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Impaired Micro-RNA Pathways Diminish Osteoclast Differentiation and Function

Sugatani, Toshifumi, Hruska, Keith A.·2009·Journal of Biological Chemistry
DOI 10.1074/jbc.m805777200
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FIGURE 1 Gene silencing and function of DGCR8, Dicer1 or Ago2 in osteoclast precursors. Quantitative real time PCR was performed with primer for mouse DGCR8 ( A ), Dicer1 ( B ), or Ago2 ( C ). The data represent the means ± S.D. of three experiments in triplicate. The loss of DGCR8 ( D ), Dicer1 ( E ), or Ago2 ( F ) protein expression levels by siRNA gene silencing was analyzed by immunoblotting ( IB ) using nuclear or whole cell extracts. Quantitative image analysis of these protein expression levels was normalized to GAPDH ( %scr ). scr , scrambled. miRNA RT-PCR ( G and H ) and quantitative real time RT-PCR ( I ) were performed to measure miRNA-223 and miRNA-155 expression levels. The PCR products were confirmed using a 20% polyacrylamide gel, with U6 small nuclear RNA as a loading control ( G and H ). PCR products were normalized to U6 level for each reaction ( I ). The data represent the means ± S.D. of three experiments in triplicate.
FIGURE 1 Gene silencing and function of DGCR8, Dicer1 or Ago2 in osteoclast precursors. Quantitative real time PCR was performed with primer for mouse DGCR8 ( A ), Dicer1 ( B ), or Ago2 ( C ). The data represent the means ± S.D. of three experiments in triplicate. The loss of DGCR8 ( D ), Dicer1 ( E ), or Ago2 ( F ) protein expression levels by siRNA gene silencing was analyzed by immunoblotting ( IB ) using nuclear or whole cell extracts. Quantitative image analysis of these protein expression levels was normalized to GAPDH ( %scr ). scr , scrambled. miRNA RT-PCR ( G and H ) and quantitative real time RT-PCR ( I ) were performed to measure miRNA-223 and miRNA-155 expression levels. The PCR products were confirmed using a 20% polyacrylamide gel, with U6 small nuclear RNA as a loading control ( G and H ). PCR products were normalized to U6 level for each reaction ( I ). The data represent the means ± S.D. of three experiments in triplicate.CC-BY · From the PDF
FIGURE 2 Expression of transcription factors in osteoclast differentiation. Infected cells were treated with RANKL (50 ng/ml) for 3 days, and nuclear extracts were analyzed by immunoblotting with antibodies against PU.1( A ), MITF ( B ), c-Fos ( C ), and NFATc1 ( D ). Quantitative image analysis of the protein expression levels (values below bands) was normalized to histone H3. IB , immunoblotting.
FIGURE 2 Expression of transcription factors in osteoclast differentiation. Infected cells were treated with RANKL (50 ng/ml) for 3 days, and nuclear extracts were analyzed by immunoblotting with antibodies against PU.1( A ), MITF ( B ), c-Fos ( C ), and NFATc1 ( D ). Quantitative image analysis of the protein expression levels (values below bands) was normalized to histone H3. IB , immunoblotting.CC-BY · From the PDF
FIGURE 3 ChIP analysis. ShownareChIPassays to study the association of NFATc1, c-Fos, PU.1, and MITF with NFATc1 or TRAP promoters during osteoclast differentiation with RANKL (50 ng/ml) stimulation for indicated times.
FIGURE 3 ChIP analysis. ShownareChIPassays to study the association of NFATc1, c-Fos, PU.1, and MITF with NFATc1 or TRAP promoters during osteoclast differentiation with RANKL (50 ng/ml) stimulation for indicated times.CC-BY · From the PDF
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FIGURE 8 Inhibition of miRNA-223 expression causes aberrant osteoclastogenesis. A and B , RAW264.7 cells were transiently transfected with antisense miRNA-223ornegativecontrololigonucleotides,andthecellsweretreatedwithRANKL(100ng/ml).After5days,thecellswerethenfixedandstainedforTRAP, and the number of TRAP-positive multinucleated cells was scored. Similar findings were obtained in four independent sets of experiments. C and D , NFI-A, M-CSFR,andPU.1levelsinRAW264.7cellswithnegativecontrolorantisensemiRNA-223andinfectedosteoclastprecursorswereanalyzedbyimmunoblotting ( IB ) using whole cell extracts. Quantitative image analyses of these protein expression levels were normalized to GAPDH. D , The expression levels of miRNA-223 was measured by miRNA Northern blotting using biotin prelabeled probes specific for miRNA-223. Sno234 was used as a loading control. The sno234 normalized fold increase in the miRNA-223 in osteoclast precursors with pMX-PU.1 retroviral vector is shown.
FIGURE 8 Inhibition of miRNA-223 expression causes aberrant osteoclastogenesis. A and B , RAW264.7 cells were transiently transfected with antisense miRNA-223ornegativecontrololigonucleotides,andthecellsweretreatedwithRANKL(100ng/ml).After5days,thecellswerethenfixedandstainedforTRAP, and the number of TRAP-positive multinucleated cells was scored. Similar findings were obtained in four independent sets of experiments. C and D , NFI-A, M-CSFR,andPU.1levelsinRAW264.7cellswithnegativecontrolorantisensemiRNA-223andinfectedosteoclastprecursorswereanalyzedbyimmunoblotting ( IB ) using whole cell extracts. Quantitative image analyses of these protein expression levels were normalized to GAPDH. D , The expression levels of miRNA-223 was measured by miRNA Northern blotting using biotin prelabeled probes specific for miRNA-223. Sno234 was used as a loading control. The sno234 normalized fold increase in the miRNA-223 in osteoclast precursors with pMX-PU.1 retroviral vector is shown.CC-BY · From the PDF
FIGURE9 Amodelofanovelmechanismforcontrollingosteoclastdifferentiation and function by miRNA-223 in osteoclast precursors. PU.1 induced by M-CSF activates the transcription of miRNA-223, which causes a negative control on M-CSFR expression levels. Also, induced PU.1 stimulates RANK expression, and consequently osteoclast differentiation and function are induced by M-CSF and RANKL stimulation.
FIGURE9 Amodelofanovelmechanismforcontrollingosteoclastdifferentiation and function by miRNA-223 in osteoclast precursors. PU.1 induced by M-CSF activates the transcription of miRNA-223, which causes a negative control on M-CSFR expression levels. Also, induced PU.1 stimulates RANK expression, and consequently osteoclast differentiation and function are induced by M-CSF and RANKL stimulation.CC-BY · From the PDF

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Metadata

Authors
Sugatani, T., & Hruska, K. A.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2009-02
Volume
284
Issue
7
Pages
4667-4678
ISSN
0021-9258
DOI
10.1074/jbc.m805777200
Cited by
196
References
6
Language
en
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