In vitro efficacies of caspofungin or micafungin catheter lock solutions on Candida albicans biofilm growth
Abstract
OBJECTIVES: Caspofungin and micafungin belong to the echinocandins; the mechanism of action of echinocandins is based on the inhibition of (1,3)-beta-D-glucan synthase. The aim of this study was to investigate in vitro the optimal antifungal lock treatment details against a Candida albicans biofilm. METHODS: An in vitro model of a C. albicans (ATCC 3153 or ATCC 66396) biofilm associated with 100% silicone catheters was used. The effectiveness of the antifungal treatment was assayed against biofilms aged 12 h or 5 days, after exposure to caspofungin (2 mg/L) or micafungin (5 mg/L) for 12 h. The durability of the reduction in the biofilm metabolic activity was investigated (1-3 days after echinocandin treatment). The efficacy of caspofungin and micafungin was determined by evaluating a significant decrease (P < 0.0001) in the metabolic activity of biofilm yeasts. RESULTS: The results showed that the tested antifungal agents used as lock solution significantly (P < 0.0001) reduced the metabolic activity of C. albicans, whatever the biofilm maturation stage (12 h or 5 days old biofilms). The reduction in the metabolic activity of biofilm yeasts was maintained, even after 48 h. CONCLUSIONS: These data suggest that caspofungin (2 mg/L) and micafungin (5 mg/L) could represent good candidates for the reduction or control of fungal biofilms associated with silicone medical devices, as part of an antifungal lock. They were able to induce a significant and persistent reduction in the yeast metabolic activity of intermediate and mature biofilms, 12 h and 5 days old, respectively, when used as catheter lock solutions.
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Other approaches under investigation for Candida biofilms include antimicrobial peptides, inhibitors of biofilm matrix components (e.g., extracellular DNA), probiotics, hsp90 inhibition, modulators of quorum sensing, photodynamic therapy, catheter locks, surface coatings, and inhibition of biofilm dispersal.
“These include the use antimicrobial peptides, inhibitors of other components within the biofilm matrix (i.e. extracellular DNA), the use of probiotics, hsp90 inhibition, as well as modulators of quorum sensing [[87], [88], [89], [90], [91], [92], [93]]. An interesting methodology being developed and applied to the Candida biofilm field is that of photodynamic therapy (PDT), where the inhibitory”
From Antifungal therapy of Candida biofilms: Past, present and future (2023) · cites this paper as [98]
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Cites
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- Authors
- Cateau, E., Rodier, M.-H., & Imbert, C.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Cateau, E.
- Venue
- Journal of Antimicrobial Chemotherapy
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-04-01
- Volume
- 62
- Issue
- 1
- Pages
- 153-155
- ISSN
- 0305-7453 (ISSN-L)
- 1460-2091
- OpenAlex
- W2097132369
- Cited by
- 98
- References
- 10
- Citations by year
- 2026: 1 · 2024: 4 · 2023: 1 · 2022: 4 · 2021: 5 · 2020: 1 · 2019: 2 · 2018: 8 · 2017: 1 · 2016: 6 · 2015: 5 · 2014: 8 · 2013: 11 · 2012: 6
- FWCI
- 7.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Antimicrobial Chemotherapy (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- Astellas Pharma
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Health Sciences › Medicine › Epidemiology › Infective Endocarditis Diagnosis and Management
- Keywords
- caspofungin, micafungin, echinocandins, Candida albicans, fungal biofilms, catheter lock solutions, silicone catheters, biofilm metabolic activity, biofilm maturation
- MeSH terms
- Caspofungin, Micafungin, Antifungal Agents, Candida albicans, Candidiasis, Catheterization, Equipment and Supplies, Humans, Lipoproteins, Silicones, Time Factors, Biofilms, Echinocandins, Lipopeptides
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