Converging biofabrication and organoid technologies: the next frontier in hepatic and intestinal tissue engineering?
Open accessAbstract
Adult tissue stem cells can form self-organizing 3D organoids in vitro . Organoids resemble small units of their organ of origin and have great potential for tissue engineering, as well as models of disease. However, current culture technology limits the size, architecture and complexity of organoids. Here, we review the establishment of intestinal and hepatic organoids and discuss how the convergence of organoids and biofabrication technologies can help overcome current limitations, and thereby further advance the translational application of organoids in tissue engineering and regenerative medicine.
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Matrigel originated from mouse cells, which hampers its use in human clinical transplantation due to potential immunogenicity.
“Finally, the fact that Matrigel is originated from mouse cells hampers its use in human clinical transplantation due to potential immunogenicity18.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [18]
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Flagged first, then by citations
Cited by
Sorted by citations
Sun, W., Starly, B., Daly, A. C., Burdick, J. A., Groll, J., Skeldon, G., Shu, W., Sakai, Y., Shinohara, M., Nishikawa, M., et al. (2020) · Biofabrication · doi:10.1088/1758-5090/ab5158 · Cited by 426 · see citing works
Towards organoid culture without Matrigel
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High-throughput automated organoid culture via stem-cell aggregation in microcavity arrays
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Mechano-modulatory synthetic niches for liver organoid derivation
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Intestinal organoids: A new paradigm for engineering intestinal epithelium in vitro
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Liver organoids: established tools for disease modeling and drug development
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- Authors
- Schneeberger, K., Spee, B., Costa, P., Sachs, N., Clevers, H., & Malda, J.
- Affiliations
- Utrecht University (NL)
- University Medical Center Utrecht (NL)
- Venue
- Biofabrication
- Publisher
- IOP Publishing
- Published
- 2017-03-01
- Volume
- 9
- Issue
- 1
- Pages
- 013001
- ISSN
- 1758-5082 (ISSN-L)
- 1758-5090
- PMC
- PMC7116183
- OpenAlex
- W2587808031
- Cited by
- 100
- References
- 99
- Citations by year
- 2026: 3 · 2025: 9 · 2024: 9 · 2023: 17 · 2022: 15 · 2021: 13 · 2020: 16 · 2019: 6 · 2018: 7 · 2017: 5
- FWCI
- 0.75
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biofabrication (publishedVersion)
- PubMed (publishedVersion)
- KNAW Research Portal (The Royal Netherlands Academy of Arts and Sciences) (publishedVersion, other-oa)
- PubMed Central (acceptedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
- Utrecht University Repository (Utrecht University) (submittedVersion, other-oa)
- KNAW research portal (Royal Academy of Art and Sciences (KNAW)) (submittedVersion, other-oa)
- Utrecht University Repository (Utrecht University) (submittedVersion)
- Funders
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek (16004121)
- European Research Council (647426)
- Reumafonds (LLP-12)
- Seventh Framework Programme (309962)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- organoids, hepatic organoids, intestinal organoids, tissue engineering, regenerative medicine, 3D organoid culture, disease models, tissue stem cells
- MeSH terms
- Extracellular Matrix, Humans, Intestines, Liver, Organoids, Regeneration, Stem Cells, Tissue Engineering, Regenerative Medicine, Tissue Scaffolds
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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