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Microglial/Macrophage Polarization Dynamics following Traumatic Brain Injury
Open accessAbstract
Activated microglia and macrophages exert dual beneficial and detrimental roles after central nervous system injury, which are thought to be due to their polarization along a continuum from a classical pro-inflammatory M1-like state to an alternative anti-inflammatory M2-like state. The goal of the present study was to analyze the temporal dynamics of microglia/macrophage polarization within the lesion micro-environment following traumatic brain injury (TBI) using a moderate-level controlled cortical impact (CCI) model in mice. We performed a detailed phenotypic analysis of M1- and M2-like polarized microglia/macrophages, as well as nicotinamide adenine dinucleotide phosphate oxidase (NOX2) expression, through 7 days post-injury using real-time polymerase chain reaction (qPCR), flow cytometry and image analyses. We demonstrated that microglia/macrophages express both M1- and M2-like phenotypic markers early after TBI, but the transient up-regulation of the M2-like phenotype was replaced by a predominant M1- or mixed transitional (Mtran) phenotype that expressed high levels of NOX2 at 7 days post-injury. The shift towards the M1-like and Mtran phenotype was associated with increased cortical and hippocampal neurodegeneration. In a follow up study, we administered a selective NOX2 inhibitor, gp91ds-tat, to CCI mice starting at 24 h post-injury to investigate the relationship between NOX2 and M1-like/Mtran phenotypes. Delayed gp91ds-tat treatment altered M1-/M2-like balance in favor of the anti-inflammatory M2-like phenotype, and significantly reduced oxidative damage in neurons at 7 days post-injury. Therefore, our data suggest that despite M1-like and M2-like polarized microglia/macrophages being activated after TBI, the early M2-like response becomes dysfunctional over time, resulting in development of pathological M1-like and Mtran phenotypes driven by increased NOX2 activity.
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“Of recent interest is how post-injury microglia promote increased oxidative damage leading to neurodegeneration through a NOX2-dependent mechanism [100,117,118].”
From Untangling senescent and damage‐associated microglia in the aging and diseased brain (2023) · cites this paper as [117]
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Metadata
- Authors
- Kumar, A., Alvarez-Croda, D.-M., Stoica, B. A., Faden, A. I., & Loane, D. J.
- Affiliations
- University of Maryland, Baltimore (US)
- Department of Anesthesiology and Shock, Trauma and Anesthesiology Research (STAR) Center, University of Maryland School of Medicine, Baltimore, Maryland.
- Corresponding author
- Loane, David J.
- Venue
- Journal of Neurotrauma
- Publisher
- SAGE Publications
- Published
- 2016-10
- Volume
- 33
- Issue
- 19
- Pages
- 1732-1750
- ISSN
- 0897-7151 (ISSN-L)
- 1557-9042
- PMC
- PMC5065034
- OpenAlex
- W2209119157
- Cited by
- 339
- References
- 52
- Citations by year
- 2026: 14 · 2025: 26 · 2024: 27 · 2023: 30 · 2022: 41 · 2021: 55 · 2020: 39 · 2019: 31 · 2018: 27 · 2017: 24 · 2016: 23
- FWCI
- 17.56
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Neurotrauma (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (AG028747)
- National Institute on Aging (AG028747, P30AG028747)
- National Institute of Neurological Disorders and Stroke (R01NS037313, R01NS082308)
- Topics
- Health Sciences › Medicine › Clinical Neurology › Traumatic Brain Injury and Neurovascular Disturbances
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Health Sciences › Medicine › Clinical Neurology › Traumatic Brain Injury Research
- Keywords
- microglial polarization, macrophage polarization, traumatic brain injury, M1/M2 phenotype, NOX2, controlled cortical impact, neuroinflammation, neurodegeneration, oxidative damage, hippocampus, cortical injury, temporal dynamics
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