The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Establishment of the Dorso-ventral Axis in Xenopus Embryos Is Presaged by Early Asymmetries in β-Catenin That Are Modulated by the Wnt Signaling Pathway
Open accessAbstract
Eggs of Xenopus laevis undergo a postfertilization cortical rotation that specifies the position of the dorso-ventral axis and activates a transplantable dorsal-determining activity in dorsal blastomeres by the 32-cell stage. There have heretofore been no reported dorso-ventral asymmetries in endogenous signaling proteins that may be involved in this dorsal-determining activity during early cleavage stages. We focused on β-catenin as a candidate for an asymmetrically localized dorsal-determining factor since it is both necessary and sufficient for dorsal axis formation. We report that β-catenin displays greater cytoplasmic accumulation on the future dorsal side of the Xenopus embryo by the two-cell stage. This asymmetry persists and increases through early cleavage stages, with β-catenin accumulating in dorsal but not ventral nuclei by the 16- to 32cell stages. We then investigated which potential signaling factors and pathways are capable of modulating the steady-state levels of endogenous β-catenin. Steadystate levels and nuclear accumulation of β-catenin increased in response to ectopic Xenopus Wnt-8 (Xwnt-8) and to the inhibition of glycogen synthase kinase-3, whereas neither Xwnt-5A, BVg1, nor noggin increased β-catenin levels before the mid-blastula stage. As greater levels and nuclear accumulation of β-catenin on the future dorsal side of the embryo correlate with the induction of specific dorsal genes, our data suggest that early asymmetries in β-catenin presage and may specify dorso-ventral differences in gene expression and cell fate. Our data further support the hypothesis that these dorso-ventral differences in β-catenin arise in response to the postfertilization activation of a signaling pathway that involves Xenopus glycogen synthase kinase-3.
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Citations
Cites
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“…of nuclear β-catenin in blastomeres at one pole of the embryo marks the site of gastrulation and specifies the dorsoventral (DV) axis in vertebrates (Larabell et al., 1997; Moon and Kimelman, 1998; Schneider et al., 1996), mediates establishment of the AV axis in lower chordates (Imai et al.,…”
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Metadata
- Authors
- Larabell, C. A., Torres, M., Rowning, B. A., Yost, C., Miller, J. R., Wu, M., Kimelman, D., & Moon, R. T.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1997-03-10
- Volume
- 136
- Issue
- 5
- Pages
- 1123-1136
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2074773822
- Cited by
- 407
- References
- 1
- FWCI
- 13.95
- Type
- journal article
- Language
- en
- Open access
- bronze
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