Cited by 163Bioinformatics and Genomic NetworksGene expression and cancer classificationSingle-cell and spatial transcriptomics

Guidelines for the design, analysis and interpretation of ‘omics’ data: focus on human endometrium

Open access
Altmäe, Signe, Esteban, Francisco J., Stavreus-Evers, Anneli, Simón, Carlos, Giudice, Linda, Lessey, Bruce A., Horcajadas, Jose A., Macklon, Nick S. +5 more·2014·Human Reproduction Update

Abstract

BACKGROUND: 'Omics' high-throughput analyses, including genomics, epigenomics, transcriptomics, proteomics and metabolomics, are widely applied in human endometrial studies. Analysis of endometrial transcriptome patterns in physiological and pathophysiological conditions has been to date the most commonly applied 'omics' technique in human endometrium. As the technologies improve, proteomics holds the next big promise for this field. The 'omics' technologies have undoubtedly advanced our knowledge of human endometrium in relation to fertility and different diseases. Nevertheless, the challenges arising from the vast amount of data generated and the broad variation of 'omics' profiling according to different environments and stimuli make it difficult to assess the validity, reproducibility and interpretation of such 'omics' data. With the expansion of 'omics' analyses in the study of the endometrium, there is a growing need to develop guidelines for the design of studies, and the analysis and interpretation of 'omics' data. METHODS: Systematic review of the literature in PubMed, and references from relevant articles were investigated up to March 2013. RESULTS: The current review aims to provide guidelines for future 'omics' studies on human endometrium, together with a summary of the status and trends, promise and shortcomings in the high-throughput technologies. In addition, the approaches presented here can be adapted to other areas of high-throughput 'omics' studies. CONCLUSION: A highly rigorous approach to future studies, based on the guidelines provided here, is a prerequisite for obtaining data on biological systems which can be shared among researchers worldwide and will ultimately be of clinical benefit.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1093/humupd/dmt048

Cites

200 of 200 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Many centres worldwide have been collecting blood or other body fluids such as peritoneal fluid, endometrial fluid, and menstrual fluid, as well as tissue samples-in particular ectopic and eutopic endometrium-from women with and without endometriosis, for a variety of research purposes.

    “Many centres worldwide have been collecting blood or other body fluids such as peritoneal fluid, endometrial fluid, and menstrual fluid, as well as tissue samples—in particular ectopic and eutopic endometrium—from women with and without endometriosis, for a variety of research purposes [145–147].”

    From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [145]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

1 of the 201 references couldn’t be matched to a known paper.

Research works printed but not linked: 1

  1. Harper MJ. The implantation window. Baillieres Clin Obstet Gynaecol 1992;6:351-371.

Cited by

Sorted by citations

Authors
Altmäe, S., Esteban, F. J., Stavreus-Evers, A., Simón, C., Giudice, L., Lessey, B. A., Horcajadas, J. A., Macklon, N. S., D'Hooghe, T., Campoy, C., Fauser, B. C., Salamonsen, L. A., & Salumets, A.
Affiliations
  • Universidad de Granada (ES)
  • Universidad de Jaén (ES)
  • Uppsala University (SE)
  • Valencian Infertility Institute (ES)
  • University of California, San Francisco (US)
  • Prisma Health (US)
  • Fundacion Agencia Aragonesa para la Investigacion y el Desarrollo (ES)
  • Princess Anne Hospital (GB)
  • University Medical Center Utrecht (NL)
  • Competence Centre on Health Technologies (Estonia) (EE)
Venue
Human Reproduction Update
Published
2013-09-29
Volume
20
Issue
1
Pages
12-28
ISSN
  • 1355-4786 (ISSN-L)
  • 1460-2369
OpenAlex
W2143750395
Cited by
151
References
201
Citations by year
2026: 4 · 2025: 7 · 2024: 15 · 2023: 12 · 2022: 10 · 2021: 9 · 2020: 14 · 2019: 16 · 2018: 14 · 2017: 16 · 2016: 12 · 2015: 16 · 2014: 6
FWCI
1.07
Type
journal article
Language
en
Open access
Licence
other-oa
Locations
  • Human Reproduction Update (publishedVersion, other-oa)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Institutional Repository of the University of Granada (University of Granada) (submittedVersion, other-oa)
  • Adelaide Research & Scholarship (AR&S) (University of Adelaide) (submittedVersion)
  • ePrints Soton (University of Southampton) (submittedVersion)
  • eScholarship (California Digital Library) (submittedVersion)
  • HighWire Press Open Archive (submittedVersion)
Funders
  • European Commission (329812)
  • State Government of Victoria
  • Ettevõtluse Arendamise Sihtasutus (EU324509, EU30020)
  • National Institutes of Health
  • National Health and Medical Research Council (494802, 1002028)
  • National Institute of Child Health and Human Development
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD067721)
  • Eurostars
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
Keywords
human endometrium, omics data, data analysis and interpretation, transcriptomics, proteomics, metabolomics, epigenomics, high-throughput technologies, reproducibility, endometrial disease
MeSH terms
Endometrium, Female, Humans, Embryo Implantation, Guidelines as Topic, Genomics, Systems Biology, Metabolomics, High-Throughput Nucleotide Sequencing

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review