The effect of wild blueberry (Vaccinium angustifolium) consumption on postprandial serum antioxidant status in human subjects
Open accessAbstract
The aim of the present study was to determine whether the consumption of wild blueberries (Vaccinium angustifolium), a concentrated source of non-nutritive antioxidant phytochemicals, would enhance postprandial serum antioxidant status in healthy human subjects. A single-blinded crossover study was performed in a group of eight middle-aged male subjects (38-54 years). Subjects consumed a high-fat meal and a control supplement followed 1 week later by the same high-fat meal supplemented with 100.0 g freeze-dried wild blueberry powder. Upon brachial vein catheterization, fasting and postprandial serum samples were taken sequentially and analysed for lipids and glucose and for serum antioxidant status. Serum antioxidant status was determined using the oxygen radical absorbance capacity (ORAC) assay and the total antioxidant status (TAS) assay. The wild-blueberry treatment was associated with a significant treatment effect as determined by the ORAC assay (water-soluble fraction ORAC(perchloric acid (PCA)), P=0.04). Significant increases in serum antioxidant status above the controls were observed at 1 h (ORAC(PCA) (8.5 % greater), P=0.02; TAS (4.5 % greater), P=0.05), and 4 h (ORAC(total) (15.0 % greater), P=0.009; ORAC(acetone) (16.0 % greater), P=0.007) post-consumption of the high-fat meal. In conclusion, the consumption of wild blueberries, a food source with high in vitro antioxidant properties, is associated with a diet-induced increase in ex vivo serum antioxidant status. It has been suggested that increasing the antioxidant status of serum may result in the reduced risk of many chronic degenerative diseases.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Benefits of berry intervention were seen in healthy subjects and in those with existing metabolic risk factors.
“Benefits were seen in healthy subjects and in those with existing metabolic risk factors.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [38]
Human intervention studies using chokeberries, cranberries, blueberries, and strawberries (fresh, juice, or freeze-dried), or purified anthocyanin extracts have demonstrated significant improvements in LDL oxidation, lipid peroxidation, total plasma antioxidant capacity, dyslipidemia, and glucose metabolism.
“Human intervention studies using chokeberries, cranberries, blueberries, and strawberries (either fresh, or as juice, or freeze-dried), or purified anthocyanin extracts have demonstrated significant improvements in LDL oxidation, lipid peroxidation, total plasma antioxidant capacity, dyslipidemia, and glucose metabolism.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [38]
The most significant outcomes of clinical studies show an increase in plasma or urinary antioxidant capacity, a decrease in LDL oxidation and lipid peroxidation, a decrease in plasma glucose or total cholesterol, and an increase in HDL-cholesterol following berry intervention.
“The most significant outcomes of these clinical studies show an increase in plasma or urinary antioxidant capacity, a decrease in LDL oxidation and lipid peroxidation, a decrease in plasma glucose or total cholesterol, and an increase in HDL-cholesterol following berry intervention.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [38]
Of 20 trials reviewed, nine involved measures of post-prandial status, in which berry consumption was shown to significantly decrease postprandial oxidative stress, especially lipid peroxidation.
“Of 20 trials reviewed, nine involved measures of post-prandial status, in which berry consumption was shown to significantly decrease postprandial oxidative stress, especially lipid peroxidation.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [38]
Clinical studies in healthy humans, subjects with diabetes mellitus, dyslipidemia, metabolic syndrome, hypertension, or in smokers, show a significant decrease in CVD risk factors, especially glucose, lipids and lipid peroxidation, and systolic blood pressure, following berry intervention.
“Clinical studies in healthy humans, subjects with diabetes mellitus, dyslipidemia, metabolic syndrome, hypertension, or in smokers, show a significant decrease in CVD risk factors, especially glucose, lipids and lipid peroxidation, and systolic blood pressure, following berry intervention.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [38]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Friedewald, W. T., Levy, R. I., & Fredrickson, D. S. (1972) · Clinical Chemistry · doi:10.1093/clinchem/18.6.499 · Cited by 32,423 · see citing works
Middleton, E., Kandaswami, C., & Theoharides, T. C. (2000) · Pharmacological Reviews · doi:10.1016/s0031-6997(24)01472-8 · Cited by 4,882 · see citing works
Dietary Intake and Bioavailability of Polyphenols
Scalbert, A., & Williamson, G. (2000) · The Journal of Nutrition · doi:10.1093/jn/130.8.2073s · Cited by 3,658 · see citing works
Vegetables, Fruit, and Cancer Prevention
STEINMETZ, K. A., & POTTER, J. D. (1996) · Journal of the American Dietetic Association · doi:10.1016/s0002-8223(96)00273-8 · Cited by 2,118 · see citing works
Total Antioxidant Capacity of Fruits
Wang, H., Cao, G., & Prior, R. L. (1996) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf950579y · Cited by 1,686 · see citing works
Oxygen-radical absorbance capacity assay for antioxidants
Cao, G., Alessio, H. M., & Cutler, R. G. (1993) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(93)90027-r · Cited by 1,612 · see citing works
Wang, S. Y., & Lin, H.-S. (2000) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf9908345 · Cited by 1,380 · see citing works
The causes and prevention of cancer.
Ames, B. N., Gold, L. S., & Willett, W. C. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.12.5258 · Cited by 1,321 · see citing works
Prior, R. L., Cao, G., Martín, A., Sofic, E., McEwen, J. J., O'Brien, C., Lischner, N., Ehlenfeldt, M. K., Kalt-Torres, W., Krewer, G. W., et al. (1998) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf980145d · Cited by 1,298 · see citing works
Hertog, M. G. L., Hollman, P. C. H., Katan, M. B., & Kromhout, D. (1993) · Nutrition and Cancer · doi:10.1080/01635589309514267 · Cited by 1,216 · see citing works
Dietary Fiber and Weight Regulation
Howarth, N. C., Saltzman, E. J., & Roberts, S. B. (2009) · Nutrition Reviews · doi:10.1111/j.1753-4887.2001.tb07001.x · Cited by 1,053 · see citing works
Antioxidant Capacity, Vitamin C, Phenolics, and Anthocyanins after Fresh Storage of Small Fruits
Kalt, W., Forney, C. F., Martín, A., & Prior, R. L. (1999) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf990266t · Cited by 981 · see citing works
Cancer risk and oxidative DNA damage in man
Loft, S. H., & Poulsen, H. E. (1996) · Journal of Molecular Medicine · doi:10.1007/bf00207507 · Cited by 955 · see citing works
Fruit and vegetable intake and risk of cardiovascular disease: The women’s health study
Liu, S., Manson, J. E., Lee, I.-M., Cole, S. R., Hennekens, C. H., Willett, W. C., & Buring, J. E. (2000) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/72.4.922 · Cited by 917 · see citing works
Comparison of different analytical methods for assessing total antioxidant capacity of human serum
Cao, G., & Prior, R. L. (1998) · Clinical Chemistry · doi:10.1093/clinchem/44.6.1309 · Cited by 904 · see citing works
Functional food science and defence against reactive oxidative species
Diplock, A. T., Charuleux, J.-L., Crozier, G. L., Kok, F. J., Rice-Evans, C. A., Roberfroid, M. B., Stahl, W., & Viña, J. (1998) · British Journal Of Nutrition · doi:10.1079/bjn19980106 · Cited by 811 · see citing works
Francis, F. J., & Markakis, P. (1989) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398909527503 · Cited by 756 · see citing works
(2001) · CRC Press eBooks · doi:10.1201/9780203904046 · Cited by 754 · see citing works
Rice-Evans, C. A. (2001) · Current Medicinal Chemistry · doi:10.2174/0929867013373011 · Cited by 719 · see citing works
In Vitro Study of Possible Role of Dietary Fiber in Lowering Postprandial Serum Glucose
Ou, S., Kwok, K., Li, Y., et al. (2001) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf000574n · Cited by 465 · see citing works
Cross, C. E. (1997) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/65.2.579 · Cited by 429 · see citing works
Anthocyanins as Antioxidants on Human Low-Density Lipoprotein and Lecithin−Liposome Systems
Satué-Gracia, M. T., Heinonen, M., & Frankel, E. N. (1997) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf970234a · Cited by 368 · see citing works
Salonen, J. T., Nyyssönen, K., Salonen, R., Lakka, H. ‐., Kaikkonen, J., Porkkala‐Sarataho, E., Voutilainen, S., Lakka, T. A., Rissanen, T., Leskinen, L., et al. (2000) · Journal of Internal Medicine · doi:10.1046/j.1365-2796.2000.00752.x · Cited by 327 · see citing works
Anthocyanins are absorbed in glycated forms in elderly women: a pharmacokinetic study
Cao, G., Muccitelli, H. U., Sánchez‐Moreno, C., & Prior, R. L. (2001) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/73.5.920 · Cited by 289 · see citing works
An update on the role of free radicals and antioxidant defense in human disease
Vendemiale, G., Grattagliano, I., & Altomare, E. (1999) · International Journal of Clinical & Laboratory Research · doi:10.1007/s005990050063 · Cited by 271 · see citing works
Diet and cancer: one view at the start of the millennium.
Willett, W. C. (2001) · PubMed · Cited by 220 · see citing works
Priemé, H., Loft, S. H., Nyyssönen, K., Salonen, J. T., & Poulsen, H. E. (1997) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/65.2.503 · Cited by 182 · see citing works
High-Energy Diets, Fatty Acids and Endothelial Cell Function: Implications for Atherosclerosis
Hennig, B., Toborek, M. J., & McClain, C. J. (2001) · Journal of the American College of Nutrition · doi:10.1080/07315724.2001.10719021 · Cited by 181 · see citing works
Postprandial triglyceridemia and carotid atherosclerosis in middle-aged subjects
Ryu, J. E., Howard, G., Craven, T. E., Bond, M. G., Hagaman, A. P., & Crouse, J. R. (1992) · Stroke · doi:10.1161/01.str.23.6.823 · Cited by 169 · cited 1× · see citing works
“The rise in triacylglycerol following consumption of a high-fat meal is consistent with results in the literature (Ryu et al. 1992).”
ResultBeneficial health effects of low-digestible carbohydrate consumption
Scheppach, W. M., Luehrs, H., & Menzel, T. (2001) · British Journal Of Nutrition · doi:10.1079/bjn2000259 · Cited by 158 · see citing works
Kaplan, M., & Aviram, M. (1999) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm.1999.118 · Cited by 157 · see citing works
van den Berg, R., van Vliet, T., Broekmans, W. M. R., Cnubben, N. H. P., Vaes, W. H. J., Roza, L., Haenen, G. R. M. M., Bast, A., & van den Berg, H. (2001) · The Journal of Nutrition · doi:10.1093/jn/131.6.1714 · Cited by 144 · cited 2× · see citing works
“Previous research using much higher concentrations (10–45-fold) have shown no effect on serum antioxidant status (Priemé et al. 1997; van den Berg et al. 2001).”
BackgroundChing, S., Beilby, J. P., Rossi, E., et al. (2002) · Journal of Nutrition · doi:10.1093/jn/132.2.303 · Cited by 143 · see citing works
Technical note: a simplified procedure for vitamin E determination in beef muscle.
Liu, Q., Scheller, K. K., & Schaefer, D. M. (1996) · Journal of Animal Science · doi:10.2527/1996.74102406x · Cited by 110 · see citing works
Determinants of antioxidant status in humans
Papas, A. (2019) · Lipids · doi:10.1007/bf02637055 · Cited by 101 · see citing works
A critical assessment of some biomarker approaches linked with dietary intake
Crews, H., Alink, G., Andersen, R., Braesco, V., Holst, B., Maiani, G., Ovesen, L., Scotter, M., Solfrizzo, M., van den Berg, R., et al. (2001) · British Journal Of Nutrition · doi:10.1079/bjn2001337 · Cited by 99 · see citing works
Speek, A. J., Schrijver, J., & Schreurs, W. H. P. (1984) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf00122a043 · Cited by 83 · see citing works
The new pathophysiology of coronary artery disease
Castelli, W. P. (1998) · The American Journal of Cardiology · doi:10.1016/s0002-9149(98)00729-2 · Cited by 76 · see citing works
Fructose transport mechanisms in humans
Shi, X., Schedl, H. P., Summers, R. W., Lambert, G. P., Chang, R., Xia, T., & GISOLFI, C. V. (1997) · Gastroenterology · doi:10.1053/gast.1997.v113.pm9322512 · Cited by 60 · see citing works
Laplaud, P. M., Lelubre, A., & Chapman, M. J. (1997) · Fundamental and Clinical Pharmacology · doi:10.1111/j.1472-8206.1997.tb00166.x · Cited by 53 · see citing works
Oomah, B. D. (2000) · CRC Press eBooks · doi:10.1201/9781482278927 · Cited by 47 · see citing works
Adsorption of Triclopyr on Soil and Some of Its Components
Pusino, A., Liu, W., & Gessa, C. E. (1994) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf00040a036 · Cited by 41 · see citing works
Postprandial increases in serum antioxidant capacity in older women
Cao, G., & Prior, R. L. (2000) · Journal of Applied Physiology · doi:10.1152/jappl.2000.89.3.877 · Cited by 38 · see citing works
Mineral and Vitamin Content of Lowbush Blueberries ( Vaccinium angustifolium Ait.)
Bushway, R. J., GANN, D. F. M., Cook, W. P., et al. (1983) · Journal of Food Science · doi:10.1111/j.1365-2621.1983.tb05109.x · Cited by 36 · see citing works
Overview of Key Nutrients: Micronutrient Aspects
Lachance, P. (2009) · Nutrition Reviews · doi:10.1111/j.1753-4887.1998.tb01714.x · Cited by 31 · see citing works
Chiesa, R., Melissano, G., Castellano, R., et al. (1998) · Annals of Vascular Surgery · doi:10.1007/s100169900107 · Cited by 24 · see citing works
Blood oxidant/antioxidant status of atherosclerotic patients
Durak, İ., Kaçmaz, M., Çimen, M., Büyükkoçak, Ü., & Öztürk, S. (2001) · International Journal of Cardiology · doi:10.1016/s0167-5273(00)00450-2 · Cited by 20 · see citing works
Quantitation of the pathways followed in the conversion of fructose to glucose in liver
Chandramouli, V., Kumaran, K., Ekberg, K., Wahren, J., & Landau, B. R. (1993) · Metabolism · doi:10.1016/0026-0495(93)90192-q · Cited by 17 · see citing works
Digestion and Absorption of Fruit Juice Carbohydrates
Perman, J. A. (1996) · Journal of the American College of Nutrition · doi:10.1080/07315724.1996.10720469 · Cited by 15 · see citing works
Postprandial triglyceride metabolism in diabetes mellitus
Georgopoulos, A. (1999) · Clinical Cardiology · doi:10.1002/clc.4960221406 · Cited by 9 · see citing works
Flagged first, then by citations
Research works printed but not linked: 1
- Speek AJ, Schrijver J & Schreurs WHP (1984) Fluorimetric detection of total vitamin C and total vitamin iso-C in foodstuff and beverages by high performance liquid chromatography with pre-column derivatization. Journal of Agriculture and Food Chemistry 32, 352-355.
Cited by
Sorted by citations
Berries: emerging impact on cardiovascular health
Basu, A., Rhone, M., & Lyons, T. J. (2010) · Nutrition Reviews · doi:10.1111/j.1753-4887.2010.00273.x · Cited by 462 · see citing works
“…± 4.04 years) None 200 mL chokeberry juice (Aronia melanocarpa) Decrease in fasting glucose, HbA1C, and lipids (P < 0.001) in the three-month intervention Kay and Holub (2002)38 Postprandial phases, one week apart Single-blind crossover study Eight middleaged male subjects (mean age, 47 ± 2…”
InfluentialStrawberries, Blueberries, and Cranberries in the Metabolic Syndrome: Clinical Perspectives
Basu, A., & Lyons, T. J. (2012) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf203488k · Cited by 116 · see citing works
- Authors
- Kay, C. D., & Holub, B. J.
- Affiliations
- University of Guelph (CA)
- Corresponding author
- Holub, Bruce J.
- ORCID
- Kay, Colin D.
- Venue
- British Journal Of Nutrition
- Publisher
- Cambridge University Press (CUP)
- Published
- 2002-10
- Volume
- 88
- Issue
- 4
- Pages
- 389-397
- ISSN
- 0007-1145 (ISSN-L)
- 1475-2662
- OpenAlex
- W2100123001
- Cited by
- 199
- References
- 50
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 3 · 2023: 1 · 2022: 4 · 2021: 9 · 2020: 13 · 2019: 8 · 2018: 9 · 2017: 7 · 2016: 9 · 2015: 11 · 2014: 10 · 2013: 16 · 2012: 11
- FWCI
- 3.09
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- British Journal Of Nutrition (publishedVersion)
- PubMed (publishedVersion)
- UEA Digital Repository (University of East Anglia) (submittedVersion)
- Funders
- Ontario Ministry of Agriculture, Food and Rural Affairs
- Ministry of Agriculture, Food and Rural Affairs
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Keywords
- Vaccinium angustifolium, high-fat meal, dietary antioxidants, phytochemicals, ORAC, total antioxidant status
- MeSH terms
- Analysis of Variance, Antioxidants, Ascorbic Acid, Blood Glucose, Diet, Dietary Fats, Dietary Fiber, Humans, Lipids, Male, Middle Aged, Nutritional Status, Vitamin E, Single-Blind Method, Cross-Over Studies, Postprandial Period, Blueberry Plants
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
