TBK1 controls autophagosomal engulfment of polyubiquitinated mitochondria through p62/SQSTM1 phosphorylation
Abstract
Selective autophagy adaptor proteins, including p62/SQSTM1, play pivotal roles in the targeted degradation of ubiquitinated proteins or organelles through the autophagy-lysosome system. However, how autophagy adaptors promote the autophagosomal engulfment of selected substrates is poorly understood. Here, we show that p62 phosphorylation at S403 is required for the efficient autophagosomal engulfment of polyubiquitinated mitochondria during Parkin-dependent mitophagy. p62 is able to interact with Parkin-recruited mitochondria without S403 phosphorylation under mitophagy-inducing conditions, but those mitochondria are not enclosed by autophagosomes. Intriguingly, the S403 phosphorylation occurs only in the early period of mitochondrial depolarization. Optineurin and TANK-binding kinase 1 (TBK1) are transiently recruited to the polyubiquitinated mitochondria, and the activated TBK1 phosphorylates p62 at S403. TBK1 inhibitor, BX795, prevents the p62-mediated autophagosomal engulfment of Parkin-recruited mitochondria. Our results suggest that TBK1-mediated S403 phosphorylation regulates the efficient autophagosomal engulfment of ubiquitinated mitochondria as an immediate response to the mitochondrial depolarization.
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TBK1 amplifies mitophagy signaling by phosphorylating OPTN and SQSTM1, enhancing their binding to ubiquitin and LC3, and promoting TBK1 autophosphorylation in a positive-feedback manner.
“Additionally, TBK1 amplifies the signaling cascade for mitophagy by phosphorylating OPTN and SQSTM1 (which enhances binding to ubiquitin and LC3) and promoting TBK1 autophosphorylation in a positive-feedback manner [58,59].”
From Autophagy and ALS: mechanistic insights and therapeutic implications (2022) · cites this paper as [59]
Through phosphorylation of the autophagy receptors OPTN, SQSTM1, and CALCOCO2/NDP52, TBK1 regulates macroautophagy, xenophagy and mitophagy.
“Through its phosphorylation of the autophagy receptors OPTN, SQSTM1, and CALCOCO2/NDP52 (calcium binding and coiled-coil domain 2) [58,59,194], TBK1 regulates several aspects of autophagy, including macroautophagy, xenophagy and mitophagy [59,90,148,194–196].”
From Autophagy and ALS: mechanistic insights and therapeutic implications (2022) · cites this paper as [59]
Mitochondrial protein ubiquitination promotes mitochondrial recruitment of autophagy regulators and receptors such as TBK1 and optineurin.
“Mitochondrial protein ubiquitination promotes mitochondrial recruitment of autophagy regulators and receptors such as TBK1 and optineurin (Heo et al., 2015, Matsumoto et al., 2015, Richter et al., 2016).”
From A Cell-Based High-Throughput Screening Identified Two Compounds that Enhance PINK1-Parkin Signaling (2020) · cites this paper as Matsumoto et al., 2015
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- Authors
- Matsumoto, G., Shimogori, T., Hattori, N., & Nukina, N.
- Affiliations
- RIKEN Center for Brain Science (JP)
- Juntendo University (JP)
- Corresponding author
- Matsumoto, Gen
- Nukina, Nobuyuki
- Venue
- Human Molecular Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 2015-05-13
- Volume
- 24
- Issue
- 15
- Pages
- 4429-4442
- ISSN
- 0964-6906 (ISSN-L)
- 1460-2083
- OpenAlex
- W2167192779
- Cited by
- 317
- References
- 45
- Citations by year
- 2026: 19 · 2025: 25 · 2024: 21 · 2023: 35 · 2022: 31 · 2021: 31 · 2020: 38 · 2019: 28 · 2018: 39 · 2017: 27 · 2016: 19 · 2015: 4
- FWCI
- 23.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Human Molecular Genetics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science
- Topics
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Health Sciences › Medicine › Neurology › Parkinson's Disease Mechanisms and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
- Keywords
- TBK1, p62, mitophagy, Parkin, optineurin, mitochondrial depolarization, selective autophagy, BX795, autophagy-lysosome system
- MeSH terms
- Sequestosome-1 Protein, Animals, Autophagy, Cell Line, Humans, Mitochondria, Phosphorylation, Protein Serine-Threonine Kinases, Mice, Knockout, Polyubiquitin, Ubiquitin-Protein Ligases, Adaptor Proteins, Signal Transducing, Mice, Mitophagy
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