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The human gut microbiota in people with amyotrophic lateral sclerosis
Open accessAbstract
Objective To characterize the gut microbiota in people with amyotrophic lateral sclerosis (ALS) relative to controls and to test the hypothesis that butyrate-producing bacteria are less abundant in the gastrointestinal tracts of people with ALS (PALS). Methods: We conducted a case–control study at Massachusetts General Hospital to compare the gut microbiota in people with ALS to that in controls. Metagenomic shotgun sequencing was performed on DNA extracted from stool samples of 66 people with ALS (PALS), 61 healthy controls (HC), and 12 neurodegenerative controls (NDC). Taxonomic metagenomic profiles were analyzed for shifts in the microbial community structure between the comparator groups using per-feature univariate and multivariate association tests. Results: The relative abundance of the dominant butyrate-producing bacteria Eubacterium rectale and Roseburia intestinalis was significantly lower in ALS patients compared to HC. Adjustment for age, sex, and constipation did not materially change the results. The total abundance of 8 dominant species capable of producing butyrate was also significantly lower in ALS compared to HC (p < 0.001). Conclusions: The levels of several butyrate-producing bacteria, which are important for gut integrity and regulation of inflammation, were lower in people with ALS compared to controls. These findings lend support to the inference that the gut microbiota could be a risk factor for ALS. Further investigations are warranted, preferably earlier in the disease with corresponding dietary collection and a longitudinal design.
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“Furthermore, studies have identified reduced butyrate-producing species in ALS patients and SOD1G93A mice.221,222,345,346”
From Microbiota–gut–brain axis and its therapeutic applications in neurodegenerative diseases (2024) · cites this paper as [345]
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Metadata
- Authors
- Nicholson, K., Bjornevik, K., Abu-Ali, G., Chan, J., Cortese, M., Dedi, B., Jeon, M., Xavier, R., Huttenhower, C., Ascherio, A., & Berry, J. D.
- Affiliations
- Massachusetts General Hospital (US)
- Department of Nutrition, Harvard T.H. Chan School of Public Health, Boston, MA, USA
- Broad Institute (US)
- Harvard University (US)
- Brigham and Women's Hospital (US)
- ORCID
- Venue
- Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration
- Publisher
- Informa UK Limited
- Published
- 2020-11-02
- Volume
- 22
- Issue
- 3-4
- Pages
- 186-194
- ISSN
- 2167-8421 (ISSN-L)
- 2167-9223
- OpenAlex
- W3096334056
- Cited by
- 123
- References
- 40
- Citations by year
- 2026: 19 · 2025: 23 · 2024: 28 · 2023: 20 · 2022: 24 · 2021: 8 · 2020: 1
- FWCI
- 4.30
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- ALS Finding a Cure
- Winthrop Family Fund in ALS Sciences
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Health Sciences › Medicine › Clinical Neurology › Amyotrophic Lateral Sclerosis Research
- Keywords
- amyotrophic lateral sclerosis, gut microbiota, butyrate-producing bacteria, Eubacterium rectale, Roseburia intestinalis, microbial community structure, intestinal barrier integrity, inflammation, neurodegenerative diseases, risk factors, constipation
- MeSH terms
- Clostridiales, Eubacteriales, Gastrointestinal Microbiome, Amyotrophic Lateral Sclerosis, Humans, Case-Control Studies
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