., Canfield, S. M., Onat, D., Colombo, P. C., Basner, R. C., Factor, P. H., & LeJemtel, T. H. (2008) · Circulation · doi:10.1161/circulationaha.107.741512 · Cited by 633
The role of nitric oxide in cardiovascular diseases
NASEEM, K. (2005) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2004.09.003 · Cited by 557
In vitro assessments of nanomaterial toxicity
Jones, C. F., & Grainger, D. W. (2009) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2009.03.005 · Cited by 558
Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation
Tejero, J., Shiva, S., & Gladwin, M. T. (2019) · Physiological Reviews · doi:10.1152/physrev.00036.2017 · Cited by 541
Measurement of Reactive Oxygen Species in Cardiovascular Studies
Dikalov, S., Griendling, K. K., & Harrison, D. G. (2007) · Hypertension · doi:10.1161/01.hyp.0000258594.87211.6b · Cited by 532
Self-citation
Regulation of Endothelial Nitric Oxide Synthase by Tetrahydrobiopterin in Vascular Disease
Alp, N. J., & Channon, K. M. (2003) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000110785.96039.f6 · Cited by 536
Endothelial dysfunction : the early predictor of atherosclerosis
Mudau, M., Genis, A., Lochner, A., & Strijdom, H. (2012) · Cardiovascular Journal of Africa · doi:10.5830/cvja-2011-068 · Cited by 521
Oxidative stress and endothelial dysfunction in hypertension
Schulz, E., Gori, T., & Münzel, T. F. (2011) · Hypertension Research · doi:10.1038/hr.2011.39 · Cited by 516
Is oxidative stress a therapeutic target in cardiovascular disease?
Münzel, T., Gori, T., Bruno, R. M., & Taddei, S. (2010) · European Heart Journal · doi:10.1093/eurheartj/ehq396 · Cited by 470
Nitric oxide synthase inhibition and oxidative stress in cardiovascular diseases: Possible therapeutic targets?
Rochette, L., Lorin, J., Zeller, M., Guilland, J.-C., Lorgis, L., Cottin, Y., & Vergely, C. (2013) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2013.07.004 · Cited by 456
Dysfunction of Endothelial Nitric Oxide Synthase and Atherosclerosis
Kawashima, S., & Yokoyama, M. (2004) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000125114.88079.96 · Cited by 455
Oxidative Stress and Hypertension
Harrison, D. G., & Góngora, M. C. (2009) · Medical Clinics of North America · doi:10.1016/j.mcna.2009.02.015 · Cited by 455
Self-citation
Oxidant stress from nitric oxide synthase–3 uncoupling stimulates cardiac pathologic remodeling from chronic pressure load
Takimoto, E., Champion, H. C., Li, M., Ren, S. V., Rodríguez, E. R., Tavazzi, B., Lazzarino, G., Paolocci, N., Gabrielson, K. L., Wang, Y., et al. (2005) · Journal of Clinical Investigation · doi:10.1172/jci21968 · Cited by 446
The role of oxidative stress in the pathophysiology of hypertension
Rodrigo, R., González, J., & Paoletto, F. (2011) · Hypertension Research · doi:10.1038/hr.2010.264 · Cited by 436
Nitric oxide, tetrahydrobiopterin, oxidative stress, and endothelial dysfunction in hypertension
Schulz, E., Jansen, T., Wenzel, P., Daiber, A., & Münzel, T. (2008) · Antioxidants & Redox Signaling · doi:10.1089/ars.2007.1989 · Cited by 430
Oxidative Stress-Induced Endothelial Dysfunction in Cardiovascular Diseases
Shaito, A. A., Aramouni, K., Assaf, R., Parenti, A., Orekhov, A. N., EL‐Yazbi, A. F., Pintus, G., & Eid, A. H. (2022) · Frontiers in Bioscience-Landmark · doi:10.31083/j.fbl2703105 · Cited by 449
Explaining the Phenomenon of Nitrate Tolerance
Münzel, T., Daiber, A., & Mülsch, A. (2005) · Circulation Research · doi:10.1161/01.res.0000184694.03262.6d · Cited by 424
Cyclic GMP signaling in cardiovascular pathophysiology and therapeutics
Tsai, E. J., & Kass, D. A. (2009) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2009.02.009 · Cited by 437
Endothelial Nitric Oxide Synthase Uncoupling Impairs Endothelial Progenitor Cell Mobilization and Function in Diabetes
Thum, T., Fraccarollo, D., Schultheiß, M., Froese, S., Galuppo, P., Widder, J. D., Tsikas, D., Ertl, G., & Bauersachs, J. K. (2007) · Diabetes · doi:10.2337/db06-0699 · Cited by 416
Pathophysiology, diagnosis and prognostic implications of endothelial dysfunction
Münzel, T. F., Sinning, C., Post, F., Warnholtz, A., & Schulz, E. (2008) · Annals of Medicine · doi:10.1080/07853890701854702 · Cited by 413
Endothelium-derived microparticles impair endothelial function in vitro
Brodsky, S. V., Zhang, F., Nasjletti, A., & Goligorsky, M. S. (2004) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.01172.2003 · Cited by 379
The Role of Oxidative Stress in Cardiac Disease: From Physiological Response to Injury Factor
D’Oria, R., Schipani, R., Leonardini, A., Natalicchio, A., Perrini, S., Cignarelli, A., Laviola, L., & Giorgino, F. (2020) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2020/5732956 · Cited by 399
Atrial Fibrillation Increases Production of Superoxide by the Left Atrium and Left Atrial Appendage
Dudley, S. C., Hoch, N. E., McCann, L. A., Honeycutt, C., Diamandopoulos, L., Fukai, T., Harrison, D. G., Dikalov, S., & Langberg, J. J. (2005) · Circulation · doi:10.1161/circulationaha.105.538108 · Cited by 375
Self-citation
5-Methyltetrahydrofolate Rapidly Improves Endothelial Function and Decreases Superoxide Production in Human Vessels: Effects on Vascular Tetrahydrobiopterin Availability and Endothelial Nitric Oxide Synthase Coupling
Antoniades, C., Shirodaria, C., Warrick, N., Cai, S., de Bono, J., Lee, J., Leeson, P., Neubauer, S., Ratnatunga, C., Pillai, R., et al. (2006) · Circulation · doi:10.1161/circulationaha.106.612325 · Cited by 365
Mechanisms of homocysteine‐induced atherothrombosis
Lentz, S. R. (2005) · Journal of Thrombosis and Haemostasis · doi:10.1111/j.1538-7836.2005.01364.x · Cited by 375
NO/redox disequilibrium in the failing heart and cardiovascular system
Hare, J. M., & Stamler, J. S. (2005) · Journal of Clinical Investigation · doi:10.1172/jci200524459 · Cited by 361
Role of Vitamin C in the Function of the Vascular Endothelium
May, J. M., & Harrison, F. E. (2013) · Antioxidants & Redox Signaling · doi:10.1089/ars.2013.5205 · Cited by 358
Dysregulation of Antioxidant Mechanisms Contributes to Increased Oxidative Stress in Calcific Aortic Valvular Stenosis in Humans
Miller, J. D., Chu, Y., Brooks, R. M., Richenbacher, W. E., Peña-Silva, R., & Heistad, D. D. (2008) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2008.05.043 · Cited by 357
NAD(P)H oxidase and uncoupled nitric oxide synthase are major sources of glomerular superoxide in rats with experimental diabetic nephropathy
Satoh, M., Fujimoto, S., Haruna, Y., Arakawa, S., Horike, H., Komai, N., Sasaki, T., Tsujioka, K., Makino, H., & Kashihara, N. (2005) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.00221.2004 · Cited by 355
Obesity and type 2 diabetes
Maggio, C. A., & Pi-Sunyer, X. (2003) · Endocrinology and Metabolism Clinics of North America · doi:10.1016/s0889-8529(03)00071-9 · Cited by 364
Inducible NOS Inhibition Reverses Tobacco-Smoke-Induced Emphysema and Pulmonary Hypertension in Mice
Seimetz, M., Parajuli, N., Pichl, A., Veit, F., Kwapiszewska, G., Weisel, F. C., Milger, K., Egemnazarov, B., Turowska, A. M., Fuchs, B., et al. (2011) · Cell · doi:10.1016/j.cell.2011.08.035 · Cited by 352
Redox signaling (cross-talk) from and to mitochondria involves mitochondrial pores and reactive oxygen species
Daiber, A. (2010) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/j.bbabio.2010.01.032 · Cited by 347
Reactive oxygen species in hypertension*1An update
Lassegue, B. P. (2004) · American Journal of Hypertension · doi:10.1016/j.amjhyper.2004.02.004 · Cited by 351
The aftermath of the interplay between the endoplasmic reticulum stress response and redox signaling
Bhattarai, K. R., Riaz, T. A., Kim, H., & Chae, H. (2021) · Experimental & Molecular Medicine · doi:10.1038/s12276-021-00560-8 · Cited by 356
The basics about nitric oxide
Bruckdorfer, R. K. (2005) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2004.09.002 · Cited by 333
eNOS Uncoupling in Cardiovascular Diseases - the Role of Oxidative Stress and Inflammation
Karbach, S., Wenzel, P., Waisman, A., Munzel, T., & Daiber, A. (2014) · Current Pharmaceutical Design · doi:10.2174/13816128113196660748 · Cited by 307
Reactive oxygen species and ischemic cerebrovascular disease
Olmez, I., & Özyurt, H. (2011) · Neurochemistry International · doi:10.1016/j.neuint.2011.11.009 · Cited by 307
Cross-Talk between NADPH Oxidase and Mitochondria: Role in ROS Signaling and Angiogenesis
Fukai, T., & Ushio‐Fukai, M. (2020) · Cells · doi:10.3390/cells9081849 · Cited by 313
New Therapeutic Implications of Endothelial Nitric Oxide Synthase (eNOS) Function/Dysfunction in Cardiovascular Disease
Daiber, A., Xia, N., Steven, S., Oelze, M. W., Hanf, A., Kröller‐Schön, S., Münzel, T. F., & Li, H. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20010187 · Cited by 322
Therapeutic effect of enhancing endothelial nitric oxide synthase (eNOS) expression and preventing eNOS uncoupling
Förstermann, U., & Li, H. (2010) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.2010.01196.x · Cited by 306
Oscillatory Shear Stress Stimulates Endothelial Production of O2- from p47 -dependent NAD(P)H Oxidases, Leading to Monocyte Adhesion
Hwang, J., Saha, A., Boo, Y. C., Sorescu, G. P., McNally, J. S., Holland, S. M., Dikalov, S., Giddens, D. P., Griendling, K. K., Harrison, D. G., et al. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m305150200 · Cited by 299
Self-citation
Oxidative stress and metabolic pathologies: from an adipocentric point of view
Le Lay, S., Simard, G., Martinez, M. C., & Andriantsitohaina, R. (2014) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2014/908539 · Cited by 291
The Adverse Effects of Environmental Noise Exposure on Oxidative Stress and Cardiovascular Risk
Münzel, T. F., Sørensen, M., Schmidt, F. P., Schmidt, E. R., Steven, S., Kröller‐Schön, S., & Daiber, A. (2018) · Antioxidants and Redox Signaling · doi:10.1089/ars.2017.7118 · Cited by 294
Effects of noise on vascular function, oxidative stress, and inflammation: mechanistic insight from studies in mice
Münzel, T. F., Daiber, A., Steven, S., Tran, L. P., Ullmann, E., Kossmann, S., Schmidt, F. P., Oelze, M. W., Xia, N., Li, H., et al. (2017) · European Heart Journal · doi:10.1093/eurheartj/ehx081 · Cited by 292
Identification of the biologically active liquid chemistry induced by a nonthermal atmospheric pressure plasma jet
Wende, K., Williams, P., Dalluge, J., Gaens, W. V., Aboubakr, H. A., Bischof, J. C., von Woedtke, T., Goyal, S. M., Weltmann, K., Bogaerts, A., et al. (2015) · Biointerphases · doi:10.1116/1.4919710 · Cited by 280
Crosstalk of mitochondria with NADPH oxidase via reactive oxygen and nitrogen species signalling and its role for vascular function
Daiber, A., Lisa, F. D., Oelze, M. W., Kröller‐Schön, S., Steven, S., Schulz, E., & Münzel, T. F. (2015) · British Journal of Pharmacology · doi:10.1111/bph.13403 · Cited by 274
Peroxynitrite and Vascular Endothelial Dysfunction in Diabetes Mellitus
Zou, M., Cohen, R. A., & Ullrich, V. (2004) · Endothelium · doi:10.1080/10623320490482619 · Cited by 270
Targeting eNOS for stroke protection
Endres, M., Laufs, U., Liao, J. K., & Moskowitz, M. A. (2004) · Trends in Neurosciences · doi:10.1016/j.tins.2004.03.009 · Cited by 272
Endothelial dysfunction and hypertension in aging
Higashi, Y., Kihara, Y., & Noma, K. (2012) · Hypertension Research · doi:10.1038/hr.2012.138 · Cited by 277
Detection of intracellular superoxide formation in endothelial cells and intact tissues using dihydroethidium and an HPLC-based assay
Fink, B., Laude, K. M., McCann, L. A., Doughan, A. K., Harrison, D. G., & Dikalov, S. (2004) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00028.2004 · Cited by 259
Self-citation
Mitochondrial Oxidative Stress, Mitochondrial DNA Damage and Their Role in Age-Related Vascular Dysfunction
Mikhed, Y., Daiber, A., & Steven, S. (2015) · International Journal of Molecular Sciences · doi:10.3390/ijms160715918 · Cited by 257
Redox Signaling, Vascular Function, and Hypertension
Lee, M. Y., & Griendling, K. K. (2008) · Antioxidants and Redox Signaling · doi:10.1089/ars.2007.1986 · Cited by 249
Hyperglycemia in Acute Ischemic Stroke: A Vascular Perspective
Martini, S. R., & Kent, T. A. (2006) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/sj.jcbfm.9600355 · Cited by 242
Mechanisms and consequences of endothelial nitric oxide synthase dysfunction in hypertension
Li, Q., Youn, J.-Y., & Cai, H. “. (2015) · Journal of Hypertension · doi:10.1097/hjh.0000000000000587 · Cited by 244
Vascular NADPH oxidases as drug targets for novel antioxidant strategies
Guzik, T. J., & Harrison, D. G. (2006) · Drug Discovery Today · doi:10.1016/j.drudis.2006.04.003 · Cited by 238
Self-citation
Tetrahydrobiopterin in Cardiovascular Health and Disease
Bendall, J. K., Douglas, G., McNeill, E., Channon, K. M., & Crabtree, M. J. (2014) · Antioxidants & Redox Signaling · doi:10.1089/ars.2013.5566 · Cited by 231
Evaluating Oxidative Stress in Human Cardiovascular Disease: Methodological Aspects and Considerations
Lee, R., Margaritis, M., Channon, K. M., & Antoniades, C. A. (2012) · Current Medicinal Chemistry · doi:10.2174/092986712800493057 · Cited by 233
Evidence of endothelial dysfunction in the development of Alzheimer's disease: Is Alzheimer's a vascular disorder?
Kelleher, R. J., & Soiza, R. L. (2013) · Europe PMC (PubMed Central) · Cited by 232
Interactions of peroxynitrite with uric acid in the presence of ascorbate and thiols: Implications for uncoupling endothelial nitric oxide synthase
Kuzkaya, N., Weißmann, N., Harrison, D. G., & Dikalov, S. (2005) · Biochemical Pharmacology · doi:10.1016/j.bcp.2005.05.009 · Cited by 225
Self-citation
Tackling endothelial dysfunction by modulating NOS uncoupling: new insights into its pathogenesis and therapeutic possibilities
Kietadisorn, R., Juni, R. P., & Moens, A. L. (2011) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00540.2011 · Cited by 221
Oxidative stress and endothelial dysfunction: Clinical evidence and therapeutic implications
Higashi, Y., Maruhashi, T., Noma, K., & Kihara, Y. (2013) · Trends in Cardiovascular Medicine · doi:10.1016/j.tcm.2013.12.001 · Cited by 222
Ratio of 5,6,7,8-tetrahydrobiopterin to 7,8-dihydrobiopterin in endothelial cells determines glucose-elicited changes in NO vs. superoxide production by eNOS
Crabtree, M. J., Smith, C. L., Lam, G., Goligorsky, M. S., & Gross, S. S. (2008) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00823.2007 · Cited by 213
Tobacco smoking induces cardiovascular mitochondrial oxidative stress, promotes endothelial dysfunction, and enhances hypertension
Dikalov, S., Itani, H. A., Richmond, B. W., Arslanbaeva, L., Vergeade, A., Rahman, S. M. J., Boutaud, O. G., Blackwell, T. S., Massion, P. P., Harrison, D. G., et al. (2019) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00595.2018 · Cited by 221
Self-citation
S-Nitrosylation: An Emerging Paradigm of Redox Signaling
Fernando, V., Zheng, X., Walia, Y., Sharma, V., Letson, J., & Furuta, S. (2019) · Antioxidants · doi:10.3390/antiox8090404 · Cited by 225
Stoichiometric Relationships Between Endothelial Tetrahydrobiopterin, Endothelial NO Synthase (eNOS) Activity, and eNOS Coupling in Vivo
Bendall, J. K., Alp, N. J., Warrick, N., Cai, S., Adlam, D., Rockett, K. A., Yokoyama, M., Kawashima, S., & Channon, K. M. (2005) · Circulation Research · doi:10.1161/01.res.0000187447.03525.72 · Cited by 209
Role of Extracellular Superoxide Dismutase in Hypertension
Gongora, M. C., Qin, Z., Laude, K., Kim, H. W., McCann, L., Folz, J. R., Dikalov, S., Fukai, T., & Harrison, D. G. (2006) · Hypertension · doi:10.1161/01.hyp.0000235682.47673.ab · Cited by 204
Self-citation
Quantitative Regulation of Intracellular Endothelial Nitric-oxide Synthase (eNOS) Coupling by Both Tetrahydrobiopterin-eNOS Stoichiometry and Biopterin Redox Status
Crabtree, M. J., Tatham, A. L., Al-Wakeel, Y., Warrick, N., Hale, A. B., Cai, S., Channon, K. M., & Alp, N. J. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m805403200 · Cited by 200
Mechanisms for the role of tetrahydrobiopterin in endothelial function and vascular disease
Schmidt, T. S., & Alp, N. J. (2007) · Clinical Science · doi:10.1042/cs20070108 · Cited by 195
Essential hypertension and oxidative stress: New insights
González, J. (2014) · World Journal of Cardiology · doi:10.4330/wjc.v6.i6.353 · Cited by 196
Tetrahydrobiopterin
Channon, K. M. (2004) · Trends in Cardiovascular Medicine · doi:10.1016/j.tcm.2004.10.003 · Cited by 196
Increased Endothelial Tetrahydrobiopterin Synthesis by Targeted Transgenic GTP-Cyclohydrolase I Overexpression Reduces Endothelial Dysfunction and Atherosclerosis in ApoE-Knockout Mice
Alp, N. J., McAteer, M. A., Khoo, J., Choudhury, R. P., & Channon, K. M. (2004) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/01.atv.0000115637.48689.77 · Cited by 194
The Role of Oxidative Stress in Hypertensive Disorders of Pregnancy (Preeclampsia, Gestational Hypertension) and Metabolic Disorder of Pregnancy (Gestational Diabetes Mellitus)
Phoswa, W. N., & Khaliq, O. P. (2021) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2021/5581570 · Cited by 218
Tetrahydrobiopterin and Cardiovascular Disease
Moens, A. L., & Kass, D. A. (2006) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000243924.00970.cb · Cited by 194
Myocardial ischemia results in tetrahydrobiopterin (BH 4 ) oxidation with impaired endothelial function ameliorated by BH 4
Dumitrescu, C., Biondi, R., Xia, Y., Cardounel, A. J., Druhan, L. J., Ambrosio, G., & Zweier, J. L. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0702986104 · Cited by 192
ROS-responsive microspheres for on demand antioxidant therapy in a model of diabetic peripheral arterial disease
Poole, K. M., Nelson, C. E., Joshi, R. V., Martin, J. R., Gupta, M. K., Haws, S. C., Kavanaugh, T. E., Skala, M. C., & Duvall, C. L. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2014.11.016 · Cited by 189
Recent Advances in Understanding Endothelial Dysfunction in Atherosclerosis
Yang, Z., & Ming, X. (2006) · Clinical Medicine & Research · doi:10.3121/cmr.4.1.53 · Cited by 191
Nitric Oxide: A Guardian for Vascular Grafts?
de Mel, A., Murad, F., & Seifalian, A. M. (2011) · Chemical Reviews · doi:10.1021/cr200008n · Cited by 190
Nitric oxide and nitric oxide synthase isoforms in the normal, hypertrophic, and failing heart
Umar, S., & van der Laarse, A. (2010) · Molecular and Cellular Biochemistry · doi:10.1007/s11010-009-0219-x · Cited by 182
Upregulation of nox1 in vascular smooth muscle leads to impaired endothelium-dependent relaxation via enos uncoupling
Dikalova, A. E., Góngora, M. C., Harrison, D. G., Lambeth, J. D., Dikalov, S., & Griendling, K. K. (2010) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00242.2010 · Cited by 179
Self-citation
Proatherosclerotic Mechanisms Involving Protein Kinase C in Diabetes and Insulin Resistance
Rask‐Madsen, C., & King, G. L. (2005) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000155325.41507.e0 · Cited by 176
EPR detection of cellular and mitochondrial superoxide using cyclic hydroxylamines
Dikalov, S., Kirilyuk, I. A., Voinov, M. A., & Григорьев, И. А. (2010) · Free Radical Research · doi:10.3109/10715762.2010.540242 · Cited by 177
Self-citation
Mining literature for a comprehensive pathway analysis: A case study for retrieval of homocysteine related genes for genetic and epigenetic studies
Sharma, P., Senthilkumar, R., Brahmachari, V., Sundaramoorthy, E., Mahajan, A., Sharma, A., & Sengupta, S. (2006) · Lipids in Health and Disease · doi:10.1186/1476-511x-5-1 · Cited by 171
A Novel Hydrogen Sulfide Prodrug, SG 1002, Promotes Hydrogen Sulfide and Nitric Oxide Bioavailability in Heart Failure Patients
Polhemus, D. J., Li, Z., Pattillo, C. B., Gojon, G., Gojon, G., Giordano, T., & Krum, H. (2015) · Cardiovascular Therapeutics · doi:10.1111/1755-5922.12128 · Cited by 171

Authors: Kuzkaya, N., Weissmann, N., Harrison, D. G., & Dikalov, S.
Affiliations: Emory University (US)
Justus-Liebig-Universität Gießen (DE)
Corresponding author: Dikalov, Sergey
ORCID: Weissmann, Norbert
Harrison, David G.
Dikalov, Sergey
Venue: Journal of Biological Chemistry
Publisher: Elsevier BV
Published: 2003-06
Volume: 278
Issue: 25
Pages: 22546-22554
ISSN: 0021-9258 (ISSN-L)
DOI: 10.1074/jbc.m302227200
OpenAlex: W2058242086
Cited by: 692
References: 50
Citations by year: 2026: 18 · 2025: 15 · 2024: 17 · 2023: 20 · 2022: 23 · 2021: 29 · 2020: 22 · 2019: 24 · 2018: 31 · 2017: 23 · 2016: 24 · 2015: 33 · 2014: 33 · 2013: 25 · 2012: 44
FWCI: 20.60
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
creativecommons.org/licenses/by/4.0/ (vor, 6324 d)
Locations: Journal of Biological Chemistry (publishedVersion, cc-by)
PubMed (publishedVersion)
Funders: Deutsche Forschungsgemeinschaft (SFB-547)
National Institutes of Health (HL058000, PO-1 HL058000)
National Heart, Lung, and Blood Institute (R01HL039006, P0-1 HL058000-06, P01HL058000)
Topics: Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
Keywords: peroxynitrite, tetrahydrobiopterin, vitamin C, thiols, endothelial nitric oxide synthase, eNOS uncoupling, superoxide, nitric oxide, SIN-1, 2,4-diamino-6-hydroxypyrimidine, endothelial cells
MeSH terms: Animals, Aorta, Ascorbic Acid, Biopterins, Cattle, Cells, Cultured, Endothelium, Vascular, Kinetics, Spin Labels, Sulfhydryl Compounds, Nitric Oxide Synthase, Nitric Oxide Donors, Peroxynitrous Acid, Nitric Oxide Synthase Type III

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Interactions of Peroxynitrite, Tetrahydrobiopterin, Ascorbic Acid, and Thiols

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks